Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4759 and Pf6N2E2_4760 overlap by 19 nucleotides Pf6N2E2_4760 and Pf6N2E2_4761 are separated by 36 nucleotides Pf6N2E2_4761 and Pf6N2E2_4762 overlap by 8 nucleotides Pf6N2E2_4762 and Pf6N2E2_4763 overlap by 8 nucleotides
Pf6N2E2_4759 - Biotin synthase (EC 2.8.1.6), at 5,498,157 to 5,499,212
_4759
Pf6N2E2_4760 - hypothetical protein, at 5,499,194 to 5,499,337
_4760
Pf6N2E2_4761 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 5,499,374 to 5,500,552
_4761
Pf6N2E2_4762 - Biotin synthesis protein BioH, at 5,500,545 to 5,501,276
_4762
Pf6N2E2_4763 - Biotin synthesis protein BioC, at 5,501,269 to 5,502,081
_4763
Position (kb) 5499
5500
5501 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.508 kb on + strand, within Pf6N2E2_4759 at 5498.509 kb on - strand, within Pf6N2E2_4759 at 5498.509 kb on - strand, within Pf6N2E2_4759 at 5498.509 kb on - strand, within Pf6N2E2_4759 at 5498.619 kb on + strand, within Pf6N2E2_4759 at 5498.619 kb on + strand, within Pf6N2E2_4759 at 5498.620 kb on - strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.622 kb on + strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.623 kb on - strand, within Pf6N2E2_4759 at 5498.630 kb on + strand, within Pf6N2E2_4759 at 5498.630 kb on + strand, within Pf6N2E2_4759 at 5498.631 kb on - strand, within Pf6N2E2_4759 at 5498.631 kb on - strand, within Pf6N2E2_4759 at 5498.631 kb on - strand, within Pf6N2E2_4759 at 5498.656 kb on - strand, within Pf6N2E2_4759 at 5498.656 kb on - strand, within Pf6N2E2_4759 at 5498.683 kb on - strand, within Pf6N2E2_4759 at 5498.683 kb on - strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.709 kb on + strand, within Pf6N2E2_4759 at 5498.710 kb on - strand, within Pf6N2E2_4759 at 5498.710 kb on - strand, within Pf6N2E2_4759 at 5498.710 kb on - strand, within Pf6N2E2_4759 at 5499.195 kb on + strand at 5499.196 kb on - strand at 5499.196 kb on - strand at 5499.356 kb on + strand at 5499.426 kb on - strand at 5499.513 kb on - strand, within Pf6N2E2_4761 at 5499.513 kb on - strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.692 kb on + strand, within Pf6N2E2_4761 at 5499.693 kb on - strand, within Pf6N2E2_4761 at 5499.816 kb on - strand, within Pf6N2E2_4761 at 5499.872 kb on - strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.157 kb on + strand, within Pf6N2E2_4761 at 5500.158 kb on - strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.163 kb on + strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.164 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.319 kb on - strand, within Pf6N2E2_4761 at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.491 kb on + strand at 5500.492 kb on - strand at 5500.492 kb on - strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.561 kb on + strand at 5500.562 kb on - strand at 5500.562 kb on - strand at 5500.987 kb on + strand, within Pf6N2E2_4762 at 5500.987 kb on + strand, within Pf6N2E2_4762 at 5500.988 kb on - strand, within Pf6N2E2_4762 at 5501.057 kb on + strand, within Pf6N2E2_4762 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.356 kb on + strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.357 kb on - strand, within Pf6N2E2_4763 at 5501.464 kb on + strand, within Pf6N2E2_4763 at 5501.465 kb on - strand, within Pf6N2E2_4763 at 5501.465 kb on - strand, within Pf6N2E2_4763
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.5 5,498,508 + Pf6N2E2_4759 0.33 -1.1 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.9 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.5 5,498,508 + Pf6N2E2_4759 0.33 -0.5 5,498,508 + Pf6N2E2_4759 0.33 -1.1 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,508 + Pf6N2E2_4759 0.33 +0.5 5,498,509 - Pf6N2E2_4759 0.33 -0.5 5,498,509 - Pf6N2E2_4759 0.33 +0.5 5,498,509 - Pf6N2E2_4759 0.33 +0.5 5,498,619 + Pf6N2E2_4759 0.44 +0.5 5,498,619 + Pf6N2E2_4759 0.44 -0.5 5,498,620 - Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -1.1 5,498,622 + Pf6N2E2_4759 0.44 -1.1 5,498,622 + Pf6N2E2_4759 0.44 -1.9 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 +0.5 5,498,622 + Pf6N2E2_4759 0.44 +0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -0.5 5,498,622 + Pf6N2E2_4759 0.44 -1.9 5,498,622 + Pf6N2E2_4759 0.44 +1.5 5,498,623 - Pf6N2E2_4759 0.44 -1.1 5,498,623 - Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -0.5 5,498,623 - Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -0.5 5,498,623 - Pf6N2E2_4759 0.44 +0.5 5,498,623 - Pf6N2E2_4759 0.44 -0.5 5,498,630 + Pf6N2E2_4759 0.45 +0.5 5,498,630 + Pf6N2E2_4759 0.45 -0.5 5,498,631 - Pf6N2E2_4759 0.45 +0.5 5,498,631 - Pf6N2E2_4759 0.45 -0.5 5,498,631 - Pf6N2E2_4759 0.45 +0.5 5,498,656 - Pf6N2E2_4759 0.47 +0.5 5,498,656 - Pf6N2E2_4759 0.47 -0.5 5,498,683 - Pf6N2E2_4759 0.50 -1.1 5,498,683 - Pf6N2E2_4759 0.50 +0.5 5,498,709 + Pf6N2E2_4759 0.52 +0.5 5,498,709 + Pf6N2E2_4759 0.52 +0.5 5,498,709 + Pf6N2E2_4759 0.52 -0.5 5,498,709 + Pf6N2E2_4759 0.52 -1.1 5,498,709 + Pf6N2E2_4759 0.52 -1.5 5,498,709 + Pf6N2E2_4759 0.52 -1.1 5,498,710 - Pf6N2E2_4759 0.52 +0.5 5,498,710 - Pf6N2E2_4759 0.52 +0.5 5,498,710 - Pf6N2E2_4759 0.52 +0.5 5,499,195 + +0.5 5,499,196 - -0.5 5,499,196 - +0.5 5,499,356 + -1.1 5,499,426 - -1.5 5,499,513 - Pf6N2E2_4761 0.12 -0.5 5,499,513 - Pf6N2E2_4761 0.12 -0.5 5,499,692 + Pf6N2E2_4761 0.27 +0.5 5,499,692 + Pf6N2E2_4761 0.27 +0.5 5,499,692 + Pf6N2E2_4761 0.27 -0.5 5,499,692 + Pf6N2E2_4761 0.27 +0.5 5,499,693 - Pf6N2E2_4761 0.27 +0.5 5,499,816 - Pf6N2E2_4761 0.37 +0.5 5,499,872 - Pf6N2E2_4761 0.42 +0.5 5,500,157 + Pf6N2E2_4761 0.66 -1.1 5,500,157 + Pf6N2E2_4761 0.66 -0.5 5,500,157 + Pf6N2E2_4761 0.66 -0.5 5,500,157 + Pf6N2E2_4761 0.66 -1.1 5,500,157 + Pf6N2E2_4761 0.66 -0.5 5,500,158 - Pf6N2E2_4761 0.66 +0.5 5,500,163 + Pf6N2E2_4761 0.67 +0.5 5,500,163 + Pf6N2E2_4761 0.67 -0.5 5,500,163 + Pf6N2E2_4761 0.67 +0.5 5,500,163 + Pf6N2E2_4761 0.67 -0.5 5,500,163 + Pf6N2E2_4761 0.67 -0.5 5,500,163 + Pf6N2E2_4761 0.67 +1.5 5,500,163 + Pf6N2E2_4761 0.67 -1.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 +0.5 5,500,164 - Pf6N2E2_4761 0.67 -1.1 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,164 - Pf6N2E2_4761 0.67 -0.5 5,500,319 - Pf6N2E2_4761 0.80 +0.5 5,500,319 - Pf6N2E2_4761 0.80 -2.9 5,500,319 - Pf6N2E2_4761 0.80 -1.1 5,500,491 + -0.5 5,500,491 + -0.5 5,500,491 + +0.5 5,500,491 + -1.1 5,500,491 + -0.5 5,500,491 + -1.1 5,500,491 + -0.5 5,500,492 - -0.5 5,500,492 - -0.5 5,500,561 + -0.5 5,500,561 + -0.5 5,500,561 + -0.7 5,500,561 + -1.5 5,500,561 + -1.5 5,500,561 + +2.0 5,500,562 - -1.5 5,500,562 - -1.1 5,500,987 + Pf6N2E2_4762 0.60 -1.5 5,500,987 + Pf6N2E2_4762 0.60 -1.5 5,500,988 - Pf6N2E2_4762 0.61 -0.5 5,501,057 + Pf6N2E2_4762 0.70 -1.1 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 -0.5 5,501,356 + Pf6N2E2_4763 0.11 -0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 -0.8 5,501,356 + Pf6N2E2_4763 0.11 -0.5 5,501,356 + Pf6N2E2_4763 0.11 -1.5 5,501,356 + Pf6N2E2_4763 0.11 -1.9 5,501,356 + Pf6N2E2_4763 0.11 -1.1 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 -1.1 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,356 + Pf6N2E2_4763 0.11 +0.5 5,501,357 - Pf6N2E2_4763 0.11 -0.5 5,501,357 - Pf6N2E2_4763 0.11 -1.9 5,501,357 - Pf6N2E2_4763 0.11 +0.5 5,501,357 - Pf6N2E2_4763 0.11 +0.5 5,501,464 + Pf6N2E2_4763 0.24 -0.5 5,501,465 - Pf6N2E2_4763 0.24 +0.5 5,501,465 - Pf6N2E2_4763 0.24 -0.5
Or see this region's nucleotide sequence