Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2324

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2321 and Pf6N2E2_2322 overlap by 8 nucleotidesPf6N2E2_2322 and Pf6N2E2_2323 overlap by 42 nucleotidesPf6N2E2_2323 and Pf6N2E2_2324 are separated by 76 nucleotidesPf6N2E2_2324 and Pf6N2E2_2325 are separated by 123 nucleotidesPf6N2E2_2325 and Pf6N2E2_2326 are separated by 48 nucleotides Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615 _2321 Pf6N2E2_2322 - hypothetical protein, at 2,745,608 to 2,745,751 _2322 Pf6N2E2_2323 - Fumarate and nitrate reduction regulatory protein, at 2,745,710 to 2,746,444 _2323 Pf6N2E2_2324 - Adenine phosphoribosyltransferase (EC 2.4.2.7), at 2,746,521 to 2,747,069 _2324 Pf6N2E2_2325 - Recombination protein RecR, at 2,747,193 to 2,747,813 _2325 Pf6N2E2_2326 - Putative oxidoreductase YncB, at 2,747,862 to 2,748,896 _2326 Position (kb) 2746 2747 2748Strain fitness (log2 ratio) -3 -2 -1 0 1at 2745.571 kb on + strandat 2745.572 kb on - strandat 2745.572 kb on - strandat 2745.610 kb on - strandat 2745.705 kb on - strand, within Pf6N2E2_2322at 2746.095 kb on + strand, within Pf6N2E2_2323at 2746.301 kb on + strand, within Pf6N2E2_2323at 2746.301 kb on + strand, within Pf6N2E2_2323at 2746.302 kb on - strand, within Pf6N2E2_2323at 2746.302 kb on - strand, within Pf6N2E2_2323at 2746.482 kb on - strandat 2746.484 kb on - strandat 2746.484 kb on - strandat 2746.605 kb on - strand, within Pf6N2E2_2324at 2746.666 kb on - strand, within Pf6N2E2_2324at 2746.738 kb on - strand, within Pf6N2E2_2324at 2746.854 kb on - strand, within Pf6N2E2_2324at 2747.067 kb on + strandat 2747.067 kb on + strandat 2747.067 kb on + strandat 2747.121 kb on - strandat 2747.180 kb on + strandat 2747.180 kb on + strandat 2747.180 kb on + strandat 2747.181 kb on - strandat 2747.181 kb on - strandat 2747.181 kb on - strandat 2747.181 kb on - strandat 2747.188 kb on - strandat 2747.194 kb on + strandat 2747.195 kb on - strandat 2747.333 kb on - strand, within Pf6N2E2_2325at 2747.333 kb on - strand, within Pf6N2E2_2325at 2747.333 kb on - strand, within Pf6N2E2_2325at 2747.333 kb on - strand, within Pf6N2E2_2325at 2747.333 kb on - strand, within Pf6N2E2_2325at 2747.488 kb on + strand, within Pf6N2E2_2325at 2747.521 kb on + strand, within Pf6N2E2_2325at 2747.521 kb on + strand, within Pf6N2E2_2325at 2747.521 kb on + strand, within Pf6N2E2_2325at 2747.521 kb on + strand, within Pf6N2E2_2325at 2747.522 kb on - strand, within Pf6N2E2_2325at 2747.522 kb on - strand, within Pf6N2E2_2325at 2747.635 kb on - strand, within Pf6N2E2_2325at 2747.737 kb on - strand, within Pf6N2E2_2325at 2747.737 kb on - strand, within Pf6N2E2_2325at 2747.737 kb on - strand, within Pf6N2E2_2325at 2747.813 kb on + strandat 2747.813 kb on + strandat 2747.813 kb on + strandat 2747.813 kb on + strandat 2747.814 kb on - strandat 2747.814 kb on - strandat 2747.832 kb on - strandat 2747.832 kb on - strandat 2747.834 kb on - strandat 2747.860 kb on - strandat 2748.035 kb on - strand, within Pf6N2E2_2326at 2748.051 kb on - strand, within Pf6N2E2_2326

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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2,745,571 + +0.4
2,745,572 - +0.4
2,745,572 - +0.4
2,745,610 - +0.7
2,745,705 - Pf6N2E2_2322 0.67 -0.6
2,746,095 + Pf6N2E2_2323 0.52 -0.6
2,746,301 + Pf6N2E2_2323 0.80 -1.6
2,746,301 + Pf6N2E2_2323 0.80 +0.4
2,746,302 - Pf6N2E2_2323 0.81 +0.4
2,746,302 - Pf6N2E2_2323 0.81 +0.4
2,746,482 - -0.7
2,746,484 - +0.4
2,746,484 - +0.4
2,746,605 - Pf6N2E2_2324 0.15 -1.2
2,746,666 - Pf6N2E2_2324 0.26 -1.6
2,746,738 - Pf6N2E2_2324 0.40 +0.4
2,746,854 - Pf6N2E2_2324 0.61 -3.1
2,747,067 + -0.2
2,747,067 + +0.4
2,747,067 + -0.7
2,747,121 - +1.7
2,747,180 + +0.4
2,747,180 + +0.4
2,747,180 + -0.2
2,747,181 - -3.1
2,747,181 - -0.9
2,747,181 - -1.2
2,747,181 - -0.6
2,747,188 - -0.6
2,747,194 + +0.4
2,747,195 - -1.2
2,747,333 - Pf6N2E2_2325 0.23 -1.5
2,747,333 - Pf6N2E2_2325 0.23 +0.4
2,747,333 - Pf6N2E2_2325 0.23 -0.6
2,747,333 - Pf6N2E2_2325 0.23 -1.3
2,747,333 - Pf6N2E2_2325 0.23 +0.4
2,747,488 + Pf6N2E2_2325 0.48 -0.6
2,747,521 + Pf6N2E2_2325 0.53 +0.4
2,747,521 + Pf6N2E2_2325 0.53 +0.4
2,747,521 + Pf6N2E2_2325 0.53 +0.4
2,747,521 + Pf6N2E2_2325 0.53 +0.4
2,747,522 - Pf6N2E2_2325 0.53 +0.4
2,747,522 - Pf6N2E2_2325 0.53 -1.4
2,747,635 - Pf6N2E2_2325 0.71 -0.6
2,747,737 - Pf6N2E2_2325 0.88 +0.4
2,747,737 - Pf6N2E2_2325 0.88 -0.6
2,747,737 - Pf6N2E2_2325 0.88 +1.4
2,747,813 + +0.4
2,747,813 + +0.4
2,747,813 + +0.4
2,747,813 + -0.6
2,747,814 - +0.4
2,747,814 - +0.4
2,747,832 - +0.4
2,747,832 - +0.4
2,747,834 - -0.9
2,747,860 - -1.4
2,748,035 - Pf6N2E2_2326 0.17 +1.4
2,748,051 - Pf6N2E2_2326 0.18 -0.9

Or see this region's nucleotide sequence