Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_670

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_668 and Pf6N2E2_669 are separated by 70 nucleotidesPf6N2E2_669 and Pf6N2E2_670 are separated by 137 nucleotidesPf6N2E2_670 and Pf6N2E2_671 are separated by 214 nucleotides Pf6N2E2_668 - 2,3-butanediol dehydrogenase, R-alcohol forming, (R)- and (S)-acetoin-specific (EC 1.1.1.4), at 773,226 to 774,320 _668 Pf6N2E2_669 - Nuclease inhibitor, at 774,391 to 774,945 _669 Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000 _670 Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243 _671 Position (kb) 775 776 777Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 774.147 kb on + strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.148 kb on - strand, within Pf6N2E2_668at 774.466 kb on - strand, within Pf6N2E2_669at 774.466 kb on - strand, within Pf6N2E2_669at 774.746 kb on - strand, within Pf6N2E2_669at 774.782 kb on + strand, within Pf6N2E2_669at 774.783 kb on - strand, within Pf6N2E2_669at 774.783 kb on - strand, within Pf6N2E2_669at 774.858 kb on - strand, within Pf6N2E2_669at 774.938 kb on + strandat 774.938 kb on + strandat 774.939 kb on - strandat 775.072 kb on + strandat 775.084 kb on + strandat 775.147 kb on + strandat 775.147 kb on + strandat 775.148 kb on - strandat 775.148 kb on - strandat 775.288 kb on + strand, within Pf6N2E2_670at 775.288 kb on + strandat 775.289 kb on - strand, within Pf6N2E2_670at 775.289 kb on - strand, within Pf6N2E2_670at 775.289 kb on - strand, within Pf6N2E2_670at 775.416 kb on + strand, within Pf6N2E2_670at 775.416 kb on + strand, within Pf6N2E2_670at 775.416 kb on + strand, within Pf6N2E2_670at 775.417 kb on - strand, within Pf6N2E2_670at 775.570 kb on + strand, within Pf6N2E2_670at 775.570 kb on + strand, within Pf6N2E2_670at 775.571 kb on - strand, within Pf6N2E2_670at 775.571 kb on - strand, within Pf6N2E2_670at 775.588 kb on + strand, within Pf6N2E2_670at 775.589 kb on - strand, within Pf6N2E2_670at 775.589 kb on - strand, within Pf6N2E2_670at 775.589 kb on - strand, within Pf6N2E2_670at 775.663 kb on + strand, within Pf6N2E2_670at 775.663 kb on + strand, within Pf6N2E2_670at 776.032 kb on + strandat 776.034 kb on + strandat 776.034 kb on + strandat 776.034 kb on + strandat 776.034 kb on + strandat 776.034 kb on + strandat 776.034 kb on + strandat 776.034 kb on + strandat 776.035 kb on - strandat 776.035 kb on - strandat 776.035 kb on - strandat 776.035 kb on - strandat 776.042 kb on + strandat 776.043 kb on - strandat 776.057 kb on + strandat 776.057 kb on + strandat 776.057 kb on + strandat 776.057 kb on + strandat 776.057 kb on + strandat 776.058 kb on - strandat 776.058 kb on - strandat 776.061 kb on + strandat 776.062 kb on - strandat 776.062 kb on - strandat 776.062 kb on - strandat 776.117 kb on + strandat 776.117 kb on + strandat 776.141 kb on + strandat 776.328 kb on - strand, within Pf6N2E2_671at 776.406 kb on + strand, within Pf6N2E2_671at 776.406 kb on + strand, within Pf6N2E2_671at 776.454 kb on + strand, within Pf6N2E2_671at 776.454 kb on + strand, within Pf6N2E2_671at 776.739 kb on - strand, within Pf6N2E2_671

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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774,147 + Pf6N2E2_668 0.84 -0.7
774,148 - Pf6N2E2_668 0.84 +0.3
774,148 - Pf6N2E2_668 0.84 +0.3
774,148 - Pf6N2E2_668 0.84 +0.3
774,466 - Pf6N2E2_669 0.14 -0.7
774,466 - Pf6N2E2_669 0.14 +0.3
774,746 - Pf6N2E2_669 0.64 -0.5
774,782 + Pf6N2E2_669 0.70 +0.3
774,783 - Pf6N2E2_669 0.71 +0.3
774,783 - Pf6N2E2_669 0.71 -1.7
774,858 - Pf6N2E2_669 0.84 +0.3
774,938 + +0.3
774,938 + -0.7
774,939 - +0.3
775,072 + -0.4
775,084 + +0.3
775,147 + -0.8
775,147 + +0.2
775,148 - -0.8
775,148 - +0.2
775,288 + Pf6N2E2_670 0.22 +0.2
775,288 + +0.2
775,289 - Pf6N2E2_670 0.22 +0.2
775,289 - Pf6N2E2_670 0.22 +0.2
775,289 - Pf6N2E2_670 0.22 +0.6
775,416 + Pf6N2E2_670 0.36 -0.9
775,416 + Pf6N2E2_670 0.36 +0.2
775,416 + Pf6N2E2_670 0.36 -2.1
775,417 - Pf6N2E2_670 0.36 +0.2
775,570 + Pf6N2E2_670 0.53 -3.1
775,570 + Pf6N2E2_670 0.53 -1.2
775,571 - Pf6N2E2_670 0.53 -0.8
775,571 - Pf6N2E2_670 0.53 -1.3
775,588 + Pf6N2E2_670 0.55 -0.5
775,589 - Pf6N2E2_670 0.55 -0.8
775,589 - Pf6N2E2_670 0.55 +0.2
775,589 - Pf6N2E2_670 0.55 +0.2
775,663 + Pf6N2E2_670 0.63 -0.3
775,663 + Pf6N2E2_670 0.63 -1.8
776,032 + +0.2
776,034 + +0.2
776,034 + -0.8
776,034 + +0.2
776,034 + +1.2
776,034 + +0.2
776,034 + -0.2
776,034 + +0.2
776,035 - -0.4
776,035 - +0.2
776,035 - -0.8
776,035 - -0.8
776,042 + +0.2
776,043 - -0.8
776,057 + -0.6
776,057 + +0.2
776,057 + -0.2
776,057 + -0.8
776,057 + -0.8
776,058 - +0.2
776,058 - -6.2
776,061 + +0.2
776,062 - +0.2
776,062 - +0.7
776,062 - -1.3
776,117 + -0.8
776,117 + -0.8
776,141 + +0.3
776,328 - Pf6N2E2_671 0.11 -1.3
776,406 + Pf6N2E2_671 0.19 -1.7
776,406 + Pf6N2E2_671 0.19 +0.3
776,454 + Pf6N2E2_671 0.23 -0.7
776,454 + Pf6N2E2_671 0.23 -0.7
776,739 - Pf6N2E2_671 0.51 +0.3

Or see this region's nucleotide sequence