Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_478 and Pf6N2E2_479 are separated by 3 nucleotides Pf6N2E2_479 and Pf6N2E2_480 are separated by 0 nucleotides
Pf6N2E2_478 - Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) , at 543,500 to 544,885
_478
Pf6N2E2_479 - Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase (EC 2.3.1.168) (from data) , at 544,889 to 546,160
_479
Pf6N2E2_480 - 3-methyl-2-oxobutanoate dehydrogenase (2-methylpropanoyl-transferring) (EC 1.2.4.4) (from data) , at 546,161 to 547,219
_480
Position (kb)
544
545
546
547 Strain fitness (log2 ratio)
-2
-1
0
1 at 544.195 kb on - strand, within Pf6N2E2_478 at 544.195 kb on - strand, within Pf6N2E2_478 at 544.341 kb on + strand, within Pf6N2E2_478 at 544.342 kb on - strand, within Pf6N2E2_478 at 544.830 kb on + strand at 544.891 kb on - strand at 545.075 kb on + strand, within Pf6N2E2_479 at 545.497 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.599 kb on - strand, within Pf6N2E2_479 at 545.643 kb on + strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 545.644 kb on - strand, within Pf6N2E2_479 at 546.053 kb on + strand at 546.053 kb on + strand at 546.146 kb on - strand at 546.216 kb on + strand at 546.216 kb on + strand at 546.217 kb on - strand at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.483 kb on + strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.484 kb on - strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.498 kb on + strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand, within Pf6N2E2_480 at 546.499 kb on - strand at 546.561 kb on + strand, within Pf6N2E2_480 at 546.561 kb on + strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.562 kb on - strand, within Pf6N2E2_480 at 546.565 kb on - strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.645 kb on + strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.646 kb on - strand, within Pf6N2E2_480 at 546.721 kb on - strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.804 kb on + strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.805 kb on - strand, within Pf6N2E2_480 at 546.913 kb on - strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.918 kb on + strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 546.919 kb on - strand, within Pf6N2E2_480 at 547.032 kb on + strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.033 kb on - strand, within Pf6N2E2_480 at 547.077 kb on + strand, within Pf6N2E2_480 at 547.078 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 544,195 - Pf6N2E2_478 0.50 -0.8 544,195 - Pf6N2E2_478 0.50 +0.3 544,341 + Pf6N2E2_478 0.61 -0.7 544,342 - Pf6N2E2_478 0.61 +0.3 544,830 + +0.3 544,891 - +0.3 545,075 + Pf6N2E2_479 0.15 +0.3 545,497 - Pf6N2E2_479 0.48 -2.0 545,599 - Pf6N2E2_479 0.56 +0.3 545,599 - Pf6N2E2_479 0.56 -1.3 545,643 + Pf6N2E2_479 0.59 -2.0 545,644 - Pf6N2E2_479 0.59 -0.7 545,644 - Pf6N2E2_479 0.59 +0.3 546,053 + -0.7 546,053 + +0.3 546,146 - -2.1 546,216 + -2.2 546,216 + +0.3 546,217 - -1.7 546,483 + Pf6N2E2_480 0.30 +0.3 546,483 + Pf6N2E2_480 0.30 +0.3 546,483 + Pf6N2E2_480 0.30 +0.3 546,483 + Pf6N2E2_480 0.30 -0.7 546,483 + Pf6N2E2_480 0.30 -1.9 546,484 - Pf6N2E2_480 0.31 -0.7 546,484 - Pf6N2E2_480 0.31 +0.3 546,484 - Pf6N2E2_480 0.31 -1.0 546,484 - Pf6N2E2_480 0.31 -0.7 546,484 - Pf6N2E2_480 0.31 +0.3 546,484 - Pf6N2E2_480 0.31 +0.3 546,484 - Pf6N2E2_480 0.31 +0.3 546,484 - Pf6N2E2_480 0.31 -0.3 546,484 - Pf6N2E2_480 0.31 +0.3 546,484 - Pf6N2E2_480 0.31 +0.3 546,484 - Pf6N2E2_480 0.31 -2.7 546,498 + Pf6N2E2_480 0.32 +0.3 546,498 + Pf6N2E2_480 0.32 +0.3 546,498 + Pf6N2E2_480 0.32 -1.7 546,499 - Pf6N2E2_480 0.32 -0.7 546,499 - Pf6N2E2_480 0.32 +0.3 546,499 - -0.7 546,561 + Pf6N2E2_480 0.38 +0.3 546,561 + Pf6N2E2_480 0.38 +0.3 546,562 - Pf6N2E2_480 0.38 -0.7 546,562 - Pf6N2E2_480 0.38 -2.3 546,562 - Pf6N2E2_480 0.38 -1.3 546,565 - Pf6N2E2_480 0.38 -2.3 546,645 + Pf6N2E2_480 0.46 +0.3 546,645 + Pf6N2E2_480 0.46 -0.7 546,645 + Pf6N2E2_480 0.46 +0.3 546,646 - Pf6N2E2_480 0.46 +0.3 546,646 - Pf6N2E2_480 0.46 +0.3 546,721 - Pf6N2E2_480 0.53 +0.3 546,804 + Pf6N2E2_480 0.61 +0.3 546,804 + Pf6N2E2_480 0.61 +0.3 546,804 + Pf6N2E2_480 0.61 +0.3 546,804 + Pf6N2E2_480 0.61 -0.7 546,804 + Pf6N2E2_480 0.61 +0.3 546,805 - Pf6N2E2_480 0.61 +0.3 546,805 - Pf6N2E2_480 0.61 -0.7 546,913 - Pf6N2E2_480 0.71 +0.3 546,918 + Pf6N2E2_480 0.71 +0.3 546,918 + Pf6N2E2_480 0.71 +0.3 546,918 + Pf6N2E2_480 0.71 +0.3 546,918 + Pf6N2E2_480 0.71 +0.3 546,919 - Pf6N2E2_480 0.72 -0.7 546,919 - Pf6N2E2_480 0.72 +0.3 546,919 - Pf6N2E2_480 0.72 -0.7 546,919 - Pf6N2E2_480 0.72 -1.7 547,032 + Pf6N2E2_480 0.82 +0.3 547,033 - Pf6N2E2_480 0.82 +0.3 547,033 - Pf6N2E2_480 0.82 +0.3 547,033 - Pf6N2E2_480 0.82 -2.1 547,077 + Pf6N2E2_480 0.86 -0.7 547,078 - -0.7
Or see this region's nucleotide sequence