Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1045 and Pf6N2E2_1046 are separated by 624 nucleotides Pf6N2E2_1046 and Pf6N2E2_1047 are separated by 25 nucleotides
Pf6N2E2_1045 - hypothetical protein, at 1,219,379 to 1,219,516
_1045
Pf6N2E2_1046 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,220,141 to 1,220,962
_1046
Pf6N2E2_1047 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,220,988 to 1,221,992
_1047
Position (kb)
1220
1221 Strain fitness (log2 ratio)
-2
-1
0
1 at 1219.179 kb on + strand at 1219.329 kb on - strand at 1219.514 kb on + strand at 1219.515 kb on - strand at 1219.515 kb on - strand at 1219.515 kb on - strand at 1219.562 kb on - strand at 1219.572 kb on + strand at 1219.573 kb on - strand at 1219.587 kb on - strand at 1219.640 kb on + strand at 1219.641 kb on - strand at 1219.641 kb on - strand at 1219.658 kb on + strand at 1219.715 kb on + strand at 1219.782 kb on + strand at 1219.783 kb on - strand at 1219.783 kb on - strand at 1219.789 kb on + strand at 1219.789 kb on + strand at 1219.790 kb on - strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.820 kb on - strand at 1219.820 kb on - strand at 1219.909 kb on - strand at 1219.968 kb on + strand at 1219.968 kb on + strand at 1220.001 kb on - strand at 1220.067 kb on + strand at 1220.067 kb on + strand at 1220.067 kb on + strand at 1220.067 kb on + strand at 1220.068 kb on - strand at 1220.121 kb on + strand at 1220.122 kb on - strand at 1220.122 kb on - strand at 1220.157 kb on + strand at 1220.211 kb on + strand at 1220.212 kb on - strand at 1220.388 kb on + strand, within Pf6N2E2_1046 at 1220.389 kb on - strand, within Pf6N2E2_1046 at 1220.434 kb on - strand, within Pf6N2E2_1046 at 1220.642 kb on - strand, within Pf6N2E2_1046 at 1220.737 kb on + strand, within Pf6N2E2_1046 at 1220.781 kb on + strand, within Pf6N2E2_1046 at 1220.782 kb on - strand, within Pf6N2E2_1046 at 1220.811 kb on - strand, within Pf6N2E2_1046 at 1221.053 kb on - strand at 1221.101 kb on + strand, within Pf6N2E2_1047 at 1221.208 kb on + strand, within Pf6N2E2_1047 at 1221.209 kb on - strand, within Pf6N2E2_1047 at 1221.209 kb on - strand, within Pf6N2E2_1047 at 1221.300 kb on + strand, within Pf6N2E2_1047 at 1221.300 kb on + strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.301 kb on - strand, within Pf6N2E2_1047 at 1221.367 kb on + strand, within Pf6N2E2_1047 at 1221.367 kb on + strand, within Pf6N2E2_1047 at 1221.368 kb on - strand, within Pf6N2E2_1047 at 1221.368 kb on - strand, within Pf6N2E2_1047 at 1221.368 kb on - strand, within Pf6N2E2_1047 at 1221.733 kb on + strand, within Pf6N2E2_1047
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 1,219,179 + -0.6 1,219,329 - +0.3 1,219,514 + +1.3 1,219,515 - -0.7 1,219,515 - +1.0 1,219,515 - +0.6 1,219,562 - -1.1 1,219,572 + +0.3 1,219,573 - -0.7 1,219,587 - +0.3 1,219,640 + -2.7 1,219,641 - -0.7 1,219,641 - +0.3 1,219,658 + +0.3 1,219,715 + +0.3 1,219,782 + -0.8 1,219,783 - -0.8 1,219,783 - +0.3 1,219,789 + -1.7 1,219,789 + +0.3 1,219,790 - -0.7 1,219,819 + +0.3 1,219,819 + +0.3 1,219,819 + +0.3 1,219,819 + +0.3 1,219,820 - +0.3 1,219,820 - -0.3 1,219,909 - -1.3 1,219,968 + -0.9 1,219,968 + +0.3 1,220,001 - -1.4 1,220,067 + +0.3 1,220,067 + -1.3 1,220,067 + +0.3 1,220,067 + +0.3 1,220,068 - +0.3 1,220,121 + +0.3 1,220,122 - +0.3 1,220,122 - +0.3 1,220,157 + -0.5 1,220,211 + +0.3 1,220,212 - -0.7 1,220,388 + Pf6N2E2_1046 0.30 +0.3 1,220,389 - Pf6N2E2_1046 0.30 -0.7 1,220,434 - Pf6N2E2_1046 0.36 -0.7 1,220,642 - Pf6N2E2_1046 0.61 +0.3 1,220,737 + Pf6N2E2_1046 0.73 -0.2 1,220,781 + Pf6N2E2_1046 0.78 -0.7 1,220,782 - Pf6N2E2_1046 0.78 +0.3 1,220,811 - Pf6N2E2_1046 0.82 +0.3 1,221,053 - +0.3 1,221,101 + Pf6N2E2_1047 0.11 +0.3 1,221,208 + Pf6N2E2_1047 0.22 +0.3 1,221,209 - Pf6N2E2_1047 0.22 +0.3 1,221,209 - Pf6N2E2_1047 0.22 -1.3 1,221,300 + Pf6N2E2_1047 0.31 +0.3 1,221,300 + Pf6N2E2_1047 0.31 +0.3 1,221,301 - Pf6N2E2_1047 0.31 +0.3 1,221,301 - Pf6N2E2_1047 0.31 -1.0 1,221,301 - Pf6N2E2_1047 0.31 -0.7 1,221,301 - Pf6N2E2_1047 0.31 -0.1 1,221,367 + Pf6N2E2_1047 0.38 -0.7 1,221,367 + Pf6N2E2_1047 0.38 -0.7 1,221,368 - Pf6N2E2_1047 0.38 -0.5 1,221,368 - Pf6N2E2_1047 0.38 +0.3 1,221,368 - Pf6N2E2_1047 0.38 -0.2 1,221,733 + Pf6N2E2_1047 0.74 +0.3
Or see this region's nucleotide sequence