Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1267 and Pf6N2E2_1268 overlap by 33 nucleotides Pf6N2E2_1268 and Pf6N2E2_1269 are separated by 6 nucleotides Pf6N2E2_1269 and Pf6N2E2_1270 are separated by 294 nucleotides
Pf6N2E2_1267 - Membrane protein, putative, at 1,493,505 to 1,494,284
_1267
Pf6N2E2_1268 - NADH pyrophosphatase (EC 3.6.1.22), at 1,494,252 to 1,495,082
_1268
Pf6N2E2_1269 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,495,089 to 1,495,901
_1269
Pf6N2E2_1270 - Heavy metal RND efflux outer membrane protein, CzcC family, at 1,496,196 to 1,497,671
_1270
Position (kb)
1495
1496 Strain fitness (log2 ratio)
-2
-1
0
1 at 1494.283 kb on - strand at 1494.298 kb on + strand at 1494.298 kb on + strand at 1494.298 kb on + strand at 1494.299 kb on - strand at 1494.299 kb on - strand at 1494.478 kb on + strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.479 kb on - strand, within Pf6N2E2_1268 at 1494.635 kb on + strand, within Pf6N2E2_1268 at 1494.671 kb on - strand, within Pf6N2E2_1268 at 1494.697 kb on + strand, within Pf6N2E2_1268 at 1494.697 kb on + strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.698 kb on - strand, within Pf6N2E2_1268 at 1494.805 kb on + strand, within Pf6N2E2_1268 at 1494.806 kb on - strand, within Pf6N2E2_1268 at 1494.806 kb on - strand, within Pf6N2E2_1268 at 1494.895 kb on + strand, within Pf6N2E2_1268 at 1494.896 kb on - strand, within Pf6N2E2_1268 at 1495.122 kb on - strand at 1495.222 kb on + strand at 1495.222 kb on + strand, within Pf6N2E2_1269 at 1495.222 kb on + strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.223 kb on - strand, within Pf6N2E2_1269 at 1495.324 kb on + strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.325 kb on - strand, within Pf6N2E2_1269 at 1495.383 kb on - strand, within Pf6N2E2_1269 at 1495.385 kb on - strand, within Pf6N2E2_1269 at 1495.656 kb on + strand, within Pf6N2E2_1269 at 1495.656 kb on + strand, within Pf6N2E2_1269 at 1495.657 kb on - strand, within Pf6N2E2_1269 at 1495.704 kb on + strand, within Pf6N2E2_1269 at 1495.867 kb on - strand at 1495.892 kb on - strand at 1495.892 kb on - strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.939 kb on + strand at 1495.940 kb on - strand at 1495.940 kb on - strand at 1495.965 kb on - strand at 1496.166 kb on - strand at 1496.169 kb on - strand at 1496.342 kb on - strand at 1496.342 kb on - strand at 1496.342 kb on - strand at 1496.363 kb on - strand, within Pf6N2E2_1270 at 1496.459 kb on - strand, within Pf6N2E2_1270 at 1496.459 kb on - strand, within Pf6N2E2_1270 at 1496.500 kb on + strand, within Pf6N2E2_1270 at 1496.500 kb on + strand, within Pf6N2E2_1270 at 1496.521 kb on + strand, within Pf6N2E2_1270 at 1496.522 kb on - strand, within Pf6N2E2_1270 at 1496.535 kb on - strand, within Pf6N2E2_1270 at 1496.635 kb on + strand, within Pf6N2E2_1270 at 1496.636 kb on - strand, within Pf6N2E2_1270 at 1496.639 kb on + strand, within Pf6N2E2_1270 at 1496.639 kb on + strand, within Pf6N2E2_1270 at 1496.650 kb on + strand at 1496.686 kb on + strand, within Pf6N2E2_1270 at 1496.686 kb on + strand, within Pf6N2E2_1270
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 1,494,283 - -0.5 1,494,298 + +0.2 1,494,298 + +0.2 1,494,298 + -0.8 1,494,299 - +0.2 1,494,299 - +0.1 1,494,478 + Pf6N2E2_1268 0.27 +0.2 1,494,479 - Pf6N2E2_1268 0.27 -0.8 1,494,479 - Pf6N2E2_1268 0.27 +0.2 1,494,479 - Pf6N2E2_1268 0.27 +0.2 1,494,479 - Pf6N2E2_1268 0.27 +0.2 1,494,479 - Pf6N2E2_1268 0.27 -1.4 1,494,635 + Pf6N2E2_1268 0.46 -0.8 1,494,671 - Pf6N2E2_1268 0.50 +0.2 1,494,697 + Pf6N2E2_1268 0.54 -1.1 1,494,697 + Pf6N2E2_1268 0.54 +0.2 1,494,698 - Pf6N2E2_1268 0.54 -1.4 1,494,698 - Pf6N2E2_1268 0.54 -0.2 1,494,698 - Pf6N2E2_1268 0.54 -1.9 1,494,698 - Pf6N2E2_1268 0.54 +0.2 1,494,698 - Pf6N2E2_1268 0.54 +0.2 1,494,698 - Pf6N2E2_1268 0.54 -1.4 1,494,805 + Pf6N2E2_1268 0.67 +0.2 1,494,806 - Pf6N2E2_1268 0.67 +0.2 1,494,806 - Pf6N2E2_1268 0.67 +0.2 1,494,895 + Pf6N2E2_1268 0.77 +0.2 1,494,896 - Pf6N2E2_1268 0.77 -0.3 1,495,122 - +0.2 1,495,222 + +0.5 1,495,222 + Pf6N2E2_1269 0.16 +0.2 1,495,222 + Pf6N2E2_1269 0.16 -0.7 1,495,223 - Pf6N2E2_1269 0.16 +0.2 1,495,223 - Pf6N2E2_1269 0.16 +0.2 1,495,223 - Pf6N2E2_1269 0.16 +0.2 1,495,223 - Pf6N2E2_1269 0.16 +0.2 1,495,324 + Pf6N2E2_1269 0.29 -0.3 1,495,325 - Pf6N2E2_1269 0.29 +0.2 1,495,325 - Pf6N2E2_1269 0.29 -0.6 1,495,325 - Pf6N2E2_1269 0.29 +0.2 1,495,383 - Pf6N2E2_1269 0.36 -2.1 1,495,385 - Pf6N2E2_1269 0.36 +0.2 1,495,656 + Pf6N2E2_1269 0.70 +0.2 1,495,656 + Pf6N2E2_1269 0.70 +0.2 1,495,657 - Pf6N2E2_1269 0.70 +0.2 1,495,704 + Pf6N2E2_1269 0.76 +0.2 1,495,867 - -1.4 1,495,892 - +0.2 1,495,892 - +0.2 1,495,939 + +0.2 1,495,939 + -0.2 1,495,939 + +0.2 1,495,939 + +0.2 1,495,940 - +1.2 1,495,940 - +0.2 1,495,965 - +0.2 1,496,166 - -0.5 1,496,169 - +0.2 1,496,342 - -0.8 1,496,342 - +0.2 1,496,342 - +0.2 1,496,363 - Pf6N2E2_1270 0.11 +0.2 1,496,459 - Pf6N2E2_1270 0.18 +0.2 1,496,459 - Pf6N2E2_1270 0.18 -1.8 1,496,500 + Pf6N2E2_1270 0.21 +0.2 1,496,500 + Pf6N2E2_1270 0.21 -0.8 1,496,521 + Pf6N2E2_1270 0.22 -0.8 1,496,522 - Pf6N2E2_1270 0.22 +0.2 1,496,535 - Pf6N2E2_1270 0.23 +0.2 1,496,635 + Pf6N2E2_1270 0.30 +0.6 1,496,636 - Pf6N2E2_1270 0.30 +0.2 1,496,639 + Pf6N2E2_1270 0.30 +0.2 1,496,639 + Pf6N2E2_1270 0.30 +0.2 1,496,650 + +0.2 1,496,686 + Pf6N2E2_1270 0.33 -2.6 1,496,686 + Pf6N2E2_1270 0.33 -0.5
Or see this region's nucleotide sequence