Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_614 and Pf6N2E2_615 are separated by 156 nucleotides Pf6N2E2_615 and Pf6N2E2_616 are separated by 173 nucleotides Pf6N2E2_616 and Pf6N2E2_617 are separated by 143 nucleotides Pf6N2E2_617 and Pf6N2E2_618 overlap by 4 nucleotides
Pf6N2E2_614 - 3-carboxymuconate cyclase, at 697,856 to 699,031
_614
Pf6N2E2_615 - FIG00957600: hypothetical protein, at 699,188 to 699,367
_615
Pf6N2E2_616 - hypothetical protein, at 699,541 to 699,666
_616
Pf6N2E2_617 - Probable glutathione S-transferase, at 699,810 to 700,412
_617
Pf6N2E2_618 - Exonuclease SbcC, at 700,409 to 704,050
_618
Position (kb) 699
700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 699.067 kb on + strand at 699.068 kb on - strand at 699.068 kb on - strand at 699.098 kb on - strand at 699.299 kb on + strand, within Pf6N2E2_615 at 699.299 kb on + strand, within Pf6N2E2_615 at 699.300 kb on - strand, within Pf6N2E2_615 at 699.311 kb on + strand, within Pf6N2E2_615 at 699.311 kb on + strand, within Pf6N2E2_615 at 699.312 kb on - strand, within Pf6N2E2_615 at 699.335 kb on + strand, within Pf6N2E2_615 at 699.527 kb on + strand at 699.527 kb on + strand at 699.528 kb on - strand at 699.578 kb on - strand, within Pf6N2E2_616 at 699.812 kb on - strand at 700.076 kb on - strand, within Pf6N2E2_617 at 700.076 kb on - strand, within Pf6N2E2_617 at 700.081 kb on + strand, within Pf6N2E2_617 at 700.081 kb on + strand, within Pf6N2E2_617 at 700.082 kb on - strand, within Pf6N2E2_617 at 700.394 kb on - strand at 700.394 kb on - strand at 700.394 kb on - strand at 700.559 kb on + strand at 700.697 kb on + strand at 700.697 kb on + strand at 700.697 kb on + strand at 700.698 kb on - strand at 700.771 kb on + strand at 700.771 kb on + strand at 700.771 kb on + strand at 700.772 kb on - strand at 700.772 kb on - strand at 700.782 kb on - strand, within Pf6N2E2_618 at 700.833 kb on + strand, within Pf6N2E2_618 at 700.908 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.917 kb on + strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.918 kb on - strand, within Pf6N2E2_618 at 700.991 kb on + strand, within Pf6N2E2_618 at 700.991 kb on + strand, within Pf6N2E2_618 at 701.345 kb on + strand, within Pf6N2E2_618 at 701.346 kb on - strand, within Pf6N2E2_618 at 701.346 kb on - strand, within Pf6N2E2_618
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 699,067 + -0.7 699,068 - +0.3 699,068 - -0.7 699,098 - +0.3 699,299 + Pf6N2E2_615 0.62 +0.3 699,299 + Pf6N2E2_615 0.62 +0.6 699,300 - Pf6N2E2_615 0.62 +0.3 699,311 + Pf6N2E2_615 0.68 +0.3 699,311 + Pf6N2E2_615 0.68 -1.8 699,312 - Pf6N2E2_615 0.69 +0.3 699,335 + Pf6N2E2_615 0.82 +0.3 699,527 + -3.1 699,527 + -1.3 699,528 - +0.3 699,578 - Pf6N2E2_616 0.29 -0.7 699,812 - -1.4 700,076 - Pf6N2E2_617 0.44 +0.3 700,076 - Pf6N2E2_617 0.44 -1.5 700,081 + Pf6N2E2_617 0.45 -1.3 700,081 + Pf6N2E2_617 0.45 +0.3 700,082 - Pf6N2E2_617 0.45 -0.7 700,394 - -0.5 700,394 - -0.7 700,394 - -2.7 700,559 + +0.3 700,697 + +0.3 700,697 + +0.3 700,697 + -0.7 700,698 - +0.3 700,771 + +0.3 700,771 + -1.3 700,771 + +0.3 700,772 - -1.1 700,772 - -0.7 700,782 - Pf6N2E2_618 0.10 -0.5 700,833 + Pf6N2E2_618 0.12 +0.3 700,908 + Pf6N2E2_618 0.14 +0.3 700,917 + Pf6N2E2_618 0.14 -1.0 700,917 + Pf6N2E2_618 0.14 +0.3 700,917 + Pf6N2E2_618 0.14 -0.7 700,917 + Pf6N2E2_618 0.14 -2.1 700,917 + Pf6N2E2_618 0.14 -1.1 700,918 - Pf6N2E2_618 0.14 +0.3 700,918 - Pf6N2E2_618 0.14 -1.5 700,918 - Pf6N2E2_618 0.14 +0.3 700,991 + Pf6N2E2_618 0.16 +0.3 700,991 + Pf6N2E2_618 0.16 -0.7 701,345 + Pf6N2E2_618 0.26 +0.3 701,346 - Pf6N2E2_618 0.26 +0.3 701,346 - Pf6N2E2_618 0.26 -0.9
Or see this region's nucleotide sequence