Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1245 and Pf6N2E2_1246 are separated by 25 nucleotides Pf6N2E2_1246 and Pf6N2E2_1247 are separated by 88 nucleotides Pf6N2E2_1247 and Pf6N2E2_1248 are separated by 285 nucleotides Pf6N2E2_1248 and Pf6N2E2_1249 are separated by 145 nucleotides
Pf6N2E2_1245 - hypothetical protein, at 1,458,496 to 1,458,921
_1245
Pf6N2E2_1246 - FIG00961372: hypothetical protein, at 1,458,947 to 1,459,174
_1246
Pf6N2E2_1247 - hypothetical protein, at 1,459,263 to 1,459,448
_1247
Pf6N2E2_1248 - MutT/nudix family protein, at 1,459,734 to 1,460,096
_1248
Pf6N2E2_1249 - Transcriptional regulator, AraC family, at 1,460,242 to 1,461,165
_1249
Position (kb)
1459
1460
1461 Strain fitness (log2 ratio)
-2
-1
0
1 at 1458.844 kb on + strand, within Pf6N2E2_1245 at 1458.845 kb on - strand, within Pf6N2E2_1245 at 1458.845 kb on - strand, within Pf6N2E2_1245 at 1458.847 kb on + strand, within Pf6N2E2_1245 at 1458.848 kb on - strand, within Pf6N2E2_1245 at 1458.848 kb on - strand, within Pf6N2E2_1245 at 1458.848 kb on - strand, within Pf6N2E2_1245 at 1458.850 kb on + strand, within Pf6N2E2_1245 at 1459.025 kb on + strand, within Pf6N2E2_1246 at 1459.026 kb on - strand, within Pf6N2E2_1246 at 1459.026 kb on - strand, within Pf6N2E2_1246 at 1459.155 kb on + strand at 1459.155 kb on + strand at 1459.204 kb on - strand at 1459.282 kb on + strand, within Pf6N2E2_1247 at 1459.282 kb on + strand, within Pf6N2E2_1247 at 1459.301 kb on - strand, within Pf6N2E2_1247 at 1459.301 kb on - strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.362 kb on + strand, within Pf6N2E2_1247 at 1459.363 kb on - strand, within Pf6N2E2_1247 at 1459.547 kb on - strand at 1459.547 kb on - strand at 1459.553 kb on - strand at 1459.593 kb on + strand at 1459.594 kb on - strand at 1459.594 kb on - strand at 1459.632 kb on + strand at 1459.652 kb on + strand at 1459.694 kb on + strand at 1459.694 kb on + strand at 1459.695 kb on - strand at 1459.695 kb on - strand at 1459.696 kb on + strand at 1459.696 kb on + strand at 1459.696 kb on + strand at 1459.697 kb on - strand at 1459.706 kb on + strand at 1459.706 kb on + strand at 1459.859 kb on + strand, within Pf6N2E2_1248 at 1459.860 kb on - strand, within Pf6N2E2_1248 at 1459.919 kb on + strand, within Pf6N2E2_1248 at 1459.919 kb on + strand, within Pf6N2E2_1248 at 1459.919 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.094 kb on + strand at 1460.124 kb on - strand at 1460.166 kb on - strand at 1460.220 kb on + strand at 1460.221 kb on - strand at 1460.221 kb on - strand at 1460.399 kb on + strand, within Pf6N2E2_1249 at 1460.400 kb on - strand, within Pf6N2E2_1249 at 1460.432 kb on + strand, within Pf6N2E2_1249 at 1460.495 kb on + strand, within Pf6N2E2_1249 at 1460.552 kb on + strand, within Pf6N2E2_1249 at 1460.552 kb on + strand, within Pf6N2E2_1249 at 1460.634 kb on + strand, within Pf6N2E2_1249 at 1460.635 kb on - strand, within Pf6N2E2_1249 at 1460.963 kb on + strand, within Pf6N2E2_1249
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 1,458,844 + Pf6N2E2_1245 0.82 +0.2 1,458,845 - Pf6N2E2_1245 0.82 +0.2 1,458,845 - Pf6N2E2_1245 0.82 +0.2 1,458,847 + Pf6N2E2_1245 0.82 -0.8 1,458,848 - Pf6N2E2_1245 0.83 +0.2 1,458,848 - Pf6N2E2_1245 0.83 -1.4 1,458,848 - Pf6N2E2_1245 0.83 +0.2 1,458,850 + Pf6N2E2_1245 0.83 +0.2 1,459,025 + Pf6N2E2_1246 0.34 +0.2 1,459,026 - Pf6N2E2_1246 0.35 +0.2 1,459,026 - Pf6N2E2_1246 0.35 -0.8 1,459,155 + +0.2 1,459,155 + +0.2 1,459,204 - +0.2 1,459,282 + Pf6N2E2_1247 0.10 -0.8 1,459,282 + Pf6N2E2_1247 0.10 +0.2 1,459,301 - Pf6N2E2_1247 0.20 -0.6 1,459,301 - Pf6N2E2_1247 0.20 +0.2 1,459,362 + Pf6N2E2_1247 0.53 +0.2 1,459,362 + Pf6N2E2_1247 0.53 -0.8 1,459,362 + Pf6N2E2_1247 0.53 +0.2 1,459,362 + Pf6N2E2_1247 0.53 +0.2 1,459,362 + Pf6N2E2_1247 0.53 +0.2 1,459,363 - Pf6N2E2_1247 0.54 +0.2 1,459,547 - -0.6 1,459,547 - +0.4 1,459,553 - -1.1 1,459,593 + -1.1 1,459,594 - -0.8 1,459,594 - +0.2 1,459,632 + -1.8 1,459,652 + +0.2 1,459,694 + +0.2 1,459,694 + +0.2 1,459,695 - +0.2 1,459,695 - -0.8 1,459,696 + +0.2 1,459,696 + -0.8 1,459,696 + +0.2 1,459,697 - -0.9 1,459,706 + -0.8 1,459,706 + +0.8 1,459,859 + Pf6N2E2_1248 0.34 +0.2 1,459,860 - Pf6N2E2_1248 0.35 -0.8 1,459,919 + Pf6N2E2_1248 0.51 -0.8 1,459,919 + Pf6N2E2_1248 0.51 -0.8 1,459,919 + +0.2 1,460,094 + -1.1 1,460,094 + +0.2 1,460,094 + -1.8 1,460,094 + -0.8 1,460,094 + +0.5 1,460,094 + +0.2 1,460,124 - +0.2 1,460,166 - +0.2 1,460,220 + -0.8 1,460,221 - +0.2 1,460,221 - +0.2 1,460,399 + Pf6N2E2_1249 0.17 -0.8 1,460,400 - Pf6N2E2_1249 0.17 +0.2 1,460,432 + Pf6N2E2_1249 0.21 -0.8 1,460,495 + Pf6N2E2_1249 0.27 -0.8 1,460,552 + Pf6N2E2_1249 0.34 -0.8 1,460,552 + Pf6N2E2_1249 0.34 +0.2 1,460,634 + Pf6N2E2_1249 0.42 -0.4 1,460,635 - Pf6N2E2_1249 0.43 +0.2 1,460,963 + Pf6N2E2_1249 0.78 -0.8
Or see this region's nucleotide sequence