Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1145 and Pf6N2E2_1146 are separated by 9 nucleotides Pf6N2E2_1146 and Pf6N2E2_1147 are separated by 13 nucleotides
Pf6N2E2_1145 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16), at 1,342,085 to 1,343,275
_1145
Pf6N2E2_1146 - 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 1,343,285 to 1,344,412
_1146
Pf6N2E2_1147 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17), at 1,344,426 to 1,345,199
_1147
Position (kb)
1343
1344
1345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1343.270 kb on + strand at 1343.270 kb on + strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.271 kb on - strand at 1343.273 kb on + strand at 1343.281 kb on + strand at 1343.281 kb on + strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.282 kb on - strand at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.483 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.720 kb on + strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.721 kb on - strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.972 kb on + strand, within Pf6N2E2_1146 at 1343.973 kb on - strand, within Pf6N2E2_1146 at 1344.068 kb on + strand, within Pf6N2E2_1146 at 1344.069 kb on - strand, within Pf6N2E2_1146 at 1344.179 kb on + strand, within Pf6N2E2_1146 at 1344.362 kb on + strand at 1344.362 kb on + strand at 1344.362 kb on + strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.363 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.433 kb on - strand at 1344.660 kb on + strand, within Pf6N2E2_1147 at 1344.661 kb on - strand, within Pf6N2E2_1147 at 1345.031 kb on + strand, within Pf6N2E2_1147 at 1345.032 kb on - strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.193 kb on + strand at 1345.194 kb on - strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.197 kb on + strand at 1345.198 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 1,343,270 + +0.3 1,343,270 + +0.3 1,343,271 - -0.7 1,343,271 - -0.7 1,343,271 - +0.3 1,343,273 + +0.3 1,343,281 + +0.3 1,343,281 + -2.1 1,343,282 - -0.7 1,343,282 - +0.3 1,343,282 - -0.7 1,343,483 + Pf6N2E2_1146 0.18 +0.3 1,343,483 + Pf6N2E2_1146 0.18 +0.3 1,343,720 + Pf6N2E2_1146 0.39 -0.7 1,343,720 + Pf6N2E2_1146 0.39 +0.3 1,343,720 + Pf6N2E2_1146 0.39 -0.7 1,343,720 + Pf6N2E2_1146 0.39 +0.3 1,343,720 + Pf6N2E2_1146 0.39 +0.3 1,343,721 - Pf6N2E2_1146 0.39 -0.4 1,343,721 - Pf6N2E2_1146 0.39 +0.3 1,343,972 + Pf6N2E2_1146 0.61 +0.3 1,343,972 + Pf6N2E2_1146 0.61 -0.7 1,343,973 - Pf6N2E2_1146 0.61 +0.3 1,344,068 + Pf6N2E2_1146 0.69 +0.3 1,344,069 - Pf6N2E2_1146 0.70 +1.3 1,344,179 + Pf6N2E2_1146 0.79 +0.3 1,344,362 + +0.3 1,344,362 + +0.3 1,344,362 + +1.8 1,344,363 - -1.3 1,344,363 - -1.7 1,344,363 - -0.7 1,344,363 - +0.3 1,344,363 - -0.7 1,344,433 - -0.7 1,344,433 - -2.3 1,344,433 - -0.7 1,344,660 + Pf6N2E2_1147 0.30 -2.8 1,344,661 - Pf6N2E2_1147 0.30 +0.3 1,345,031 + Pf6N2E2_1147 0.78 +0.3 1,345,032 - +0.3 1,345,193 + -0.7 1,345,193 + +0.3 1,345,193 + +0.3 1,345,194 - +0.3 1,345,197 + -0.7 1,345,197 + -0.7 1,345,197 + -1.5 1,345,197 + +0.3 1,345,198 - +0.3
Or see this region's nucleotide sequence