Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_497

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_496 and Pf6N2E2_497 are separated by 0 nucleotidesPf6N2E2_497 and Pf6N2E2_498 are separated by 183 nucleotides Pf6N2E2_496 - Dihydropyrimidine dehydrogenase (NADP(+)) (EC 1.3.1.2) (from data), at 562,747 to 564,021 _496 Pf6N2E2_497 - Pyridine nucleotide-disulphide oxidoreductase associated with reductive pyrimidine catabolism, at 564,022 to 565,389 _497 Pf6N2E2_498 - Dihydropyrimidinase (EC 3.5.2.2) (from data), at 565,573 to 567,012 _498 Position (kb) 564 565 566Strain fitness (log2 ratio) -2 -1 0 1 2at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.022 kb on - strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.036 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.522 kb on + strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.523 kb on - strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.555 kb on + strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496at 563.556 kb on - strand, within Pf6N2E2_496at 563.843 kb on + strand, within Pf6N2E2_496at 563.936 kb on + strandat 563.937 kb on - strandat 563.937 kb on - strandat 563.937 kb on - strandat 563.937 kb on - strandat 563.937 kb on - strandat 564.024 kb on - strandat 564.141 kb on - strandat 564.141 kb on - strandat 564.617 kb on + strand, within Pf6N2E2_497at 564.617 kb on + strand, within Pf6N2E2_497at 564.815 kb on + strand, within Pf6N2E2_497at 564.815 kb on + strand, within Pf6N2E2_497at 564.833 kb on + strand, within Pf6N2E2_497at 564.834 kb on - strand, within Pf6N2E2_497at 564.834 kb on - strand, within Pf6N2E2_497at 564.834 kb on - strand, within Pf6N2E2_497at 565.028 kb on + strand, within Pf6N2E2_497at 565.197 kb on + strand, within Pf6N2E2_497at 565.198 kb on - strand, within Pf6N2E2_497at 565.256 kb on + strandat 565.262 kb on + strandat 565.262 kb on + strandat 565.656 kb on + strandat 565.658 kb on + strandat 565.658 kb on + strandat 565.658 kb on + strandat 565.658 kb on + strandat 565.684 kb on + strandat 565.684 kb on + strandat 566.224 kb on + strand, within Pf6N2E2_498at 566.224 kb on + strand, within Pf6N2E2_498at 566.234 kb on + strand, within Pf6N2E2_498at 566.262 kb on - strand, within Pf6N2E2_498at 566.306 kb on + strand, within Pf6N2E2_498

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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563,022 - Pf6N2E2_496 0.22 -1.1
563,022 - Pf6N2E2_496 0.22 +0.3
563,022 - Pf6N2E2_496 0.22 +0.3
563,036 + Pf6N2E2_496 0.23 +0.3
563,036 + Pf6N2E2_496 0.23 -0.2
563,036 + Pf6N2E2_496 0.23 +0.3
563,522 + Pf6N2E2_496 0.61 -0.7
563,522 + Pf6N2E2_496 0.61 +0.3
563,523 - Pf6N2E2_496 0.61 -2.3
563,523 - Pf6N2E2_496 0.61 +0.3
563,523 - Pf6N2E2_496 0.61 -2.7
563,523 - Pf6N2E2_496 0.61 +0.3
563,523 - Pf6N2E2_496 0.61 -1.1
563,523 - Pf6N2E2_496 0.61 +0.3
563,555 + Pf6N2E2_496 0.63 -1.1
563,555 + Pf6N2E2_496 0.63 +0.3
563,556 - Pf6N2E2_496 0.63 +0.3
563,556 - Pf6N2E2_496 0.63 -0.7
563,843 + Pf6N2E2_496 0.86 -1.1
563,936 + +0.3
563,937 - +0.3
563,937 - -1.7
563,937 - -0.7
563,937 - -0.5
563,937 - -1.0
564,024 - +0.3
564,141 - +1.3
564,141 - +0.3
564,617 + Pf6N2E2_497 0.43 +0.3
564,617 + Pf6N2E2_497 0.43 +0.3
564,815 + Pf6N2E2_497 0.58 +0.3
564,815 + Pf6N2E2_497 0.58 -0.7
564,833 + Pf6N2E2_497 0.59 -1.1
564,834 - Pf6N2E2_497 0.59 -2.4
564,834 - Pf6N2E2_497 0.59 +0.3
564,834 - Pf6N2E2_497 0.59 -1.3
565,028 + Pf6N2E2_497 0.74 -1.3
565,197 + Pf6N2E2_497 0.86 -0.7
565,198 - Pf6N2E2_497 0.86 -0.7
565,256 + +0.3
565,262 + +0.3
565,262 + +1.9
565,656 + -0.7
565,658 + +0.3
565,658 + -0.5
565,658 + -0.7
565,658 + +0.3
565,684 + -0.3
565,684 + -0.7
566,224 + Pf6N2E2_498 0.45 -0.9
566,224 + Pf6N2E2_498 0.45 +0.3
566,234 + Pf6N2E2_498 0.46 +0.3
566,262 - Pf6N2E2_498 0.48 -0.9
566,306 + Pf6N2E2_498 0.51 -0.7

Or see this region's nucleotide sequence