Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6060 and Pf6N2E2_6061 overlap by 4 nucleotides Pf6N2E2_6061 and Pf6N2E2_6062 are separated by 41 nucleotides Pf6N2E2_6062 and Pf6N2E2_6063 are separated by 177 nucleotides
Pf6N2E2_6060 - Propionate catabolism operon transcriptional regulator of GntR family [predicted], at 6,880,154 to 6,880,864
_6060
Pf6N2E2_6061 - Methylisocitrate lyase (EC 4.1.3.30) (from data) , at 6,880,861 to 6,881,745
_6061
Pf6N2E2_6062 - 2-methylcitrate synthase (EC 2.3.3.5) (from data) , at 6,881,787 to 6,882,914
_6062
Pf6N2E2_6063 - 2-methylcitrate dehydratase FeS dependent (EC 4.2.1.79), at 6,883,092 to 6,885,683
_6063
Position (kb)
6881
6882
6883 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6880.987 kb on + strand, within Pf6N2E2_6061 at 6880.987 kb on + strand, within Pf6N2E2_6061 at 6880.988 kb on - strand, within Pf6N2E2_6061 at 6880.988 kb on - strand, within Pf6N2E2_6061 at 6881.036 kb on + strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.037 kb on - strand, within Pf6N2E2_6061 at 6881.200 kb on - strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.446 kb on + strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.447 kb on - strand, within Pf6N2E2_6061 at 6881.519 kb on - strand, within Pf6N2E2_6061 at 6881.611 kb on + strand, within Pf6N2E2_6061 at 6881.612 kb on - strand, within Pf6N2E2_6061 at 6881.612 kb on - strand, within Pf6N2E2_6061 at 6881.695 kb on + strand at 6881.695 kb on + strand at 6881.743 kb on + strand at 6881.743 kb on + strand at 6881.744 kb on - strand at 6881.744 kb on - strand at 6881.744 kb on - strand at 6881.803 kb on + strand at 6881.803 kb on + strand at 6881.886 kb on + strand at 6881.991 kb on + strand, within Pf6N2E2_6062 at 6881.992 kb on - strand, within Pf6N2E2_6062 at 6882.193 kb on - strand, within Pf6N2E2_6062 at 6882.252 kb on - strand, within Pf6N2E2_6062 at 6882.389 kb on + strand, within Pf6N2E2_6062 at 6882.389 kb on + strand, within Pf6N2E2_6062 at 6882.390 kb on - strand, within Pf6N2E2_6062 at 6882.390 kb on - strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.576 kb on + strand, within Pf6N2E2_6062 at 6882.577 kb on - strand, within Pf6N2E2_6062 at 6882.578 kb on + strand, within Pf6N2E2_6062 at 6882.726 kb on + strand, within Pf6N2E2_6062 at 6882.727 kb on - strand, within Pf6N2E2_6062 at 6882.867 kb on + strand at 6882.868 kb on - strand at 6882.935 kb on - strand at 6882.935 kb on - strand at 6882.935 kb on - strand at 6883.137 kb on + strand at 6883.182 kb on + strand at 6883.183 kb on - strand at 6883.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 6,880,987 + Pf6N2E2_6061 0.14 +0.4 6,880,987 + Pf6N2E2_6061 0.14 -1.6 6,880,988 - Pf6N2E2_6061 0.14 -2.1 6,880,988 - Pf6N2E2_6061 0.14 +0.4 6,881,036 + Pf6N2E2_6061 0.20 -2.0 6,881,037 - Pf6N2E2_6061 0.20 -2.2 6,881,037 - Pf6N2E2_6061 0.20 +0.4 6,881,037 - Pf6N2E2_6061 0.20 -1.5 6,881,200 - Pf6N2E2_6061 0.38 +0.4 6,881,446 + Pf6N2E2_6061 0.66 +0.4 6,881,446 + Pf6N2E2_6061 0.66 -1.6 6,881,446 + Pf6N2E2_6061 0.66 +0.4 6,881,446 + Pf6N2E2_6061 0.66 -2.1 6,881,446 + Pf6N2E2_6061 0.66 +0.4 6,881,446 + Pf6N2E2_6061 0.66 +0.4 6,881,447 - Pf6N2E2_6061 0.66 -2.1 6,881,447 - Pf6N2E2_6061 0.66 -1.6 6,881,447 - Pf6N2E2_6061 0.66 -2.0 6,881,447 - Pf6N2E2_6061 0.66 -0.6 6,881,447 - Pf6N2E2_6061 0.66 -1.6 6,881,447 - Pf6N2E2_6061 0.66 +0.4 6,881,447 - Pf6N2E2_6061 0.66 +1.4 6,881,519 - Pf6N2E2_6061 0.74 -2.2 6,881,611 + Pf6N2E2_6061 0.85 +0.4 6,881,612 - Pf6N2E2_6061 0.85 -2.5 6,881,612 - Pf6N2E2_6061 0.85 +0.4 6,881,695 + -0.6 6,881,695 + +0.4 6,881,743 + +0.4 6,881,743 + +0.4 6,881,744 - +0.4 6,881,744 - -0.6 6,881,744 - +0.4 6,881,803 + +0.4 6,881,803 + +0.4 6,881,886 + -0.6 6,881,991 + Pf6N2E2_6062 0.18 +0.4 6,881,992 - Pf6N2E2_6062 0.18 -1.6 6,882,193 - Pf6N2E2_6062 0.36 +0.4 6,882,252 - Pf6N2E2_6062 0.41 +0.4 6,882,389 + Pf6N2E2_6062 0.53 -1.8 6,882,389 + Pf6N2E2_6062 0.53 -0.6 6,882,390 - Pf6N2E2_6062 0.53 -0.6 6,882,390 - Pf6N2E2_6062 0.53 -0.6 6,882,576 + Pf6N2E2_6062 0.70 -0.6 6,882,576 + Pf6N2E2_6062 0.70 -2.8 6,882,576 + Pf6N2E2_6062 0.70 +0.4 6,882,576 + Pf6N2E2_6062 0.70 +0.4 6,882,576 + Pf6N2E2_6062 0.70 -3.4 6,882,577 - Pf6N2E2_6062 0.70 -1.2 6,882,578 + Pf6N2E2_6062 0.70 +0.4 6,882,726 + Pf6N2E2_6062 0.83 +0.4 6,882,727 - Pf6N2E2_6062 0.83 +0.4 6,882,867 + +0.4 6,882,868 - -2.5 6,882,935 - +0.4 6,882,935 - -0.6 6,882,935 - +0.4 6,883,137 + +0.4 6,883,182 + -3.6 6,883,183 - +0.4 6,883,197 + +0.4
Or see this region's nucleotide sequence