Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1864

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1863 and Pf6N2E2_1864 are separated by 416 nucleotidesPf6N2E2_1864 and Pf6N2E2_1865 are separated by 17 nucleotidesPf6N2E2_1865 and Pf6N2E2_1866 are separated by 14 nucleotides Pf6N2E2_1863 - putative dioxygenase hydroxylase component, at 2,206,425 to 2,207,684 _1863 Pf6N2E2_1864 - Transcriptional regulator, TetR family, at 2,208,101 to 2,208,751 _1864 Pf6N2E2_1865 - Beta-carotene hydroxylase, at 2,208,769 to 2,209,659 _1865 Pf6N2E2_1866 - Rubredoxin, at 2,209,674 to 2,209,835 _1866 Position (kb) 2208 2209Strain fitness (log2 ratio) -2 -1 0 1 2at 2207.251 kb on + strand, within Pf6N2E2_1863at 2207.369 kb on - strand, within Pf6N2E2_1863at 2207.379 kb on - strand, within Pf6N2E2_1863at 2207.379 kb on - strand, within Pf6N2E2_1863at 2207.811 kb on - strandat 2207.850 kb on - strandat 2207.850 kb on - strandat 2207.988 kb on + strandat 2207.989 kb on - strandat 2207.989 kb on - strandat 2207.989 kb on - strandat 2208.043 kb on + strandat 2208.116 kb on + strandat 2208.176 kb on + strand, within Pf6N2E2_1864at 2208.190 kb on + strand, within Pf6N2E2_1864at 2208.191 kb on - strand, within Pf6N2E2_1864at 2208.191 kb on - strand, within Pf6N2E2_1864at 2208.269 kb on - strand, within Pf6N2E2_1864at 2208.315 kb on + strand, within Pf6N2E2_1864at 2208.564 kb on + strand, within Pf6N2E2_1864at 2208.564 kb on + strand, within Pf6N2E2_1864at 2208.565 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.569 kb on - strand, within Pf6N2E2_1864at 2208.579 kb on + strand, within Pf6N2E2_1864at 2208.746 kb on - strandat 2208.746 kb on - strandat 2208.754 kb on + strandat 2208.755 kb on - strandat 2208.775 kb on - strandat 2208.929 kb on - strand, within Pf6N2E2_1865at 2208.960 kb on + strand, within Pf6N2E2_1865at 2208.960 kb on + strand, within Pf6N2E2_1865at 2208.973 kb on + strand, within Pf6N2E2_1865at 2208.973 kb on + strand, within Pf6N2E2_1865at 2209.301 kb on + strand, within Pf6N2E2_1865at 2209.318 kb on + strand, within Pf6N2E2_1865at 2209.319 kb on - strand, within Pf6N2E2_1865at 2209.319 kb on - strand, within Pf6N2E2_1865at 2209.455 kb on + strand, within Pf6N2E2_1865at 2209.513 kb on + strand, within Pf6N2E2_1865at 2209.513 kb on + strand, within Pf6N2E2_1865at 2209.514 kb on - strand, within Pf6N2E2_1865at 2209.514 kb on - strand, within Pf6N2E2_1865at 2209.592 kb on + strandat 2209.592 kb on + strandat 2209.711 kb on - strand, within Pf6N2E2_1866

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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2,207,251 + Pf6N2E2_1863 0.66 -0.7
2,207,369 - Pf6N2E2_1863 0.75 +0.3
2,207,379 - Pf6N2E2_1863 0.76 +0.3
2,207,379 - Pf6N2E2_1863 0.76 +0.3
2,207,811 - -0.3
2,207,850 - -0.8
2,207,850 - -0.6
2,207,988 + +0.3
2,207,989 - +0.3
2,207,989 - +1.9
2,207,989 - -1.2
2,208,043 + -1.5
2,208,116 + -1.3
2,208,176 + Pf6N2E2_1864 0.12 -1.3
2,208,190 + Pf6N2E2_1864 0.14 +0.3
2,208,191 - Pf6N2E2_1864 0.14 -0.7
2,208,191 - Pf6N2E2_1864 0.14 -0.6
2,208,269 - Pf6N2E2_1864 0.26 -1.3
2,208,315 + Pf6N2E2_1864 0.33 -0.7
2,208,564 + Pf6N2E2_1864 0.71 +0.3
2,208,564 + Pf6N2E2_1864 0.71 -0.7
2,208,565 - Pf6N2E2_1864 0.71 +0.3
2,208,569 - Pf6N2E2_1864 0.72 -1.3
2,208,569 - Pf6N2E2_1864 0.72 +0.3
2,208,569 - Pf6N2E2_1864 0.72 -1.3
2,208,569 - Pf6N2E2_1864 0.72 +0.3
2,208,579 + Pf6N2E2_1864 0.73 -2.7
2,208,746 - +0.3
2,208,746 - +0.3
2,208,754 + -0.3
2,208,755 - +0.3
2,208,775 - +0.3
2,208,929 - Pf6N2E2_1865 0.18 +0.3
2,208,960 + Pf6N2E2_1865 0.21 -0.3
2,208,960 + Pf6N2E2_1865 0.21 -0.6
2,208,973 + Pf6N2E2_1865 0.23 +0.3
2,208,973 + Pf6N2E2_1865 0.23 +0.3
2,209,301 + Pf6N2E2_1865 0.60 +0.3
2,209,318 + Pf6N2E2_1865 0.62 -0.9
2,209,319 - Pf6N2E2_1865 0.62 +0.3
2,209,319 - Pf6N2E2_1865 0.62 +0.3
2,209,455 + Pf6N2E2_1865 0.77 -0.7
2,209,513 + Pf6N2E2_1865 0.84 +0.3
2,209,513 + Pf6N2E2_1865 0.84 -0.5
2,209,514 - Pf6N2E2_1865 0.84 -0.7
2,209,514 - Pf6N2E2_1865 0.84 +0.3
2,209,592 + -0.7
2,209,592 + +0.3
2,209,711 - Pf6N2E2_1866 0.23 +0.3

Or see this region's nucleotide sequence