Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4392

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4391 and Pf6N2E2_4392 overlap by 29 nucleotidesPf6N2E2_4392 and Pf6N2E2_4393 are separated by 97 nucleotides Pf6N2E2_4391 - D-amino acid dehydrogenase small subunit (EC 1.4.99.1), at 5,119,527 to 5,120,831 _4391 Pf6N2E2_4392 - Translation initiation inhibitor, at 5,120,803 to 5,121,156 _4392 Pf6N2E2_4393 - Alanine racemase (EC 5.1.1.1), at 5,121,254 to 5,122,327 _4393 Position (kb) 5120 5121 5122Strain fitness (log2 ratio) -3 -2 -1 0 1at 5119.867 kb on - strand, within Pf6N2E2_4391at 5119.914 kb on + strand, within Pf6N2E2_4391at 5119.914 kb on + strand, within Pf6N2E2_4391at 5120.023 kb on + strand, within Pf6N2E2_4391at 5120.135 kb on + strand, within Pf6N2E2_4391at 5120.292 kb on + strand, within Pf6N2E2_4391at 5120.352 kb on + strand, within Pf6N2E2_4391at 5120.352 kb on + strand, within Pf6N2E2_4391at 5120.418 kb on + strand, within Pf6N2E2_4391at 5120.418 kb on + strand, within Pf6N2E2_4391at 5120.418 kb on + strand, within Pf6N2E2_4391at 5120.418 kb on + strand, within Pf6N2E2_4391at 5120.419 kb on - strand, within Pf6N2E2_4391at 5120.419 kb on - strand, within Pf6N2E2_4391at 5120.419 kb on - strand, within Pf6N2E2_4391at 5120.419 kb on - strand, within Pf6N2E2_4391at 5120.419 kb on - strand, within Pf6N2E2_4391at 5120.420 kb on + strand, within Pf6N2E2_4391at 5120.420 kb on + strand, within Pf6N2E2_4391at 5120.762 kb on + strandat 5120.762 kb on + strandat 5120.762 kb on + strandat 5120.763 kb on - strandat 5120.869 kb on + strand, within Pf6N2E2_4392at 5121.001 kb on + strand, within Pf6N2E2_4392at 5121.154 kb on + strandat 5121.158 kb on + strandat 5121.158 kb on + strandat 5121.158 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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5,119,867 - Pf6N2E2_4391 0.26 +0.5
5,119,914 + Pf6N2E2_4391 0.30 +0.5
5,119,914 + Pf6N2E2_4391 0.30 +0.5
5,120,023 + Pf6N2E2_4391 0.38 +0.5
5,120,135 + Pf6N2E2_4391 0.47 +0.5
5,120,292 + Pf6N2E2_4391 0.59 +0.5
5,120,352 + Pf6N2E2_4391 0.63 +0.5
5,120,352 + Pf6N2E2_4391 0.63 +0.5
5,120,418 + Pf6N2E2_4391 0.68 +0.5
5,120,418 + Pf6N2E2_4391 0.68 +0.5
5,120,418 + Pf6N2E2_4391 0.68 -1.8
5,120,418 + Pf6N2E2_4391 0.68 +0.5
5,120,419 - Pf6N2E2_4391 0.68 -0.5
5,120,419 - Pf6N2E2_4391 0.68 +0.5
5,120,419 - Pf6N2E2_4391 0.68 +0.5
5,120,419 - Pf6N2E2_4391 0.68 -0.5
5,120,419 - Pf6N2E2_4391 0.68 +0.5
5,120,420 + Pf6N2E2_4391 0.68 +0.5
5,120,420 + Pf6N2E2_4391 0.68 -0.5
5,120,762 + -0.2
5,120,762 + -2.5
5,120,762 + -0.5
5,120,763 - +0.5
5,120,869 + Pf6N2E2_4392 0.19 -0.5
5,121,001 + Pf6N2E2_4392 0.56 -1.5
5,121,154 + +0.5
5,121,158 + -0.5
5,121,158 + -0.5
5,121,158 + -3.2

Or see this region's nucleotide sequence