Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_453

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_452 and Pf6N2E2_453 are separated by 105 nucleotidesPf6N2E2_453 and Pf6N2E2_454 are separated by 379 nucleotides Pf6N2E2_452 - Rtn protein, at 513,969 to 515,513 _452 Pf6N2E2_453 - LysR family transcriptional regulator STM2281, at 515,619 to 516,482 _453 Pf6N2E2_454 - Mobile element protein, at 516,862 to 518,130 _454 Position (kb) 515 516 517Strain fitness (log2 ratio) -1 0 1at 514.668 kb on + strand, within Pf6N2E2_452at 514.685 kb on - strand, within Pf6N2E2_452at 514.685 kb on - strand, within Pf6N2E2_452at 514.685 kb on - strand, within Pf6N2E2_452at 514.831 kb on + strand, within Pf6N2E2_452at 514.831 kb on + strand, within Pf6N2E2_452at 514.866 kb on + strand, within Pf6N2E2_452at 514.866 kb on + strand, within Pf6N2E2_452at 514.867 kb on - strand, within Pf6N2E2_452at 514.867 kb on - strand, within Pf6N2E2_452at 515.194 kb on + strand, within Pf6N2E2_452at 515.195 kb on - strand, within Pf6N2E2_452at 515.195 kb on - strand, within Pf6N2E2_452at 515.195 kb on - strand, within Pf6N2E2_452at 515.195 kb on - strand, within Pf6N2E2_452at 515.195 kb on - strand, within Pf6N2E2_452at 515.195 kb on - strand, within Pf6N2E2_452at 515.213 kb on + strand, within Pf6N2E2_452at 515.214 kb on - strand, within Pf6N2E2_452at 515.214 kb on - strand, within Pf6N2E2_452at 515.214 kb on - strand, within Pf6N2E2_452at 515.214 kb on - strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strand, within Pf6N2E2_452at 515.304 kb on + strandat 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.305 kb on - strand, within Pf6N2E2_452at 515.413 kb on + strandat 515.413 kb on + strandat 515.414 kb on - strandat 515.442 kb on + strandat 515.569 kb on + strandat 515.569 kb on + strandat 515.569 kb on + strandat 515.569 kb on + strandat 515.569 kb on + strandat 515.569 kb on + strandat 515.570 kb on - strandat 515.570 kb on - strandat 515.972 kb on + strand, within Pf6N2E2_453at 515.973 kb on - strand, within Pf6N2E2_453at 516.017 kb on + strand, within Pf6N2E2_453at 516.508 kb on + strandat 516.606 kb on - strandat 516.606 kb on - strandat 516.610 kb on - strandat 516.610 kb on - strandat 516.610 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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514,668 + Pf6N2E2_452 0.45 -1.0
514,685 - Pf6N2E2_452 0.46 +0.3
514,685 - Pf6N2E2_452 0.46 +0.3
514,685 - Pf6N2E2_452 0.46 +0.3
514,831 + Pf6N2E2_452 0.56 +0.3
514,831 + Pf6N2E2_452 0.56 +0.3
514,866 + Pf6N2E2_452 0.58 +0.3
514,866 + Pf6N2E2_452 0.58 -0.8
514,867 - Pf6N2E2_452 0.58 +0.3
514,867 - Pf6N2E2_452 0.58 +0.3
515,194 + Pf6N2E2_452 0.79 +0.7
515,195 - Pf6N2E2_452 0.79 -1.5
515,195 - Pf6N2E2_452 0.79 +0.3
515,195 - Pf6N2E2_452 0.79 +0.3
515,195 - Pf6N2E2_452 0.79 -0.1
515,195 - Pf6N2E2_452 0.79 -1.3
515,195 - Pf6N2E2_452 0.79 -1.1
515,213 + Pf6N2E2_452 0.81 -1.7
515,214 - Pf6N2E2_452 0.81 -0.8
515,214 - Pf6N2E2_452 0.81 -0.7
515,214 - Pf6N2E2_452 0.81 +0.3
515,214 - Pf6N2E2_452 0.81 +0.3
515,304 + Pf6N2E2_452 0.86 -1.4
515,304 + Pf6N2E2_452 0.86 -0.9
515,304 + Pf6N2E2_452 0.86 +0.3
515,304 + Pf6N2E2_452 0.86 +0.3
515,304 + Pf6N2E2_452 0.86 -0.4
515,304 + Pf6N2E2_452 0.86 -1.1
515,304 + Pf6N2E2_452 0.86 +0.3
515,304 + +0.3
515,305 - Pf6N2E2_452 0.86 -1.3
515,305 - Pf6N2E2_452 0.86 +1.0
515,305 - Pf6N2E2_452 0.86 +0.3
515,305 - Pf6N2E2_452 0.86 +0.3
515,305 - Pf6N2E2_452 0.86 +0.3
515,305 - Pf6N2E2_452 0.86 -0.4
515,305 - Pf6N2E2_452 0.86 +0.3
515,305 - Pf6N2E2_452 0.86 -0.7
515,413 + +0.3
515,413 + -0.9
515,414 - -0.9
515,442 + +0.3
515,569 + -0.7
515,569 + +0.3
515,569 + +0.3
515,569 + -0.7
515,569 + +0.3
515,569 + -1.3
515,570 - -0.7
515,570 - +0.3
515,972 + Pf6N2E2_453 0.41 -0.7
515,973 - Pf6N2E2_453 0.41 -1.7
516,017 + Pf6N2E2_453 0.46 +0.3
516,508 + -0.5
516,606 - +0.3
516,606 - -0.7
516,610 - -1.7
516,610 - +0.3
516,610 - +0.3

Or see this region's nucleotide sequence