Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2899 and Pf6N2E2_2900 are separated by 247 nucleotides Pf6N2E2_2900 and Pf6N2E2_2901 are separated by 156 nucleotides
Pf6N2E2_2899 - NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12), at 3,388,931 to 3,389,932
_2899
Pf6N2E2_2900 - FIG006045: Sigma factor, ECF subfamily, at 3,390,180 to 3,390,701
_2900
Pf6N2E2_2901 - Iron siderophore sensor protein, at 3,390,858 to 3,391,763
_2901
Position (kb)
3390
3391 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3389.266 kb on + strand, within Pf6N2E2_2899 at 3389.267 kb on - strand, within Pf6N2E2_2899 at 3389.267 kb on - strand, within Pf6N2E2_2899 at 3389.333 kb on + strand, within Pf6N2E2_2899 at 3389.477 kb on + strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.478 kb on - strand, within Pf6N2E2_2899 at 3389.507 kb on + strand, within Pf6N2E2_2899 at 3389.508 kb on - strand, within Pf6N2E2_2899 at 3389.864 kb on + strand at 3389.864 kb on + strand at 3389.864 kb on + strand at 3389.864 kb on + strand at 3389.865 kb on - strand at 3389.902 kb on + strand at 3389.930 kb on + strand at 3389.930 kb on + strand at 3389.931 kb on - strand at 3389.931 kb on - strand at 3389.963 kb on + strand at 3389.963 kb on + strand at 3389.964 kb on - strand at 3389.964 kb on - strand at 3390.294 kb on + strand, within Pf6N2E2_2900 at 3390.295 kb on - strand, within Pf6N2E2_2900 at 3390.431 kb on - strand, within Pf6N2E2_2900 at 3390.588 kb on + strand, within Pf6N2E2_2900 at 3390.589 kb on - strand, within Pf6N2E2_2900 at 3390.589 kb on - strand, within Pf6N2E2_2900 at 3390.683 kb on + strand at 3390.724 kb on + strand at 3390.724 kb on + strand at 3390.725 kb on - strand at 3390.725 kb on - strand at 3390.725 kb on - strand at 3390.725 kb on - strand at 3390.734 kb on - strand at 3390.755 kb on + strand at 3390.755 kb on + strand at 3390.756 kb on - strand at 3390.756 kb on - strand at 3390.764 kb on + strand at 3390.765 kb on - strand at 3390.765 kb on - strand at 3390.783 kb on + strand at 3390.784 kb on - strand at 3390.787 kb on - strand at 3391.269 kb on + strand, within Pf6N2E2_2901 at 3391.270 kb on - strand, within Pf6N2E2_2901 at 3391.270 kb on - strand, within Pf6N2E2_2901 at 3391.585 kb on + strand, within Pf6N2E2_2901 at 3391.585 kb on + strand, within Pf6N2E2_2901 at 3391.605 kb on + strand, within Pf6N2E2_2901 at 3391.606 kb on - strand, within Pf6N2E2_2901
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 3,389,266 + Pf6N2E2_2899 0.33 +0.4 3,389,267 - Pf6N2E2_2899 0.34 +0.4 3,389,267 - Pf6N2E2_2899 0.34 +0.8 3,389,333 + Pf6N2E2_2899 0.40 -0.6 3,389,477 + Pf6N2E2_2899 0.54 -0.0 3,389,478 - Pf6N2E2_2899 0.55 +0.4 3,389,478 - Pf6N2E2_2899 0.55 +0.4 3,389,478 - Pf6N2E2_2899 0.55 +0.4 3,389,478 - Pf6N2E2_2899 0.55 -0.1 3,389,507 + Pf6N2E2_2899 0.57 +0.4 3,389,508 - Pf6N2E2_2899 0.58 -1.6 3,389,864 + -0.9 3,389,864 + -0.7 3,389,864 + -0.4 3,389,864 + +0.2 3,389,865 - +0.4 3,389,902 + -1.9 3,389,930 + -1.6 3,389,930 + +0.4 3,389,931 - -0.6 3,389,931 - +0.4 3,389,963 + +0.4 3,389,963 + -0.6 3,389,964 - -1.3 3,389,964 - -1.2 3,390,294 + Pf6N2E2_2900 0.22 -1.2 3,390,295 - Pf6N2E2_2900 0.22 -1.2 3,390,431 - Pf6N2E2_2900 0.48 +0.4 3,390,588 + Pf6N2E2_2900 0.78 +1.0 3,390,589 - Pf6N2E2_2900 0.78 +2.0 3,390,589 - Pf6N2E2_2900 0.78 -0.5 3,390,683 + -1.2 3,390,724 + +0.4 3,390,724 + +0.1 3,390,725 - +0.4 3,390,725 - +0.4 3,390,725 - -0.6 3,390,725 - +0.4 3,390,734 - +0.4 3,390,755 + -1.2 3,390,755 + +0.4 3,390,756 - +0.4 3,390,756 - +0.4 3,390,764 + -0.2 3,390,765 - +0.4 3,390,765 - -0.4 3,390,783 + +0.4 3,390,784 - +1.0 3,390,787 - -0.6 3,391,269 + Pf6N2E2_2901 0.45 -0.6 3,391,270 - Pf6N2E2_2901 0.45 -0.9 3,391,270 - Pf6N2E2_2901 0.45 -1.2 3,391,585 + Pf6N2E2_2901 0.80 -0.7 3,391,585 + Pf6N2E2_2901 0.80 +0.4 3,391,605 + Pf6N2E2_2901 0.82 -1.3 3,391,606 - Pf6N2E2_2901 0.83 +0.4
Or see this region's nucleotide sequence