Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1849 and Pf6N2E2_1850 are separated by 228 nucleotides Pf6N2E2_1850 and Pf6N2E2_1851 are separated by 63 nucleotides
Pf6N2E2_1849 - FIG002188: hypothetical protein, at 2,188,255 to 2,189,619
_1849
Pf6N2E2_1850 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,189,848 to 2,191,995
_1850
Pf6N2E2_1851 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,192,059 to 2,193,267
_1851
Position (kb)
2189
2190
2191
2192 Strain fitness (log2 ratio)
-1
0
1 at 2189.069 kb on + strand, within Pf6N2E2_1849 at 2189.069 kb on + strand, within Pf6N2E2_1849 at 2189.169 kb on - strand, within Pf6N2E2_1849 at 2189.206 kb on - strand, within Pf6N2E2_1849 at 2189.386 kb on - strand, within Pf6N2E2_1849 at 2189.429 kb on + strand, within Pf6N2E2_1849 at 2189.463 kb on - strand, within Pf6N2E2_1849 at 2189.636 kb on - strand at 2189.637 kb on + strand at 2189.638 kb on - strand at 2189.753 kb on + strand at 2189.785 kb on + strand at 2189.786 kb on - strand at 2189.786 kb on - strand at 2189.790 kb on + strand at 2189.791 kb on - strand at 2189.916 kb on - strand at 2189.916 kb on - strand at 2189.964 kb on - strand at 2190.056 kb on - strand at 2190.056 kb on - strand at 2190.161 kb on + strand, within Pf6N2E2_1850 at 2190.161 kb on + strand, within Pf6N2E2_1850 at 2190.206 kb on + strand, within Pf6N2E2_1850 at 2190.206 kb on + strand, within Pf6N2E2_1850 at 2190.206 kb on + strand, within Pf6N2E2_1850 at 2190.207 kb on - strand, within Pf6N2E2_1850 at 2190.207 kb on - strand, within Pf6N2E2_1850 at 2190.242 kb on + strand, within Pf6N2E2_1850 at 2190.243 kb on - strand, within Pf6N2E2_1850 at 2190.337 kb on - strand, within Pf6N2E2_1850 at 2190.406 kb on + strand, within Pf6N2E2_1850 at 2190.406 kb on + strand, within Pf6N2E2_1850 at 2190.406 kb on + strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.407 kb on - strand, within Pf6N2E2_1850 at 2190.467 kb on + strand, within Pf6N2E2_1850 at 2190.825 kb on - strand, within Pf6N2E2_1850 at 2191.364 kb on - strand, within Pf6N2E2_1850 at 2191.635 kb on + strand, within Pf6N2E2_1850 at 2191.636 kb on - strand, within Pf6N2E2_1850 at 2191.870 kb on + strand at 2191.905 kb on - strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.996 kb on - strand at 2192.451 kb on + strand, within Pf6N2E2_1851 at 2192.505 kb on + strand, within Pf6N2E2_1851 at 2192.547 kb on - strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.551 kb on - strand, within Pf6N2E2_1851 at 2192.560 kb on + strand, within Pf6N2E2_1851 at 2192.561 kb on - strand, within Pf6N2E2_1851 at 2192.571 kb on - strand, within Pf6N2E2_1851 at 2192.581 kb on - strand, within Pf6N2E2_1851
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 2,189,069 + Pf6N2E2_1849 0.60 -1.2 2,189,069 + Pf6N2E2_1849 0.60 -0.7 2,189,169 - Pf6N2E2_1849 0.67 -0.7 2,189,206 - Pf6N2E2_1849 0.70 +0.3 2,189,386 - Pf6N2E2_1849 0.83 +0.3 2,189,429 + Pf6N2E2_1849 0.86 +0.3 2,189,463 - Pf6N2E2_1849 0.88 +0.3 2,189,636 - +0.0 2,189,637 + +0.3 2,189,638 - +0.3 2,189,753 + -0.7 2,189,785 + +0.3 2,189,786 - -0.6 2,189,786 - +0.3 2,189,790 + +0.3 2,189,791 - -0.7 2,189,916 - +0.3 2,189,916 - +0.7 2,189,964 - -1.3 2,190,056 - -0.6 2,190,056 - -0.7 2,190,161 + Pf6N2E2_1850 0.15 +0.4 2,190,161 + Pf6N2E2_1850 0.15 -0.7 2,190,206 + Pf6N2E2_1850 0.17 -1.3 2,190,206 + Pf6N2E2_1850 0.17 -0.1 2,190,206 + Pf6N2E2_1850 0.17 -0.7 2,190,207 - Pf6N2E2_1850 0.17 +0.3 2,190,207 - Pf6N2E2_1850 0.17 -0.7 2,190,242 + Pf6N2E2_1850 0.18 -0.7 2,190,243 - Pf6N2E2_1850 0.18 -1.3 2,190,337 - Pf6N2E2_1850 0.23 -0.7 2,190,406 + Pf6N2E2_1850 0.26 -0.7 2,190,406 + Pf6N2E2_1850 0.26 -0.7 2,190,406 + Pf6N2E2_1850 0.26 +0.3 2,190,407 - Pf6N2E2_1850 0.26 +0.3 2,190,407 - Pf6N2E2_1850 0.26 +0.3 2,190,407 - Pf6N2E2_1850 0.26 -0.7 2,190,407 - Pf6N2E2_1850 0.26 -0.8 2,190,407 - Pf6N2E2_1850 0.26 +0.3 2,190,407 - Pf6N2E2_1850 0.26 +0.3 2,190,467 + Pf6N2E2_1850 0.29 +0.3 2,190,825 - Pf6N2E2_1850 0.45 -0.7 2,191,364 - Pf6N2E2_1850 0.71 -1.1 2,191,635 + Pf6N2E2_1850 0.83 +0.3 2,191,636 - Pf6N2E2_1850 0.83 +0.3 2,191,870 + -0.7 2,191,905 - +0.3 2,191,995 + -0.7 2,191,995 + +0.3 2,191,995 + -0.6 2,191,995 + +0.3 2,191,995 + -0.7 2,191,996 - +0.3 2,192,451 + Pf6N2E2_1851 0.32 -1.3 2,192,505 + Pf6N2E2_1851 0.37 -1.3 2,192,547 - Pf6N2E2_1851 0.40 -1.0 2,192,550 + Pf6N2E2_1851 0.41 -0.7 2,192,550 + Pf6N2E2_1851 0.41 +0.3 2,192,550 + Pf6N2E2_1851 0.41 -0.4 2,192,551 - Pf6N2E2_1851 0.41 +0.3 2,192,560 + Pf6N2E2_1851 0.41 +0.3 2,192,561 - Pf6N2E2_1851 0.42 +0.3 2,192,571 - Pf6N2E2_1851 0.42 -1.3 2,192,581 - Pf6N2E2_1851 0.43 -0.5
Or see this region's nucleotide sequence