Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2137 and Pf6N2E2_2138 are separated by 323 nucleotides Pf6N2E2_2138 and Pf6N2E2_2139 are separated by 69 nucleotides Pf6N2E2_2139 and Pf6N2E2_2140 overlap by 4 nucleotides Pf6N2E2_2140 and Pf6N2E2_2141 overlap by 20 nucleotides
Pf6N2E2_2137 - DNA polymerase II (EC 2.7.7.7), at 2,542,575 to 2,544,938
_2137
Pf6N2E2_2138 - Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205), at 2,545,262 to 2,545,702
_2138
Pf6N2E2_2139 - Paraquat-inducible protein A, at 2,545,772 to 2,546,368
_2139
Pf6N2E2_2140 - Paraquat-inducible protein A, at 2,546,365 to 2,547,024
_2140
Pf6N2E2_2141 - Paraquat-inducible protein B, at 2,547,005 to 2,548,663
_2141
Position (kb)
2545
2546
2547 Strain fitness (log2 ratio)
-2
-1
0
1 at 2544.930 kb on + strand at 2544.930 kb on + strand at 2544.974 kb on + strand at 2544.975 kb on - strand at 2544.975 kb on - strand at 2545.218 kb on - strand at 2545.218 kb on - strand at 2545.227 kb on + strand at 2545.228 kb on - strand at 2545.228 kb on - strand at 2545.290 kb on + strand at 2545.291 kb on - strand at 2545.341 kb on + strand, within Pf6N2E2_2138 at 2545.341 kb on + strand, within Pf6N2E2_2138 at 2545.341 kb on + strand, within Pf6N2E2_2138 at 2545.342 kb on - strand, within Pf6N2E2_2138 at 2545.342 kb on - strand, within Pf6N2E2_2138 at 2545.436 kb on + strand, within Pf6N2E2_2138 at 2545.437 kb on - strand, within Pf6N2E2_2138 at 2545.685 kb on + strand at 2545.685 kb on + strand at 2545.685 kb on + strand at 2545.686 kb on - strand at 2545.686 kb on - strand at 2545.686 kb on - strand at 2545.686 kb on - strand at 2545.739 kb on + strand at 2545.739 kb on + strand at 2545.739 kb on + strand at 2545.739 kb on + strand at 2545.739 kb on + strand at 2545.739 kb on + strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.740 kb on - strand at 2545.742 kb on - strand at 2545.818 kb on + strand at 2545.819 kb on - strand at 2545.820 kb on + strand at 2545.821 kb on - strand at 2545.889 kb on + strand, within Pf6N2E2_2139 at 2545.965 kb on - strand, within Pf6N2E2_2139 at 2546.138 kb on + strand, within Pf6N2E2_2139 at 2546.139 kb on - strand, within Pf6N2E2_2139 at 2546.533 kb on + strand, within Pf6N2E2_2140 at 2546.533 kb on + strand, within Pf6N2E2_2140 at 2546.533 kb on + strand, within Pf6N2E2_2140 at 2546.534 kb on - strand, within Pf6N2E2_2140 at 2546.534 kb on - strand, within Pf6N2E2_2140 at 2546.557 kb on + strand, within Pf6N2E2_2140 at 2546.557 kb on + strand, within Pf6N2E2_2140 at 2546.558 kb on - strand, within Pf6N2E2_2140 at 2546.680 kb on - strand, within Pf6N2E2_2140 at 2547.351 kb on + strand, within Pf6N2E2_2141
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 2,544,930 + +0.3 2,544,930 + -0.3 2,544,974 + +0.3 2,544,975 - -0.3 2,544,975 - -1.3 2,545,218 - +0.3 2,545,218 - -0.7 2,545,227 + -0.4 2,545,228 - +0.3 2,545,228 - +0.3 2,545,290 + -0.7 2,545,291 - +0.3 2,545,341 + Pf6N2E2_2138 0.18 -1.0 2,545,341 + Pf6N2E2_2138 0.18 -0.3 2,545,341 + Pf6N2E2_2138 0.18 -0.6 2,545,342 - Pf6N2E2_2138 0.18 -0.3 2,545,342 - Pf6N2E2_2138 0.18 -0.5 2,545,436 + Pf6N2E2_2138 0.39 +0.3 2,545,437 - Pf6N2E2_2138 0.40 -1.7 2,545,685 + +0.3 2,545,685 + -0.8 2,545,685 + -0.7 2,545,686 - +1.1 2,545,686 - -0.7 2,545,686 - +0.3 2,545,686 - +0.3 2,545,739 + -1.3 2,545,739 + +0.3 2,545,739 + -0.7 2,545,739 + -1.3 2,545,739 + +1.3 2,545,739 + -1.7 2,545,740 - +0.3 2,545,740 - -2.0 2,545,740 - +0.3 2,545,740 - -0.4 2,545,740 - -1.7 2,545,740 - +0.3 2,545,740 - +0.3 2,545,740 - -0.7 2,545,740 - +0.3 2,545,740 - -0.7 2,545,740 - +0.3 2,545,740 - -0.4 2,545,742 - +0.0 2,545,818 + -0.7 2,545,819 - +0.3 2,545,820 + +0.3 2,545,821 - -0.7 2,545,889 + Pf6N2E2_2139 0.20 +0.3 2,545,965 - Pf6N2E2_2139 0.32 -2.6 2,546,138 + Pf6N2E2_2139 0.61 -0.7 2,546,139 - Pf6N2E2_2139 0.61 +0.3 2,546,533 + Pf6N2E2_2140 0.25 -0.7 2,546,533 + Pf6N2E2_2140 0.25 +0.3 2,546,533 + Pf6N2E2_2140 0.25 -1.1 2,546,534 - Pf6N2E2_2140 0.26 -0.7 2,546,534 - Pf6N2E2_2140 0.26 -0.7 2,546,557 + Pf6N2E2_2140 0.29 +0.3 2,546,557 + Pf6N2E2_2140 0.29 +0.3 2,546,558 - Pf6N2E2_2140 0.29 +0.3 2,546,680 - Pf6N2E2_2140 0.48 -0.4 2,547,351 + Pf6N2E2_2141 0.21 -0.7
Or see this region's nucleotide sequence