Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6064 and Pf6N2E2_6065 are separated by 336 nucleotides Pf6N2E2_6065 and Pf6N2E2_6066 are separated by 0 nucleotides Pf6N2E2_6066 and Pf6N2E2_6067 overlap by 1 nucleotides
Pf6N2E2_6064 - 2-methylaconitate isomerase, at 6,885,726 to 6,886,919
_6064
Pf6N2E2_6065 - ABC transporter in pyoverdin gene cluster, periplasmic component, at 6,887,256 to 6,888,149
_6065
Pf6N2E2_6066 - ABC transporter in pyoverdin gene cluster, permease component, at 6,888,150 to 6,889,049
_6066
Pf6N2E2_6067 - ABC transporter in pyoverdin gene cluster, ATP-binding component, at 6,889,049 to 6,889,801
_6067
Position (kb)
6887
6888
6889 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6886.587 kb on + strand, within Pf6N2E2_6064 at 6886.587 kb on + strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.588 kb on - strand, within Pf6N2E2_6064 at 6886.633 kb on + strand, within Pf6N2E2_6064 at 6886.633 kb on + strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6886.634 kb on - strand, within Pf6N2E2_6064 at 6887.105 kb on - strand at 6887.199 kb on + strand at 6887.947 kb on + strand, within Pf6N2E2_6065 at 6888.038 kb on - strand, within Pf6N2E2_6065 at 6888.151 kb on + strand at 6888.152 kb on - strand at 6888.152 kb on - strand at 6888.458 kb on - strand, within Pf6N2E2_6066 at 6888.458 kb on - strand, within Pf6N2E2_6066 at 6888.532 kb on + strand, within Pf6N2E2_6066 at 6888.533 kb on - strand, within Pf6N2E2_6066 at 6888.533 kb on - strand, within Pf6N2E2_6066 at 6888.533 kb on - strand, within Pf6N2E2_6066 at 6888.708 kb on + strand, within Pf6N2E2_6066 at 6888.709 kb on - strand, within Pf6N2E2_6066 at 6888.709 kb on - strand, within Pf6N2E2_6066 at 6888.709 kb on - strand, within Pf6N2E2_6066 at 6888.755 kb on - strand, within Pf6N2E2_6066 at 6888.815 kb on - strand, within Pf6N2E2_6066 at 6888.904 kb on - strand, within Pf6N2E2_6066 at 6888.904 kb on - strand, within Pf6N2E2_6066 at 6889.042 kb on + strand at 6889.042 kb on + strand at 6889.042 kb on + strand at 6889.042 kb on + strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.043 kb on - strand at 6889.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 6,886,587 + Pf6N2E2_6064 0.72 -2.0 6,886,587 + Pf6N2E2_6064 0.72 +0.4 6,886,588 - Pf6N2E2_6064 0.72 -0.6 6,886,588 - Pf6N2E2_6064 0.72 +0.4 6,886,588 - Pf6N2E2_6064 0.72 -3.8 6,886,588 - Pf6N2E2_6064 0.72 +0.4 6,886,633 + Pf6N2E2_6064 0.76 +0.4 6,886,633 + Pf6N2E2_6064 0.76 -0.6 6,886,634 - Pf6N2E2_6064 0.76 -1.6 6,886,634 - Pf6N2E2_6064 0.76 +0.4 6,886,634 - Pf6N2E2_6064 0.76 -1.2 6,886,634 - Pf6N2E2_6064 0.76 +0.4 6,887,105 - +0.4 6,887,199 + +0.4 6,887,947 + Pf6N2E2_6065 0.77 -1.2 6,888,038 - Pf6N2E2_6065 0.87 -0.6 6,888,151 + -2.5 6,888,152 - -0.1 6,888,152 - -0.4 6,888,458 - Pf6N2E2_6066 0.34 -0.6 6,888,458 - Pf6N2E2_6066 0.34 +0.4 6,888,532 + Pf6N2E2_6066 0.42 -0.6 6,888,533 - Pf6N2E2_6066 0.43 +0.4 6,888,533 - Pf6N2E2_6066 0.43 -0.6 6,888,533 - Pf6N2E2_6066 0.43 +0.4 6,888,708 + Pf6N2E2_6066 0.62 +0.4 6,888,709 - Pf6N2E2_6066 0.62 +0.4 6,888,709 - Pf6N2E2_6066 0.62 -0.2 6,888,709 - Pf6N2E2_6066 0.62 -1.6 6,888,755 - Pf6N2E2_6066 0.67 -0.6 6,888,815 - Pf6N2E2_6066 0.74 +0.4 6,888,904 - Pf6N2E2_6066 0.84 -0.5 6,888,904 - Pf6N2E2_6066 0.84 -0.6 6,889,042 + +0.4 6,889,042 + +0.4 6,889,042 + -0.6 6,889,042 + -0.6 6,889,043 - -0.6 6,889,043 - -0.6 6,889,043 - +0.4 6,889,043 - +0.4 6,889,043 - -0.8
Or see this region's nucleotide sequence