Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4073 and Pf6N2E2_4074 are separated by 15 nucleotides Pf6N2E2_4074 and Pf6N2E2_4075 are separated by 147 nucleotides
Pf6N2E2_4073 - Phosphate transport system permease protein PstA (TC 3.A.1.7.1), at 4,748,418 to 4,750,088
_4073
Pf6N2E2_4074 - Phosphate transport system permease protein PstC (TC 3.A.1.7.1), at 4,750,104 to 4,752,395
_4074
Pf6N2E2_4075 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 4,752,543 to 4,753,541
_4075
Position (kb)
4750
4751
4752
4753 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4749.241 kb on + strand, within Pf6N2E2_4073 at 4749.242 kb on - strand, within Pf6N2E2_4073 at 4749.242 kb on - strand, within Pf6N2E2_4073 at 4749.283 kb on + strand, within Pf6N2E2_4073 at 4749.404 kb on - strand, within Pf6N2E2_4073 at 4749.582 kb on + strand, within Pf6N2E2_4073 at 4749.658 kb on + strand, within Pf6N2E2_4073 at 4749.665 kb on - strand, within Pf6N2E2_4073 at 4749.922 kb on + strand at 4749.923 kb on - strand at 4749.923 kb on - strand at 4749.923 kb on - strand at 4750.096 kb on + strand at 4750.096 kb on + strand at 4750.097 kb on - strand at 4750.097 kb on - strand at 4750.097 kb on - strand at 4750.097 kb on - strand at 4750.097 kb on - strand at 4750.097 kb on - strand at 4750.105 kb on + strand at 4750.106 kb on - strand at 4750.106 kb on - strand at 4750.118 kb on - strand at 4750.507 kb on + strand, within Pf6N2E2_4074 at 4750.507 kb on + strand, within Pf6N2E2_4074 at 4750.507 kb on + strand, within Pf6N2E2_4074 at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.508 kb on - strand at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.508 kb on - strand, within Pf6N2E2_4074 at 4750.636 kb on + strand, within Pf6N2E2_4074 at 4750.636 kb on + strand, within Pf6N2E2_4074 at 4750.648 kb on + strand, within Pf6N2E2_4074 at 4750.648 kb on + strand, within Pf6N2E2_4074 at 4750.649 kb on - strand, within Pf6N2E2_4074 at 4750.649 kb on - strand, within Pf6N2E2_4074 at 4750.649 kb on - strand, within Pf6N2E2_4074 at 4750.649 kb on - strand at 4750.649 kb on - strand, within Pf6N2E2_4074 at 4750.649 kb on - strand, within Pf6N2E2_4074 at 4750.649 kb on - strand, within Pf6N2E2_4074 at 4750.933 kb on + strand, within Pf6N2E2_4074 at 4750.934 kb on - strand, within Pf6N2E2_4074 at 4750.942 kb on + strand, within Pf6N2E2_4074 at 4750.942 kb on + strand, within Pf6N2E2_4074 at 4750.943 kb on - strand, within Pf6N2E2_4074 at 4750.943 kb on - strand, within Pf6N2E2_4074 at 4750.987 kb on + strand, within Pf6N2E2_4074 at 4750.988 kb on - strand, within Pf6N2E2_4074 at 4750.988 kb on - strand, within Pf6N2E2_4074 at 4751.077 kb on + strand, within Pf6N2E2_4074 at 4751.077 kb on + strand, within Pf6N2E2_4074 at 4751.077 kb on + strand, within Pf6N2E2_4074 at 4751.077 kb on + strand, within Pf6N2E2_4074 at 4751.077 kb on + strand, within Pf6N2E2_4074 at 4751.078 kb on - strand, within Pf6N2E2_4074 at 4751.078 kb on - strand, within Pf6N2E2_4074 at 4751.078 kb on - strand, within Pf6N2E2_4074 at 4751.078 kb on - strand, within Pf6N2E2_4074 at 4751.078 kb on - strand, within Pf6N2E2_4074 at 4751.078 kb on - strand, within Pf6N2E2_4074 at 4751.093 kb on - strand, within Pf6N2E2_4074 at 4751.261 kb on + strand, within Pf6N2E2_4074 at 4751.262 kb on - strand, within Pf6N2E2_4074 at 4751.262 kb on - strand, within Pf6N2E2_4074 at 4751.262 kb on - strand, within Pf6N2E2_4074 at 4751.378 kb on + strand, within Pf6N2E2_4074 at 4751.378 kb on + strand, within Pf6N2E2_4074 at 4751.378 kb on + strand, within Pf6N2E2_4074 at 4751.378 kb on + strand, within Pf6N2E2_4074 at 4751.379 kb on - strand, within Pf6N2E2_4074 at 4751.379 kb on - strand, within Pf6N2E2_4074 at 4751.530 kb on + strand, within Pf6N2E2_4074 at 4751.531 kb on - strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.596 kb on + strand, within Pf6N2E2_4074 at 4751.597 kb on - strand, within Pf6N2E2_4074 at 4751.597 kb on - strand, within Pf6N2E2_4074 at 4751.597 kb on - strand, within Pf6N2E2_4074 at 4751.597 kb on - strand, within Pf6N2E2_4074 at 4751.840 kb on - strand, within Pf6N2E2_4074 at 4751.845 kb on + strand, within Pf6N2E2_4074 at 4751.845 kb on + strand, within Pf6N2E2_4074 at 4751.895 kb on - strand, within Pf6N2E2_4074 at 4751.896 kb on + strand, within Pf6N2E2_4074 at 4751.896 kb on + strand, within Pf6N2E2_4074 at 4751.896 kb on + strand, within Pf6N2E2_4074 at 4751.896 kb on + strand, within Pf6N2E2_4074 at 4751.896 kb on + strand, within Pf6N2E2_4074 at 4751.896 kb on + strand, within Pf6N2E2_4074 at 4751.897 kb on - strand, within Pf6N2E2_4074 at 4751.897 kb on - strand, within Pf6N2E2_4074 at 4751.897 kb on - strand, within Pf6N2E2_4074 at 4751.897 kb on - strand, within Pf6N2E2_4074 at 4751.897 kb on - strand, within Pf6N2E2_4074 at 4751.910 kb on + strand, within Pf6N2E2_4074 at 4751.910 kb on + strand, within Pf6N2E2_4074 at 4752.202 kb on + strand at 4752.202 kb on + strand at 4752.202 kb on + strand at 4752.227 kb on + strand at 4752.228 kb on - strand at 4752.228 kb on - strand at 4752.265 kb on + strand at 4752.266 kb on - strand at 4752.300 kb on - strand at 4752.327 kb on - strand at 4752.359 kb on + strand at 4752.360 kb on - strand at 4752.411 kb on + strand at 4752.541 kb on + strand at 4752.541 kb on + strand at 4752.542 kb on - strand at 4752.542 kb on - strand at 4752.544 kb on + strand at 4752.544 kb on + strand at 4752.544 kb on + strand at 4752.545 kb on - strand at 4752.545 kb on - strand at 4752.545 kb on - strand at 4752.626 kb on - strand at 4752.626 kb on - strand at 4752.733 kb on + strand, within Pf6N2E2_4075 at 4752.734 kb on - strand, within Pf6N2E2_4075 at 4752.754 kb on + strand, within Pf6N2E2_4075 at 4752.754 kb on + strand, within Pf6N2E2_4075 at 4752.898 kb on - strand, within Pf6N2E2_4075 at 4752.940 kb on + strand, within Pf6N2E2_4075 at 4752.941 kb on - strand, within Pf6N2E2_4075 at 4752.941 kb on - strand, within Pf6N2E2_4075 at 4752.979 kb on + strand, within Pf6N2E2_4075 at 4753.094 kb on + strand, within Pf6N2E2_4075 at 4753.156 kb on - strand, within Pf6N2E2_4075 at 4753.156 kb on - strand, within Pf6N2E2_4075 at 4753.205 kb on - strand, within Pf6N2E2_4075 at 4753.335 kb on + strand, within Pf6N2E2_4075 at 4753.335 kb on + strand, within Pf6N2E2_4075 at 4753.335 kb on + strand, within Pf6N2E2_4075 at 4753.336 kb on - strand, within Pf6N2E2_4075 at 4753.357 kb on + strand, within Pf6N2E2_4075 at 4753.358 kb on - strand, within Pf6N2E2_4075
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 4,749,241 + Pf6N2E2_4073 0.49 -1.8 4,749,242 - Pf6N2E2_4073 0.49 -0.1 4,749,242 - Pf6N2E2_4073 0.49 -1.1 4,749,283 + Pf6N2E2_4073 0.52 -2.4 4,749,404 - Pf6N2E2_4073 0.59 -1.1 4,749,582 + Pf6N2E2_4073 0.70 +1.5 4,749,658 + Pf6N2E2_4073 0.74 +0.5 4,749,665 - Pf6N2E2_4073 0.75 -1.8 4,749,922 + -3.0 4,749,923 - -3.1 4,749,923 - +1.5 4,749,923 - -0.9 4,750,096 + +0.5 4,750,096 + -2.0 4,750,097 - -0.7 4,750,097 - -0.5 4,750,097 - +0.5 4,750,097 - -0.4 4,750,097 - -0.7 4,750,097 - -0.5 4,750,105 + +0.5 4,750,106 - +0.5 4,750,106 - -1.8 4,750,118 - -0.5 4,750,507 + Pf6N2E2_4074 0.18 -1.8 4,750,507 + Pf6N2E2_4074 0.18 -2.4 4,750,507 + Pf6N2E2_4074 0.18 -2.1 4,750,508 - Pf6N2E2_4074 0.18 -1.5 4,750,508 - -0.3 4,750,508 - Pf6N2E2_4074 0.18 -1.8 4,750,508 - Pf6N2E2_4074 0.18 -2.3 4,750,508 - Pf6N2E2_4074 0.18 -1.0 4,750,508 - Pf6N2E2_4074 0.18 -2.2 4,750,508 - Pf6N2E2_4074 0.18 -0.8 4,750,508 - Pf6N2E2_4074 0.18 -1.4 4,750,636 + Pf6N2E2_4074 0.23 -1.9 4,750,636 + Pf6N2E2_4074 0.23 -5.4 4,750,648 + Pf6N2E2_4074 0.24 -2.5 4,750,648 + Pf6N2E2_4074 0.24 -2.3 4,750,649 - Pf6N2E2_4074 0.24 -0.6 4,750,649 - Pf6N2E2_4074 0.24 -1.2 4,750,649 - Pf6N2E2_4074 0.24 -1.6 4,750,649 - -2.3 4,750,649 - Pf6N2E2_4074 0.24 -1.7 4,750,649 - Pf6N2E2_4074 0.24 -1.0 4,750,649 - Pf6N2E2_4074 0.24 -1.5 4,750,933 + Pf6N2E2_4074 0.36 +0.5 4,750,934 - Pf6N2E2_4074 0.36 -1.8 4,750,942 + Pf6N2E2_4074 0.37 -1.5 4,750,942 + Pf6N2E2_4074 0.37 -2.0 4,750,943 - Pf6N2E2_4074 0.37 -1.2 4,750,943 - Pf6N2E2_4074 0.37 -1.1 4,750,987 + Pf6N2E2_4074 0.39 -1.8 4,750,988 - Pf6N2E2_4074 0.39 +0.5 4,750,988 - Pf6N2E2_4074 0.39 -2.2 4,751,077 + Pf6N2E2_4074 0.42 -1.5 4,751,077 + Pf6N2E2_4074 0.42 -2.0 4,751,077 + Pf6N2E2_4074 0.42 -2.1 4,751,077 + Pf6N2E2_4074 0.42 -2.5 4,751,077 + Pf6N2E2_4074 0.42 -1.8 4,751,078 - Pf6N2E2_4074 0.42 -1.5 4,751,078 - Pf6N2E2_4074 0.42 -0.7 4,751,078 - Pf6N2E2_4074 0.42 -0.4 4,751,078 - Pf6N2E2_4074 0.42 -1.1 4,751,078 - Pf6N2E2_4074 0.42 -1.2 4,751,078 - Pf6N2E2_4074 0.42 -1.1 4,751,093 - Pf6N2E2_4074 0.43 -1.6 4,751,261 + Pf6N2E2_4074 0.50 -1.7 4,751,262 - Pf6N2E2_4074 0.51 -1.7 4,751,262 - Pf6N2E2_4074 0.51 -1.7 4,751,262 - Pf6N2E2_4074 0.51 -1.8 4,751,378 + Pf6N2E2_4074 0.56 -1.9 4,751,378 + Pf6N2E2_4074 0.56 -1.3 4,751,378 + Pf6N2E2_4074 0.56 -1.6 4,751,378 + Pf6N2E2_4074 0.56 -2.6 4,751,379 - Pf6N2E2_4074 0.56 -1.8 4,751,379 - Pf6N2E2_4074 0.56 -2.4 4,751,530 + Pf6N2E2_4074 0.62 -2.0 4,751,531 - Pf6N2E2_4074 0.62 -2.6 4,751,596 + Pf6N2E2_4074 0.65 -1.4 4,751,596 + Pf6N2E2_4074 0.65 -2.0 4,751,596 + Pf6N2E2_4074 0.65 -1.5 4,751,596 + Pf6N2E2_4074 0.65 +0.5 4,751,596 + Pf6N2E2_4074 0.65 -2.2 4,751,596 + Pf6N2E2_4074 0.65 -2.1 4,751,596 + Pf6N2E2_4074 0.65 -2.4 4,751,596 + Pf6N2E2_4074 0.65 -1.7 4,751,596 + Pf6N2E2_4074 0.65 -2.9 4,751,597 - Pf6N2E2_4074 0.65 -2.3 4,751,597 - Pf6N2E2_4074 0.65 -0.5 4,751,597 - Pf6N2E2_4074 0.65 -1.3 4,751,597 - Pf6N2E2_4074 0.65 -2.1 4,751,840 - Pf6N2E2_4074 0.76 -1.8 4,751,845 + Pf6N2E2_4074 0.76 -2.5 4,751,845 + Pf6N2E2_4074 0.76 -1.8 4,751,895 - Pf6N2E2_4074 0.78 -2.2 4,751,896 + Pf6N2E2_4074 0.78 -2.3 4,751,896 + Pf6N2E2_4074 0.78 -1.6 4,751,896 + Pf6N2E2_4074 0.78 -2.9 4,751,896 + Pf6N2E2_4074 0.78 -2.4 4,751,896 + Pf6N2E2_4074 0.78 -1.1 4,751,896 + Pf6N2E2_4074 0.78 -1.9 4,751,897 - Pf6N2E2_4074 0.78 -0.7 4,751,897 - Pf6N2E2_4074 0.78 -3.4 4,751,897 - Pf6N2E2_4074 0.78 -2.1 4,751,897 - Pf6N2E2_4074 0.78 -1.9 4,751,897 - Pf6N2E2_4074 0.78 -1.1 4,751,910 + Pf6N2E2_4074 0.79 -1.0 4,751,910 + Pf6N2E2_4074 0.79 -1.9 4,752,202 + -2.2 4,752,202 + -1.6 4,752,202 + -2.1 4,752,227 + -1.5 4,752,228 - -2.5 4,752,228 - -2.2 4,752,265 + -2.3 4,752,266 - -1.9 4,752,300 - -1.5 4,752,327 - -0.5 4,752,359 + -1.7 4,752,360 - -1.5 4,752,411 + -0.5 4,752,541 + -0.5 4,752,541 + -0.4 4,752,542 - -0.6 4,752,542 - -1.1 4,752,544 + -1.2 4,752,544 + -0.5 4,752,544 + -0.9 4,752,545 - -0.7 4,752,545 - +0.5 4,752,545 - -0.5 4,752,626 - -1.6 4,752,626 - +0.5 4,752,733 + Pf6N2E2_4075 0.19 -1.1 4,752,734 - Pf6N2E2_4075 0.19 -2.9 4,752,754 + Pf6N2E2_4075 0.21 -2.4 4,752,754 + Pf6N2E2_4075 0.21 -0.7 4,752,898 - Pf6N2E2_4075 0.36 -2.0 4,752,940 + Pf6N2E2_4075 0.40 -2.7 4,752,941 - Pf6N2E2_4075 0.40 -2.1 4,752,941 - Pf6N2E2_4075 0.40 -2.1 4,752,979 + Pf6N2E2_4075 0.44 -2.5 4,753,094 + Pf6N2E2_4075 0.55 -1.5 4,753,156 - Pf6N2E2_4075 0.61 -1.5 4,753,156 - Pf6N2E2_4075 0.61 -3.2 4,753,205 - Pf6N2E2_4075 0.66 -2.7 4,753,335 + Pf6N2E2_4075 0.79 -3.0 4,753,335 + Pf6N2E2_4075 0.79 -3.0 4,753,335 + Pf6N2E2_4075 0.79 -0.5 4,753,336 - Pf6N2E2_4075 0.79 -2.8 4,753,357 + Pf6N2E2_4075 0.81 -2.9 4,753,358 - Pf6N2E2_4075 0.82 -2.1
Or see this region's nucleotide sequence