Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1148 and Pf6N2E2_1149 are separated by 3 nucleotides Pf6N2E2_1149 and Pf6N2E2_1150 are separated by 134 nucleotides
Pf6N2E2_1148 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,345,431 to 1,346,582
_1148
Pf6N2E2_1149 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4), at 1,346,586 to 1,347,692
_1149
Pf6N2E2_1150 - CBS-domain-containing membrane protein, at 1,347,827 to 1,348,534
_1150
Position (kb)
1346
1347
1348 Strain fitness (log2 ratio)
-1
0
1
2 at 1345.789 kb on + strand, within Pf6N2E2_1148 at 1346.012 kb on + strand, within Pf6N2E2_1148 at 1346.392 kb on + strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.393 kb on - strand, within Pf6N2E2_1148 at 1346.396 kb on + strand, within Pf6N2E2_1148 at 1346.418 kb on + strand, within Pf6N2E2_1148 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.703 kb on + strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.704 kb on - strand, within Pf6N2E2_1149 at 1346.862 kb on + strand, within Pf6N2E2_1149 at 1346.863 kb on - strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.038 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.121 kb on + strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.122 kb on - strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.166 kb on + strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.167 kb on - strand, within Pf6N2E2_1149 at 1347.202 kb on + strand, within Pf6N2E2_1149 at 1347.436 kb on + strand, within Pf6N2E2_1149 at 1347.437 kb on - strand, within Pf6N2E2_1149 at 1347.738 kb on + strand at 1347.739 kb on - strand at 1347.807 kb on + strand at 1347.807 kb on + strand at 1347.808 kb on - strand at 1347.811 kb on + strand at 1347.811 kb on + strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1347.812 kb on - strand at 1348.063 kb on + strand, within Pf6N2E2_1150 at 1348.063 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.065 kb on + strand, within Pf6N2E2_1150 at 1348.141 kb on + strand, within Pf6N2E2_1150 at 1348.268 kb on + strand, within Pf6N2E2_1150 at 1348.268 kb on + strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.269 kb on - strand, within Pf6N2E2_1150 at 1348.669 kb on + strand at 1348.670 kb on - strand at 1348.670 kb on - strand at 1348.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 1,345,789 + Pf6N2E2_1148 0.31 +0.3 1,346,012 + Pf6N2E2_1148 0.50 +0.3 1,346,392 + Pf6N2E2_1148 0.83 +0.3 1,346,393 - Pf6N2E2_1148 0.84 +0.3 1,346,393 - Pf6N2E2_1148 0.84 -1.2 1,346,393 - Pf6N2E2_1148 0.84 -0.7 1,346,396 + Pf6N2E2_1148 0.84 -0.7 1,346,418 + Pf6N2E2_1148 0.86 +0.3 1,346,703 + Pf6N2E2_1149 0.11 +0.3 1,346,703 + Pf6N2E2_1149 0.11 -0.7 1,346,704 - Pf6N2E2_1149 0.11 -0.7 1,346,704 - Pf6N2E2_1149 0.11 -0.1 1,346,862 + Pf6N2E2_1149 0.25 +0.3 1,346,863 - Pf6N2E2_1149 0.25 -0.7 1,347,038 + Pf6N2E2_1149 0.41 -0.4 1,347,038 + Pf6N2E2_1149 0.41 -0.0 1,347,121 + Pf6N2E2_1149 0.48 +0.3 1,347,121 + Pf6N2E2_1149 0.48 -1.6 1,347,122 - Pf6N2E2_1149 0.48 +1.0 1,347,122 - Pf6N2E2_1149 0.48 +0.3 1,347,122 - Pf6N2E2_1149 0.48 +1.8 1,347,122 - Pf6N2E2_1149 0.48 +0.3 1,347,122 - Pf6N2E2_1149 0.48 +0.3 1,347,166 + Pf6N2E2_1149 0.52 -0.0 1,347,166 + Pf6N2E2_1149 0.52 +0.3 1,347,167 - Pf6N2E2_1149 0.52 -0.7 1,347,167 - Pf6N2E2_1149 0.52 +0.3 1,347,202 + Pf6N2E2_1149 0.56 +0.3 1,347,436 + Pf6N2E2_1149 0.77 +0.3 1,347,437 - Pf6N2E2_1149 0.77 +0.3 1,347,738 + +0.3 1,347,739 - +0.3 1,347,807 + +0.3 1,347,807 + +0.3 1,347,808 - -0.2 1,347,811 + +0.3 1,347,811 + -0.7 1,347,812 - +0.3 1,347,812 - -0.7 1,347,812 - -1.3 1,347,812 - -1.0 1,347,812 - -0.6 1,347,812 - +0.3 1,348,063 + Pf6N2E2_1150 0.33 +0.3 1,348,063 + Pf6N2E2_1150 0.33 -0.7 1,348,065 + Pf6N2E2_1150 0.34 +0.7 1,348,065 + Pf6N2E2_1150 0.34 +0.3 1,348,065 + Pf6N2E2_1150 0.34 +0.3 1,348,141 + Pf6N2E2_1150 0.44 -0.8 1,348,268 + Pf6N2E2_1150 0.62 -0.6 1,348,268 + Pf6N2E2_1150 0.62 +0.3 1,348,269 - Pf6N2E2_1150 0.62 +0.3 1,348,269 - Pf6N2E2_1150 0.62 +0.3 1,348,269 - Pf6N2E2_1150 0.62 +0.3 1,348,669 + -0.5 1,348,670 - +0.3 1,348,670 - -0.4 1,348,670 - -0.3
Or see this region's nucleotide sequence