Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2175 and Pf6N2E2_2176 are separated by 2 nucleotides Pf6N2E2_2176 and Pf6N2E2_2177 overlap by 4 nucleotides Pf6N2E2_2177 and Pf6N2E2_2178 overlap by 17 nucleotides Pf6N2E2_2178 and Pf6N2E2_2179 are separated by 76 nucleotides
Pf6N2E2_2175 - Transcriptional regulator, RpiR family, at 2,580,015 to 2,580,869
_2175
Pf6N2E2_2176 - Amidases related to nicotinamidase, at 2,580,872 to 2,581,516
_2176
Pf6N2E2_2177 - Glutamine synthetase (EC 6.3.1.2), putative, at 2,581,513 to 2,582,868
_2177
Pf6N2E2_2178 - FIG00960144: hypothetical protein, at 2,582,852 to 2,583,604
_2178
Pf6N2E2_2179 - Putrescine importer, at 2,583,681 to 2,585,030
_2179
Position (kb)
2581
2582
2583 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2580.660 kb on + strand, within Pf6N2E2_2175 at 2580.867 kb on + strand at 2580.867 kb on + strand at 2581.008 kb on - strand, within Pf6N2E2_2176 at 2581.277 kb on + strand, within Pf6N2E2_2176 at 2581.412 kb on + strand, within Pf6N2E2_2176 at 2581.413 kb on - strand, within Pf6N2E2_2176 at 2581.457 kb on + strand at 2581.633 kb on + strand at 2581.633 kb on + strand at 2581.634 kb on - strand at 2581.634 kb on - strand at 2581.634 kb on - strand at 2581.721 kb on - strand, within Pf6N2E2_2177 at 2581.807 kb on - strand, within Pf6N2E2_2177 at 2582.092 kb on + strand, within Pf6N2E2_2177 at 2582.151 kb on - strand, within Pf6N2E2_2177 at 2582.353 kb on + strand, within Pf6N2E2_2177 at 2582.398 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.434 kb on + strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.435 kb on - strand, within Pf6N2E2_2177 at 2582.782 kb on + strand at 2582.782 kb on + strand at 2582.782 kb on + strand at 2582.783 kb on - strand at 2582.851 kb on + strand at 2582.851 kb on + strand at 2582.851 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.863 kb on + strand at 2582.864 kb on - strand at 2582.888 kb on + strand at 2582.888 kb on + strand at 2582.889 kb on - strand at 2582.889 kb on - strand at 2583.101 kb on + strand, within Pf6N2E2_2178 at 2583.101 kb on + strand, within Pf6N2E2_2178 at 2583.151 kb on + strand, within Pf6N2E2_2178 at 2583.151 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.173 kb on + strand, within Pf6N2E2_2178 at 2583.174 kb on - strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.338 kb on + strand, within Pf6N2E2_2178 at 2583.392 kb on + strand, within Pf6N2E2_2178 at 2583.392 kb on + strand, within Pf6N2E2_2178 at 2583.455 kb on + strand, within Pf6N2E2_2178 at 2583.455 kb on + strand, within Pf6N2E2_2178 at 2583.584 kb on + strand at 2583.585 kb on - strand at 2583.602 kb on + strand at 2583.602 kb on + strand at 2583.602 kb on + strand at 2583.748 kb on + strand at 2583.748 kb on + strand at 2583.749 kb on - strand at 2583.792 kb on + strand at 2583.798 kb on + strand at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179 at 2583.838 kb on - strand, within Pf6N2E2_2179
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 2,580,660 + Pf6N2E2_2175 0.75 +0.3 2,580,867 + -2.0 2,580,867 + +0.3 2,581,008 - Pf6N2E2_2176 0.21 -0.1 2,581,277 + Pf6N2E2_2176 0.63 +0.3 2,581,412 + Pf6N2E2_2176 0.84 -0.7 2,581,413 - Pf6N2E2_2176 0.84 +0.3 2,581,457 + +0.3 2,581,633 + -0.7 2,581,633 + -0.1 2,581,634 - -0.2 2,581,634 - +0.1 2,581,634 - -0.2 2,581,721 - Pf6N2E2_2177 0.15 -0.5 2,581,807 - Pf6N2E2_2177 0.22 -0.4 2,582,092 + Pf6N2E2_2177 0.43 -2.2 2,582,151 - Pf6N2E2_2177 0.47 +0.3 2,582,353 + Pf6N2E2_2177 0.62 +0.3 2,582,398 + Pf6N2E2_2177 0.65 -1.2 2,582,434 + Pf6N2E2_2177 0.68 -1.2 2,582,434 + Pf6N2E2_2177 0.68 +0.3 2,582,434 + Pf6N2E2_2177 0.68 -0.4 2,582,435 - Pf6N2E2_2177 0.68 -1.5 2,582,435 - Pf6N2E2_2177 0.68 +0.3 2,582,435 - Pf6N2E2_2177 0.68 +0.3 2,582,435 - Pf6N2E2_2177 0.68 -1.7 2,582,782 + -2.2 2,582,782 + +0.3 2,582,782 + +0.9 2,582,783 - -0.7 2,582,851 + +0.3 2,582,851 + -0.6 2,582,851 + -0.7 2,582,863 + +0.3 2,582,863 + +0.3 2,582,863 + -1.7 2,582,863 + +0.3 2,582,864 - -0.1 2,582,888 + +0.3 2,582,888 + -0.8 2,582,889 - +0.3 2,582,889 - -0.2 2,583,101 + Pf6N2E2_2178 0.33 -0.7 2,583,101 + Pf6N2E2_2178 0.33 -1.7 2,583,151 + Pf6N2E2_2178 0.40 -0.7 2,583,151 + Pf6N2E2_2178 0.40 -0.7 2,583,173 + Pf6N2E2_2178 0.43 -0.5 2,583,173 + Pf6N2E2_2178 0.43 +0.3 2,583,173 + Pf6N2E2_2178 0.43 -0.7 2,583,173 + Pf6N2E2_2178 0.43 -1.2 2,583,174 - Pf6N2E2_2178 0.43 +0.3 2,583,338 + Pf6N2E2_2178 0.65 +0.3 2,583,338 + Pf6N2E2_2178 0.65 +0.3 2,583,338 + Pf6N2E2_2178 0.65 -0.7 2,583,338 + Pf6N2E2_2178 0.65 +0.3 2,583,392 + Pf6N2E2_2178 0.72 +0.3 2,583,392 + Pf6N2E2_2178 0.72 +0.3 2,583,455 + Pf6N2E2_2178 0.80 +0.3 2,583,455 + Pf6N2E2_2178 0.80 -1.2 2,583,584 + +0.3 2,583,585 - +1.9 2,583,602 + +0.3 2,583,602 + -0.7 2,583,602 + -0.7 2,583,748 + +0.3 2,583,748 + -0.2 2,583,749 - -0.7 2,583,792 + +0.3 2,583,798 + +0.3 2,583,838 - Pf6N2E2_2179 0.12 +0.3 2,583,838 - Pf6N2E2_2179 0.12 +0.3 2,583,838 - Pf6N2E2_2179 0.12 +0.3 2,583,838 - Pf6N2E2_2179 0.12 -2.2
Or see this region's nucleotide sequence