Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5260 and Pf6N2E2_5261 are separated by 55 nucleotides Pf6N2E2_5261 and Pf6N2E2_5262 are separated by 142 nucleotides Pf6N2E2_5262 and Pf6N2E2_5263 are separated by 121 nucleotides Pf6N2E2_5263 and Pf6N2E2_5264 are separated by 215 nucleotides Pf6N2E2_5264 and Pf6N2E2_5265 overlap by 33 nucleotides
Pf6N2E2_5260 - Phosphate acetyltransferase (EC 2.3.1.8), at 6,055,033 to 6,057,132
_5260
Pf6N2E2_5261 - FIG00956983: hypothetical protein, at 6,057,188 to 6,057,358
_5261
Pf6N2E2_5262 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 6,057,501 to 6,057,986
_5262
Pf6N2E2_5263 - Glutathione peroxidase family protein, at 6,058,108 to 6,058,590
_5263
Pf6N2E2_5264 - hypothetical protein, at 6,058,806 to 6,058,940
_5264
Pf6N2E2_5265 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 6,058,908 to 6,060,011
_5265
Position (kb)
6057
6058 Strain fitness (log2 ratio)
-2
-1
0
1 at 6056.786 kb on + strand, within Pf6N2E2_5260 at 6056.786 kb on + strand, within Pf6N2E2_5260 at 6056.787 kb on - strand, within Pf6N2E2_5260 at 6056.787 kb on - strand, within Pf6N2E2_5260 at 6056.856 kb on - strand, within Pf6N2E2_5260 at 6057.060 kb on + strand at 6057.117 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.169 kb on + strand at 6057.170 kb on - strand at 6057.170 kb on - strand at 6057.170 kb on - strand at 6057.170 kb on - strand at 6057.170 kb on - strand at 6057.170 kb on - strand at 6057.170 kb on - strand at 6057.183 kb on + strand at 6057.183 kb on + strand at 6057.183 kb on + strand at 6057.184 kb on - strand at 6057.184 kb on - strand at 6057.184 kb on - strand at 6057.257 kb on + strand, within Pf6N2E2_5261 at 6057.458 kb on + strand at 6057.458 kb on + strand at 6057.458 kb on + strand at 6057.459 kb on - strand at 6057.481 kb on + strand at 6057.482 kb on - strand at 6057.482 kb on - strand at 6057.518 kb on + strand at 6057.518 kb on + strand at 6057.519 kb on - strand at 6057.537 kb on + strand at 6057.537 kb on + strand at 6057.538 kb on - strand at 6057.538 kb on - strand at 6057.539 kb on + strand at 6057.540 kb on - strand at 6057.601 kb on - strand, within Pf6N2E2_5262 at 6057.702 kb on + strand, within Pf6N2E2_5262 at 6057.702 kb on + strand, within Pf6N2E2_5262 at 6057.703 kb on - strand, within Pf6N2E2_5262 at 6057.997 kb on - strand at 6058.044 kb on + strand at 6058.058 kb on + strand at 6058.058 kb on + strand at 6058.059 kb on - strand at 6058.059 kb on - strand at 6058.128 kb on + strand at 6058.128 kb on + strand at 6058.129 kb on - strand at 6058.232 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.253 kb on - strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.261 kb on + strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.262 kb on - strand, within Pf6N2E2_5263 at 6058.470 kb on + strand, within Pf6N2E2_5263 at 6058.471 kb on - strand, within Pf6N2E2_5263 at 6058.471 kb on - strand, within Pf6N2E2_5263 at 6058.672 kb on + strand at 6058.690 kb on + strand at 6058.807 kb on + strand at 6058.808 kb on - strand at 6058.808 kb on - strand at 6058.808 kb on - strand at 6058.815 kb on - strand at 6058.830 kb on + strand, within Pf6N2E2_5264 at 6058.837 kb on - strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.859 kb on + strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.860 kb on - strand, within Pf6N2E2_5264 at 6058.863 kb on - strand, within Pf6N2E2_5264 at 6058.915 kb on + strand at 6058.916 kb on - strand at 6058.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 6,056,786 + Pf6N2E2_5260 0.83 -1.6 6,056,786 + Pf6N2E2_5260 0.83 +0.4 6,056,787 - Pf6N2E2_5260 0.84 -0.9 6,056,787 - Pf6N2E2_5260 0.84 -0.8 6,056,856 - Pf6N2E2_5260 0.87 -0.6 6,057,060 + -0.1 6,057,117 + -0.2 6,057,169 + -0.6 6,057,169 + +0.4 6,057,169 + +0.4 6,057,169 + +0.4 6,057,169 + +0.4 6,057,169 + +0.4 6,057,169 + -0.6 6,057,169 + -0.6 6,057,170 - -0.3 6,057,170 - -0.2 6,057,170 - -0.0 6,057,170 - -0.7 6,057,170 - +0.4 6,057,170 - -0.6 6,057,170 - -0.6 6,057,183 + +0.4 6,057,183 + +0.4 6,057,183 + -0.6 6,057,184 - +0.4 6,057,184 - -0.6 6,057,184 - -2.4 6,057,257 + Pf6N2E2_5261 0.40 -0.5 6,057,458 + +0.4 6,057,458 + -0.6 6,057,458 + -0.7 6,057,459 - +0.4 6,057,481 + +0.6 6,057,482 - -0.6 6,057,482 - -0.3 6,057,518 + -1.2 6,057,518 + -0.5 6,057,519 - -1.6 6,057,537 + +0.4 6,057,537 + -0.6 6,057,538 - +0.4 6,057,538 - -0.6 6,057,539 + -0.2 6,057,540 - -1.0 6,057,601 - Pf6N2E2_5262 0.21 +0.4 6,057,702 + Pf6N2E2_5262 0.41 -2.6 6,057,702 + Pf6N2E2_5262 0.41 -0.6 6,057,703 - Pf6N2E2_5262 0.42 -0.6 6,057,997 - +0.4 6,058,044 + -0.6 6,058,058 + +0.2 6,058,058 + -0.6 6,058,059 - +0.4 6,058,059 - -0.7 6,058,128 + -0.3 6,058,128 + +0.4 6,058,129 - +0.4 6,058,232 - Pf6N2E2_5263 0.26 -1.2 6,058,253 - Pf6N2E2_5263 0.30 -0.6 6,058,253 - Pf6N2E2_5263 0.30 +0.4 6,058,253 - Pf6N2E2_5263 0.30 -0.0 6,058,261 + Pf6N2E2_5263 0.32 -1.2 6,058,261 + Pf6N2E2_5263 0.32 -0.6 6,058,261 + Pf6N2E2_5263 0.32 +0.4 6,058,262 - Pf6N2E2_5263 0.32 -1.2 6,058,262 - Pf6N2E2_5263 0.32 -0.6 6,058,262 - Pf6N2E2_5263 0.32 -0.6 6,058,262 - Pf6N2E2_5263 0.32 +0.4 6,058,262 - Pf6N2E2_5263 0.32 +0.4 6,058,262 - Pf6N2E2_5263 0.32 +0.4 6,058,262 - Pf6N2E2_5263 0.32 -1.2 6,058,262 - Pf6N2E2_5263 0.32 +0.4 6,058,470 + Pf6N2E2_5263 0.75 -0.3 6,058,471 - Pf6N2E2_5263 0.75 +0.4 6,058,471 - Pf6N2E2_5263 0.75 -0.6 6,058,672 + -1.2 6,058,690 + +0.4 6,058,807 + -0.8 6,058,808 - +0.4 6,058,808 - -0.6 6,058,808 - +0.7 6,058,815 - -0.6 6,058,830 + Pf6N2E2_5264 0.18 +0.4 6,058,837 - Pf6N2E2_5264 0.23 -1.2 6,058,859 + Pf6N2E2_5264 0.39 -0.6 6,058,859 + Pf6N2E2_5264 0.39 -0.6 6,058,859 + Pf6N2E2_5264 0.39 +0.4 6,058,859 + Pf6N2E2_5264 0.39 -1.2 6,058,860 - Pf6N2E2_5264 0.40 -1.1 6,058,860 - Pf6N2E2_5264 0.40 +0.4 6,058,860 - Pf6N2E2_5264 0.40 -1.2 6,058,863 - Pf6N2E2_5264 0.42 -1.2 6,058,915 + -0.8 6,058,916 - -1.2 6,058,916 - +0.4
Or see this region's nucleotide sequence