Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4686 and Pf6N2E2_4687 are separated by 144 nucleotides Pf6N2E2_4687 and Pf6N2E2_4688 are separated by 300 nucleotides Pf6N2E2_4688 and Pf6N2E2_4689 are separated by 20 nucleotides Pf6N2E2_4689 and Pf6N2E2_4690 are separated by 177 nucleotides
Pf6N2E2_4686 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 5,420,314 to 5,421,417
_4686
Pf6N2E2_4687 - FIG00953780: hypothetical protein, at 5,421,562 to 5,421,825
_4687
Pf6N2E2_4688 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 5,422,126 to 5,423,289
_4688
Pf6N2E2_4689 - L-carnitine 3-dehydrogenase, B subunit (EC 1.1.1.108) (from data) , at 5,423,310 to 5,423,786
_4689
Pf6N2E2_4690 - L-carnitine 3-dehydrogenase, A subunit (EC 1.1.1.108) (from data) , at 5,423,964 to 5,424,929
_4690
Position (kb)
5422
5423
5424 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.127 kb on + strand, within Pf6N2E2_4686 at 5421.128 kb on - strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.130 kb on + strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.131 kb on - strand, within Pf6N2E2_4686 at 5421.207 kb on - strand, within Pf6N2E2_4686 at 5421.254 kb on - strand, within Pf6N2E2_4686 at 5421.385 kb on - strand at 5421.415 kb on + strand at 5421.416 kb on - strand at 5421.479 kb on + strand at 5421.480 kb on - strand at 5421.488 kb on + strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.489 kb on - strand at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.613 kb on - strand, within Pf6N2E2_4687 at 5421.642 kb on + strand, within Pf6N2E2_4687 at 5421.643 kb on - strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.659 kb on + strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.660 kb on - strand, within Pf6N2E2_4687 at 5421.757 kb on + strand, within Pf6N2E2_4687 at 5421.818 kb on + strand at 5421.818 kb on + strand at 5421.819 kb on - strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.922 kb on + strand at 5421.923 kb on - strand at 5421.923 kb on - strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.175 kb on + strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.176 kb on - strand at 5422.283 kb on - strand, within Pf6N2E2_4688 at 5422.627 kb on + strand, within Pf6N2E2_4688 at 5422.627 kb on + strand, within Pf6N2E2_4688 at 5422.677 kb on - strand, within Pf6N2E2_4688 at 5422.964 kb on + strand, within Pf6N2E2_4688 at 5423.051 kb on + strand, within Pf6N2E2_4688 at 5423.051 kb on + strand, within Pf6N2E2_4688 at 5423.052 kb on - strand, within Pf6N2E2_4688 at 5423.052 kb on - strand, within Pf6N2E2_4688 at 5423.052 kb on - strand, within Pf6N2E2_4688 at 5423.347 kb on + strand at 5423.348 kb on - strand at 5423.454 kb on + strand, within Pf6N2E2_4689 at 5423.454 kb on + strand, within Pf6N2E2_4689 at 5423.455 kb on - strand, within Pf6N2E2_4689 at 5423.483 kb on + strand, within Pf6N2E2_4689 at 5423.513 kb on - strand, within Pf6N2E2_4689 at 5423.513 kb on - strand, within Pf6N2E2_4689 at 5423.513 kb on - strand, within Pf6N2E2_4689 at 5423.593 kb on + strand, within Pf6N2E2_4689 at 5423.593 kb on + strand, within Pf6N2E2_4689 at 5423.593 kb on + strand, within Pf6N2E2_4689 at 5423.689 kb on + strand, within Pf6N2E2_4689 at 5423.689 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.707 kb on + strand, within Pf6N2E2_4689 at 5423.734 kb on + strand, within Pf6N2E2_4689 at 5423.735 kb on - strand, within Pf6N2E2_4689 at 5423.735 kb on - strand, within Pf6N2E2_4689 at 5423.735 kb on - strand, within Pf6N2E2_4689 at 5423.767 kb on + strand at 5423.767 kb on + strand at 5423.767 kb on + strand at 5423.768 kb on - strand at 5423.768 kb on - strand at 5423.768 kb on - strand at 5423.804 kb on - strand at 5423.804 kb on - strand at 5423.804 kb on - strand at 5423.815 kb on + strand at 5423.816 kb on - strand at 5423.866 kb on - strand at 5423.878 kb on + strand at 5423.878 kb on + strand at 5423.878 kb on + strand at 5423.892 kb on + strand at 5423.893 kb on - strand at 5423.893 kb on - strand at 5423.893 kb on - strand at 5423.893 kb on - strand at 5424.066 kb on - strand, within Pf6N2E2_4690 at 5424.066 kb on - strand, within Pf6N2E2_4690 at 5424.066 kb on - strand, within Pf6N2E2_4690 at 5424.142 kb on + strand, within Pf6N2E2_4690 at 5424.142 kb on + strand, within Pf6N2E2_4690 at 5424.142 kb on + strand, within Pf6N2E2_4690 at 5424.143 kb on - strand, within Pf6N2E2_4690 at 5424.143 kb on - strand, within Pf6N2E2_4690 at 5424.220 kb on + strand, within Pf6N2E2_4690 at 5424.220 kb on + strand, within Pf6N2E2_4690 at 5424.220 kb on + strand, within Pf6N2E2_4690 at 5424.221 kb on - strand, within Pf6N2E2_4690 at 5424.221 kb on - strand, within Pf6N2E2_4690 at 5424.221 kb on - strand, within Pf6N2E2_4690
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 5,421,127 + Pf6N2E2_4686 0.74 +0.5 5,421,127 + Pf6N2E2_4686 0.74 -0.5 5,421,128 - Pf6N2E2_4686 0.74 -1.1 5,421,130 + Pf6N2E2_4686 0.74 +0.5 5,421,130 + Pf6N2E2_4686 0.74 +0.5 5,421,130 + Pf6N2E2_4686 0.74 +0.5 5,421,130 + Pf6N2E2_4686 0.74 -0.5 5,421,130 + Pf6N2E2_4686 0.74 -1.1 5,421,130 + Pf6N2E2_4686 0.74 -0.5 5,421,131 - Pf6N2E2_4686 0.74 -0.5 5,421,131 - Pf6N2E2_4686 0.74 -1.2 5,421,207 - Pf6N2E2_4686 0.81 -0.1 5,421,254 - Pf6N2E2_4686 0.85 -1.5 5,421,385 - -2.3 5,421,415 + +1.5 5,421,416 - -1.1 5,421,479 + +0.5 5,421,480 - -0.5 5,421,488 + -0.8 5,421,489 - +0.5 5,421,489 - -1.1 5,421,489 - -0.5 5,421,489 - +0.5 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 -1.1 5,421,613 - Pf6N2E2_4687 0.19 +0.5 5,421,613 - Pf6N2E2_4687 0.19 -0.5 5,421,642 + Pf6N2E2_4687 0.30 +0.5 5,421,643 - Pf6N2E2_4687 0.31 -0.5 5,421,659 + Pf6N2E2_4687 0.37 +0.5 5,421,659 + Pf6N2E2_4687 0.37 -1.5 5,421,659 + Pf6N2E2_4687 0.37 -0.5 5,421,659 + Pf6N2E2_4687 0.37 -1.1 5,421,659 + Pf6N2E2_4687 0.37 +0.5 5,421,660 - Pf6N2E2_4687 0.37 +0.5 5,421,660 - Pf6N2E2_4687 0.37 -0.4 5,421,660 - Pf6N2E2_4687 0.37 -0.5 5,421,660 - Pf6N2E2_4687 0.37 -1.0 5,421,660 - Pf6N2E2_4687 0.37 -0.8 5,421,660 - Pf6N2E2_4687 0.37 -1.1 5,421,660 - Pf6N2E2_4687 0.37 -1.1 5,421,757 + Pf6N2E2_4687 0.74 +0.5 5,421,818 + -0.5 5,421,818 + +0.5 5,421,819 - -0.5 5,421,922 + +0.5 5,421,922 + +0.5 5,421,922 + -0.8 5,421,923 - -1.7 5,421,923 - -0.3 5,422,175 + +0.5 5,422,175 + -1.8 5,422,175 + -0.6 5,422,175 + +0.5 5,422,175 + -0.5 5,422,175 + -0.5 5,422,175 + -0.5 5,422,175 + +2.8 5,422,175 + -0.5 5,422,175 + -0.5 5,422,175 + -0.5 5,422,176 - -1.5 5,422,176 - -0.5 5,422,176 - -1.3 5,422,176 - -0.5 5,422,176 - -0.5 5,422,176 - +0.5 5,422,176 - +0.5 5,422,176 - -0.5 5,422,176 - -0.5 5,422,176 - -0.3 5,422,176 - +0.5 5,422,176 - +0.3 5,422,176 - -1.7 5,422,283 - Pf6N2E2_4688 0.13 -0.5 5,422,627 + Pf6N2E2_4688 0.43 -0.5 5,422,627 + Pf6N2E2_4688 0.43 +0.5 5,422,677 - Pf6N2E2_4688 0.47 +0.5 5,422,964 + Pf6N2E2_4688 0.72 -0.2 5,423,051 + Pf6N2E2_4688 0.79 -0.2 5,423,051 + Pf6N2E2_4688 0.79 -1.8 5,423,052 - Pf6N2E2_4688 0.80 -0.5 5,423,052 - Pf6N2E2_4688 0.80 -1.5 5,423,052 - Pf6N2E2_4688 0.80 +0.1 5,423,347 + +1.5 5,423,348 - -1.5 5,423,454 + Pf6N2E2_4689 0.30 +0.5 5,423,454 + Pf6N2E2_4689 0.30 +0.5 5,423,455 - Pf6N2E2_4689 0.30 -0.5 5,423,483 + Pf6N2E2_4689 0.36 +0.5 5,423,513 - Pf6N2E2_4689 0.43 -0.6 5,423,513 - Pf6N2E2_4689 0.43 -1.0 5,423,513 - Pf6N2E2_4689 0.43 +0.5 5,423,593 + Pf6N2E2_4689 0.59 +0.5 5,423,593 + Pf6N2E2_4689 0.59 -0.4 5,423,593 + Pf6N2E2_4689 0.59 -1.2 5,423,689 + Pf6N2E2_4689 0.79 -0.5 5,423,689 + Pf6N2E2_4689 0.79 -1.3 5,423,707 + Pf6N2E2_4689 0.83 +0.5 5,423,707 + Pf6N2E2_4689 0.83 +0.5 5,423,707 + Pf6N2E2_4689 0.83 -0.5 5,423,707 + Pf6N2E2_4689 0.83 -1.3 5,423,734 + Pf6N2E2_4689 0.89 -0.6 5,423,735 - Pf6N2E2_4689 0.89 -2.3 5,423,735 - Pf6N2E2_4689 0.89 +0.5 5,423,735 - Pf6N2E2_4689 0.89 -0.5 5,423,767 + +0.5 5,423,767 + +0.5 5,423,767 + -0.5 5,423,768 - +0.5 5,423,768 - +0.5 5,423,768 - -0.1 5,423,804 - +0.5 5,423,804 - -1.1 5,423,804 - -0.8 5,423,815 + +0.3 5,423,816 - -0.8 5,423,866 - -0.5 5,423,878 + -1.7 5,423,878 + -0.3 5,423,878 + -1.7 5,423,892 + -2.6 5,423,893 - +0.5 5,423,893 - +0.5 5,423,893 - -1.5 5,423,893 - +0.5 5,424,066 - Pf6N2E2_4690 0.11 -0.5 5,424,066 - Pf6N2E2_4690 0.11 -1.3 5,424,066 - Pf6N2E2_4690 0.11 -1.5 5,424,142 + Pf6N2E2_4690 0.18 -0.8 5,424,142 + Pf6N2E2_4690 0.18 -0.5 5,424,142 + Pf6N2E2_4690 0.18 +0.5 5,424,143 - Pf6N2E2_4690 0.19 -0.5 5,424,143 - Pf6N2E2_4690 0.19 -0.2 5,424,220 + Pf6N2E2_4690 0.27 -1.1 5,424,220 + Pf6N2E2_4690 0.27 +0.1 5,424,220 + Pf6N2E2_4690 0.27 -1.1 5,424,221 - Pf6N2E2_4690 0.27 +0.5 5,424,221 - Pf6N2E2_4690 0.27 -0.5 5,424,221 - Pf6N2E2_4690 0.27 +0.5
Or see this region's nucleotide sequence