Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4350 and Pf6N2E2_4351 are separated by 308 nucleotides Pf6N2E2_4351 and Pf6N2E2_4352 are separated by 148 nucleotides
Pf6N2E2_4350 - RTX toxins and related Ca2+-binding proteins, at 5,078,885 to 5,080,228
_4350
Pf6N2E2_4351 - Predicted signal transduction protein, at 5,080,537 to 5,081,868
_4351
Pf6N2E2_4352 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 5,082,017 to 5,084,092
_4352
Position (kb) 5080
5081
5082 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 5079.550 kb on + strand, within Pf6N2E2_4350 at 5079.550 kb on + strand, within Pf6N2E2_4350 at 5079.551 kb on - strand, within Pf6N2E2_4350 at 5079.551 kb on - strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.672 kb on - strand, within Pf6N2E2_4350 at 5079.672 kb on - strand, within Pf6N2E2_4350 at 5079.672 kb on - strand, within Pf6N2E2_4350 at 5079.766 kb on + strand, within Pf6N2E2_4350 at 5079.767 kb on - strand, within Pf6N2E2_4350 at 5079.801 kb on + strand, within Pf6N2E2_4350 at 5079.801 kb on + strand, within Pf6N2E2_4350 at 5079.852 kb on + strand, within Pf6N2E2_4350 at 5079.927 kb on + strand, within Pf6N2E2_4350 at 5079.927 kb on + strand, within Pf6N2E2_4350 at 5079.927 kb on + strand, within Pf6N2E2_4350 at 5079.940 kb on + strand, within Pf6N2E2_4350 at 5079.940 kb on + strand, within Pf6N2E2_4350 at 5079.941 kb on - strand, within Pf6N2E2_4350 at 5079.996 kb on - strand, within Pf6N2E2_4350 at 5080.012 kb on + strand, within Pf6N2E2_4350 at 5080.012 kb on + strand, within Pf6N2E2_4350 at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.128 kb on + strand at 5080.128 kb on + strand at 5080.128 kb on + strand at 5080.129 kb on - strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.192 kb on + strand at 5080.192 kb on + strand at 5080.193 kb on - strand at 5080.217 kb on + strand at 5080.226 kb on + strand at 5080.244 kb on + strand at 5080.244 kb on + strand at 5080.244 kb on + strand at 5080.245 kb on - strand at 5080.245 kb on - strand at 5080.245 kb on - strand at 5080.245 kb on - strand at 5080.344 kb on + strand at 5080.344 kb on + strand at 5080.345 kb on - strand at 5080.633 kb on + strand at 5080.634 kb on - strand at 5080.634 kb on - strand at 5080.634 kb on - strand at 5080.706 kb on - strand, within Pf6N2E2_4351 at 5080.740 kb on - strand, within Pf6N2E2_4351 at 5080.868 kb on + strand, within Pf6N2E2_4351 at 5080.868 kb on + strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.962 kb on + strand, within Pf6N2E2_4351 at 5080.963 kb on - strand, within Pf6N2E2_4351 at 5080.982 kb on + strand, within Pf6N2E2_4351 at 5080.982 kb on + strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5081.054 kb on + strand, within Pf6N2E2_4351 at 5081.054 kb on + strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.072 kb on + strand, within Pf6N2E2_4351 at 5081.216 kb on + strand, within Pf6N2E2_4351 at 5081.216 kb on + strand, within Pf6N2E2_4351 at 5081.219 kb on + strand, within Pf6N2E2_4351 at 5081.606 kb on + strand, within Pf6N2E2_4351 at 5081.607 kb on - strand, within Pf6N2E2_4351 at 5081.809 kb on + strand at 5081.809 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.972 kb on + strand at 5081.973 kb on - strand at 5082.777 kb on + strand, within Pf6N2E2_4352
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 5,079,550 + Pf6N2E2_4350 0.49 -1.1 5,079,550 + Pf6N2E2_4350 0.49 +0.5 5,079,551 - Pf6N2E2_4350 0.50 +0.5 5,079,551 - Pf6N2E2_4350 0.50 +0.5 5,079,671 + Pf6N2E2_4350 0.58 -0.4 5,079,671 + Pf6N2E2_4350 0.58 -1.8 5,079,671 + Pf6N2E2_4350 0.58 +0.5 5,079,671 + Pf6N2E2_4350 0.58 -0.3 5,079,671 + Pf6N2E2_4350 0.58 -0.5 5,079,672 - Pf6N2E2_4350 0.59 +0.5 5,079,672 - Pf6N2E2_4350 0.59 +0.5 5,079,672 - Pf6N2E2_4350 0.59 +0.5 5,079,766 + Pf6N2E2_4350 0.66 -0.9 5,079,767 - Pf6N2E2_4350 0.66 -1.1 5,079,801 + Pf6N2E2_4350 0.68 -0.5 5,079,801 + Pf6N2E2_4350 0.68 +0.5 5,079,852 + Pf6N2E2_4350 0.72 +0.5 5,079,927 + Pf6N2E2_4350 0.78 +0.5 5,079,927 + Pf6N2E2_4350 0.78 -1.1 5,079,927 + Pf6N2E2_4350 0.78 -0.8 5,079,940 + Pf6N2E2_4350 0.78 +0.5 5,079,940 + Pf6N2E2_4350 0.78 -1.5 5,079,941 - Pf6N2E2_4350 0.79 +0.5 5,079,996 - Pf6N2E2_4350 0.83 +0.5 5,080,012 + Pf6N2E2_4350 0.84 +0.5 5,080,012 + Pf6N2E2_4350 0.84 -0.2 5,080,103 + -0.5 5,080,103 + -1.1 5,080,103 + -1.5 5,080,103 + -0.5 5,080,103 + -1.5 5,080,103 + -0.2 5,080,104 - +0.5 5,080,104 - -1.5 5,080,104 - -0.5 5,080,104 - -0.5 5,080,104 - -0.5 5,080,104 - -0.5 5,080,104 - -0.5 5,080,104 - -0.4 5,080,104 - +0.5 5,080,128 + -0.1 5,080,128 + -2.1 5,080,128 + -1.1 5,080,129 - -0.5 5,080,190 + -1.3 5,080,190 + -2.1 5,080,190 + -1.1 5,080,190 + -1.2 5,080,190 + +0.5 5,080,190 + +0.5 5,080,190 + -1.1 5,080,190 + -3.1 5,080,191 - +0.5 5,080,191 - -0.5 5,080,191 - +0.5 5,080,191 - +0.5 5,080,191 - +0.5 5,080,191 - +0.5 5,080,191 - -2.2 5,080,191 - +0.2 5,080,192 + -0.5 5,080,192 + -0.5 5,080,193 - +0.5 5,080,217 + -0.5 5,080,226 + +0.5 5,080,244 + +0.5 5,080,244 + -0.5 5,080,244 + +0.2 5,080,245 - -0.5 5,080,245 - +1.5 5,080,245 - -0.5 5,080,245 - -0.5 5,080,344 + -0.6 5,080,344 + +0.5 5,080,345 - +0.5 5,080,633 + -1.1 5,080,634 - +0.5 5,080,634 - -1.1 5,080,634 - -3.4 5,080,706 - Pf6N2E2_4351 0.13 -3.7 5,080,740 - Pf6N2E2_4351 0.15 -0.5 5,080,868 + Pf6N2E2_4351 0.25 -4.8 5,080,868 + Pf6N2E2_4351 0.25 -2.8 5,080,869 - Pf6N2E2_4351 0.25 -3.5 5,080,869 - Pf6N2E2_4351 0.25 -1.1 5,080,869 - Pf6N2E2_4351 0.25 -2.1 5,080,869 - Pf6N2E2_4351 0.25 -1.2 5,080,869 - Pf6N2E2_4351 0.25 +0.5 5,080,869 - Pf6N2E2_4351 0.25 -3.8 5,080,962 + Pf6N2E2_4351 0.32 -2.5 5,080,963 - Pf6N2E2_4351 0.32 -1.3 5,080,982 + Pf6N2E2_4351 0.33 +0.5 5,080,982 + Pf6N2E2_4351 0.33 -3.2 5,080,983 - Pf6N2E2_4351 0.33 +0.5 5,080,983 - Pf6N2E2_4351 0.33 -3.2 5,080,983 - Pf6N2E2_4351 0.33 -1.1 5,080,983 - Pf6N2E2_4351 0.33 -0.8 5,080,983 - Pf6N2E2_4351 0.33 +0.5 5,081,054 + Pf6N2E2_4351 0.39 -2.5 5,081,054 + Pf6N2E2_4351 0.39 -1.5 5,081,055 - Pf6N2E2_4351 0.39 +0.5 5,081,055 - Pf6N2E2_4351 0.39 -3.5 5,081,055 - Pf6N2E2_4351 0.39 -3.7 5,081,055 - Pf6N2E2_4351 0.39 -1.1 5,081,055 - Pf6N2E2_4351 0.39 -2.8 5,081,055 - Pf6N2E2_4351 0.39 -2.4 5,081,055 - Pf6N2E2_4351 0.39 +0.5 5,081,055 - Pf6N2E2_4351 0.39 -2.3 5,081,072 + Pf6N2E2_4351 0.40 -2.5 5,081,216 + Pf6N2E2_4351 0.51 -2.3 5,081,216 + Pf6N2E2_4351 0.51 -1.9 5,081,219 + Pf6N2E2_4351 0.51 -4.4 5,081,606 + Pf6N2E2_4351 0.80 -2.6 5,081,607 - Pf6N2E2_4351 0.80 -3.7 5,081,809 + -3.3 5,081,809 + -1.1 5,081,970 + +0.5 5,081,970 + -0.5 5,081,970 + +0.5 5,081,970 + +0.5 5,081,970 + -0.5 5,081,970 + -3.6 5,081,971 - +0.5 5,081,971 - -1.1 5,081,971 - +0.5 5,081,971 - -0.5 5,081,972 + +0.5 5,081,973 - +0.5 5,082,777 + Pf6N2E2_4352 0.37 +0.5
Or see this region's nucleotide sequence