Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1149

Experiment: Ying_AminoAcid20 rep C; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1148 and Pf6N2E2_1149 are separated by 3 nucleotidesPf6N2E2_1149 and Pf6N2E2_1150 are separated by 134 nucleotides Pf6N2E2_1148 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,345,431 to 1,346,582 _1148 Pf6N2E2_1149 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4), at 1,346,586 to 1,347,692 _1149 Pf6N2E2_1150 - CBS-domain-containing membrane protein, at 1,347,827 to 1,348,534 _1150 Position (kb) 1346 1347 1348Strain fitness (log2 ratio) -1 0 1 2at 1345.789 kb on + strand, within Pf6N2E2_1148at 1346.012 kb on + strand, within Pf6N2E2_1148at 1346.392 kb on + strand, within Pf6N2E2_1148at 1346.393 kb on - strand, within Pf6N2E2_1148at 1346.393 kb on - strand, within Pf6N2E2_1148at 1346.393 kb on - strand, within Pf6N2E2_1148at 1346.396 kb on + strand, within Pf6N2E2_1148at 1346.418 kb on + strand, within Pf6N2E2_1148at 1346.703 kb on + strand, within Pf6N2E2_1149at 1346.703 kb on + strand, within Pf6N2E2_1149at 1346.704 kb on - strand, within Pf6N2E2_1149at 1346.704 kb on - strand, within Pf6N2E2_1149at 1346.862 kb on + strand, within Pf6N2E2_1149at 1346.863 kb on - strand, within Pf6N2E2_1149at 1347.038 kb on + strand, within Pf6N2E2_1149at 1347.038 kb on + strand, within Pf6N2E2_1149at 1347.121 kb on + strand, within Pf6N2E2_1149at 1347.121 kb on + strand, within Pf6N2E2_1149at 1347.122 kb on - strand, within Pf6N2E2_1149at 1347.122 kb on - strand, within Pf6N2E2_1149at 1347.122 kb on - strand, within Pf6N2E2_1149at 1347.122 kb on - strand, within Pf6N2E2_1149at 1347.122 kb on - strand, within Pf6N2E2_1149at 1347.166 kb on + strand, within Pf6N2E2_1149at 1347.166 kb on + strand, within Pf6N2E2_1149at 1347.167 kb on - strand, within Pf6N2E2_1149at 1347.167 kb on - strand, within Pf6N2E2_1149at 1347.202 kb on + strand, within Pf6N2E2_1149at 1347.436 kb on + strand, within Pf6N2E2_1149at 1347.437 kb on - strand, within Pf6N2E2_1149at 1347.738 kb on + strandat 1347.739 kb on - strandat 1347.807 kb on + strandat 1347.807 kb on + strandat 1347.808 kb on - strandat 1347.811 kb on + strandat 1347.811 kb on + strandat 1347.812 kb on - strandat 1347.812 kb on - strandat 1347.812 kb on - strandat 1347.812 kb on - strandat 1347.812 kb on - strandat 1347.812 kb on - strandat 1348.063 kb on + strand, within Pf6N2E2_1150at 1348.063 kb on + strand, within Pf6N2E2_1150at 1348.065 kb on + strand, within Pf6N2E2_1150at 1348.065 kb on + strand, within Pf6N2E2_1150at 1348.065 kb on + strand, within Pf6N2E2_1150at 1348.141 kb on + strand, within Pf6N2E2_1150at 1348.268 kb on + strand, within Pf6N2E2_1150at 1348.268 kb on + strand, within Pf6N2E2_1150at 1348.269 kb on - strand, within Pf6N2E2_1150at 1348.269 kb on - strand, within Pf6N2E2_1150at 1348.269 kb on - strand, within Pf6N2E2_1150at 1348.669 kb on + strandat 1348.670 kb on - strandat 1348.670 kb on - strandat 1348.670 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3
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1,345,789 + Pf6N2E2_1148 0.31 +0.3
1,346,012 + Pf6N2E2_1148 0.50 +0.3
1,346,392 + Pf6N2E2_1148 0.83 +0.3
1,346,393 - Pf6N2E2_1148 0.84 +0.3
1,346,393 - Pf6N2E2_1148 0.84 -1.2
1,346,393 - Pf6N2E2_1148 0.84 -0.7
1,346,396 + Pf6N2E2_1148 0.84 -0.7
1,346,418 + Pf6N2E2_1148 0.86 +0.3
1,346,703 + Pf6N2E2_1149 0.11 +0.3
1,346,703 + Pf6N2E2_1149 0.11 -0.7
1,346,704 - Pf6N2E2_1149 0.11 -0.7
1,346,704 - Pf6N2E2_1149 0.11 -0.1
1,346,862 + Pf6N2E2_1149 0.25 +0.3
1,346,863 - Pf6N2E2_1149 0.25 -0.7
1,347,038 + Pf6N2E2_1149 0.41 -0.4
1,347,038 + Pf6N2E2_1149 0.41 -0.0
1,347,121 + Pf6N2E2_1149 0.48 +0.3
1,347,121 + Pf6N2E2_1149 0.48 -1.6
1,347,122 - Pf6N2E2_1149 0.48 +1.0
1,347,122 - Pf6N2E2_1149 0.48 +0.3
1,347,122 - Pf6N2E2_1149 0.48 +1.8
1,347,122 - Pf6N2E2_1149 0.48 +0.3
1,347,122 - Pf6N2E2_1149 0.48 +0.3
1,347,166 + Pf6N2E2_1149 0.52 -0.0
1,347,166 + Pf6N2E2_1149 0.52 +0.3
1,347,167 - Pf6N2E2_1149 0.52 -0.7
1,347,167 - Pf6N2E2_1149 0.52 +0.3
1,347,202 + Pf6N2E2_1149 0.56 +0.3
1,347,436 + Pf6N2E2_1149 0.77 +0.3
1,347,437 - Pf6N2E2_1149 0.77 +0.3
1,347,738 + +0.3
1,347,739 - +0.3
1,347,807 + +0.3
1,347,807 + +0.3
1,347,808 - -0.2
1,347,811 + +0.3
1,347,811 + -0.7
1,347,812 - +0.3
1,347,812 - -0.7
1,347,812 - -1.3
1,347,812 - -1.0
1,347,812 - -0.6
1,347,812 - +0.3
1,348,063 + Pf6N2E2_1150 0.33 +0.3
1,348,063 + Pf6N2E2_1150 0.33 -0.7
1,348,065 + Pf6N2E2_1150 0.34 +0.7
1,348,065 + Pf6N2E2_1150 0.34 +0.3
1,348,065 + Pf6N2E2_1150 0.34 +0.3
1,348,141 + Pf6N2E2_1150 0.44 -0.8
1,348,268 + Pf6N2E2_1150 0.62 -0.6
1,348,268 + Pf6N2E2_1150 0.62 +0.3
1,348,269 - Pf6N2E2_1150 0.62 +0.3
1,348,269 - Pf6N2E2_1150 0.62 +0.3
1,348,269 - Pf6N2E2_1150 0.62 +0.3
1,348,669 + -0.5
1,348,670 - +0.3
1,348,670 - -0.4
1,348,670 - -0.3

Or see this region's nucleotide sequence