Experiment: Ying_AminoAcid20 rep C; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotides Pf6N2E2_1022 and Pf6N2E2_1023 are separated by 50 nucleotides
Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826
_1021
Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022
_1022
Pf6N2E2_1023 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,196,073 to 1,197,065
_1023
Position (kb)
1194
1195
1196
1197 Strain fitness (log2 ratio)
-2
-1
0
1 at 1193.883 kb on + strand at 1193.895 kb on - strand at 1193.895 kb on - strand at 1193.998 kb on + strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.459 kb on - strand, within Pf6N2E2_1021 at 1194.583 kb on - strand, within Pf6N2E2_1021 at 1194.628 kb on - strand, within Pf6N2E2_1021 at 1195.244 kb on + strand, within Pf6N2E2_1022 at 1195.366 kb on + strand, within Pf6N2E2_1022 at 1195.396 kb on + strand, within Pf6N2E2_1022 at 1195.733 kb on + strand, within Pf6N2E2_1022 at 1195.913 kb on + strand at 1195.913 kb on + strand at 1195.914 kb on - strand at 1195.914 kb on - strand at 1195.914 kb on - strand at 1195.914 kb on - strand at 1196.005 kb on + strand at 1196.005 kb on + strand at 1196.006 kb on - strand at 1196.046 kb on + strand at 1196.057 kb on + strand at 1196.057 kb on + strand at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.407 kb on + strand, within Pf6N2E2_1023 at 1196.407 kb on + strand, within Pf6N2E2_1023 at 1196.408 kb on - strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.489 kb on - strand, within Pf6N2E2_1023 at 1196.869 kb on + strand, within Pf6N2E2_1023 at 1196.923 kb on + strand, within Pf6N2E2_1023
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep C; time point 3 remove 1,193,883 + +0.3 1,193,895 - +0.3 1,193,895 - +0.3 1,193,998 + Pf6N2E2_1021 0.13 -0.9 1,193,999 - Pf6N2E2_1021 0.13 +0.3 1,193,999 - Pf6N2E2_1021 0.13 -0.9 1,194,030 + Pf6N2E2_1021 0.16 +0.3 1,194,030 + Pf6N2E2_1021 0.16 -1.3 1,194,030 + Pf6N2E2_1021 0.16 +0.3 1,194,031 - Pf6N2E2_1021 0.16 +0.3 1,194,031 - Pf6N2E2_1021 0.16 -0.7 1,194,409 + Pf6N2E2_1021 0.56 -0.4 1,194,409 + Pf6N2E2_1021 0.56 -0.3 1,194,409 + Pf6N2E2_1021 0.56 -0.7 1,194,410 - Pf6N2E2_1021 0.56 +0.3 1,194,410 - Pf6N2E2_1021 0.56 +0.3 1,194,410 - Pf6N2E2_1021 0.56 +0.3 1,194,459 - Pf6N2E2_1021 0.61 -0.7 1,194,583 - Pf6N2E2_1021 0.74 -0.7 1,194,628 - Pf6N2E2_1021 0.79 +0.3 1,195,244 + Pf6N2E2_1022 0.34 +0.3 1,195,366 + Pf6N2E2_1022 0.45 -0.6 1,195,396 + Pf6N2E2_1022 0.47 -0.3 1,195,733 + Pf6N2E2_1022 0.76 +0.3 1,195,913 + -1.7 1,195,913 + +0.3 1,195,914 - -1.0 1,195,914 - +0.3 1,195,914 - +0.3 1,195,914 - +0.3 1,196,005 + +0.3 1,196,005 + +0.3 1,196,006 - +0.3 1,196,046 + -0.8 1,196,057 + +0.3 1,196,057 + -1.8 1,196,233 + Pf6N2E2_1023 0.16 -0.3 1,196,233 + Pf6N2E2_1023 0.16 +1.3 1,196,233 + Pf6N2E2_1023 0.16 -0.7 1,196,233 + Pf6N2E2_1023 0.16 +0.3 1,196,233 + Pf6N2E2_1023 0.16 -0.7 1,196,233 + Pf6N2E2_1023 0.16 -0.5 1,196,233 + Pf6N2E2_1023 0.16 +0.3 1,196,234 - Pf6N2E2_1023 0.16 -0.7 1,196,234 - Pf6N2E2_1023 0.16 -0.7 1,196,234 - Pf6N2E2_1023 0.16 +0.3 1,196,234 - Pf6N2E2_1023 0.16 -0.7 1,196,344 + Pf6N2E2_1023 0.27 +0.6 1,196,344 + Pf6N2E2_1023 0.27 +0.3 1,196,344 + Pf6N2E2_1023 0.27 -0.3 1,196,407 + Pf6N2E2_1023 0.34 +0.3 1,196,407 + Pf6N2E2_1023 0.34 +0.3 1,196,408 - Pf6N2E2_1023 0.34 -2.0 1,196,488 + Pf6N2E2_1023 0.42 -0.1 1,196,488 + Pf6N2E2_1023 0.42 +0.3 1,196,488 + Pf6N2E2_1023 0.42 +0.3 1,196,489 - Pf6N2E2_1023 0.42 +0.3 1,196,869 + Pf6N2E2_1023 0.80 -0.7 1,196,923 + Pf6N2E2_1023 0.86 +0.3
Or see this region's nucleotide sequence