Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_394 and Pf6N2E2_395 are separated by 337 nucleotides Pf6N2E2_395 and Pf6N2E2_396 are separated by 684 nucleotides
Pf6N2E2_394 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 462,283 to 463,059
_394
Pf6N2E2_395 - Glutathione S-transferase (EC 2.5.1.18), at 463,397 to 463,828
_395
Pf6N2E2_396 - hypothetical protein, at 464,513 to 464,920
_396
Position (kb)
463
464 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 462.648 kb on + strand, within Pf6N2E2_394 at 462.720 kb on + strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.721 kb on - strand, within Pf6N2E2_394 at 462.731 kb on - strand, within Pf6N2E2_394 at 462.760 kb on + strand, within Pf6N2E2_394 at 462.761 kb on - strand, within Pf6N2E2_394 at 462.882 kb on + strand, within Pf6N2E2_394 at 462.890 kb on - strand, within Pf6N2E2_394 at 463.008 kb on + strand at 463.009 kb on - strand at 463.009 kb on - strand at 463.082 kb on + strand at 463.082 kb on + strand at 463.082 kb on + strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.083 kb on - strand at 463.170 kb on - strand at 463.184 kb on + strand at 463.271 kb on + strand at 463.271 kb on + strand at 463.272 kb on - strand at 463.272 kb on - strand at 463.435 kb on - strand at 463.558 kb on + strand, within Pf6N2E2_395 at 463.602 kb on + strand, within Pf6N2E2_395 at 463.603 kb on - strand, within Pf6N2E2_395 at 463.697 kb on + strand, within Pf6N2E2_395 at 463.698 kb on - strand, within Pf6N2E2_395 at 463.731 kb on - strand, within Pf6N2E2_395 at 463.795 kb on - strand at 463.795 kb on - strand at 463.882 kb on - strand at 463.924 kb on - strand at 463.957 kb on - strand at 464.009 kb on - strand at 464.009 kb on - strand at 464.475 kb on + strand at 464.476 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.508 kb on - strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.511 kb on + strand at 464.512 kb on - strand at 464.512 kb on - strand at 464.532 kb on + strand at 464.532 kb on + strand at 464.533 kb on - strand at 464.533 kb on - strand at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.594 kb on - strand, within Pf6N2E2_396 at 464.690 kb on + strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.719 kb on - strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396 at 464.812 kb on + strand, within Pf6N2E2_396
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 462,648 + Pf6N2E2_394 0.47 -0.7 462,720 + Pf6N2E2_394 0.56 +0.9 462,721 - Pf6N2E2_394 0.56 +0.3 462,721 - Pf6N2E2_394 0.56 -0.9 462,721 - Pf6N2E2_394 0.56 +0.3 462,731 - Pf6N2E2_394 0.58 +0.3 462,760 + Pf6N2E2_394 0.61 +0.3 462,761 - Pf6N2E2_394 0.62 +0.1 462,882 + Pf6N2E2_394 0.77 +0.3 462,890 - Pf6N2E2_394 0.78 +0.3 463,008 + +0.3 463,009 - -0.7 463,009 - +0.3 463,082 + +0.3 463,082 + +0.3 463,082 + -0.7 463,083 - -3.0 463,083 - -0.7 463,083 - -0.8 463,083 - -1.1 463,170 - +0.3 463,184 + +0.3 463,271 + +0.3 463,271 + -0.1 463,272 - -0.5 463,272 - -0.3 463,435 - -0.9 463,558 + Pf6N2E2_395 0.37 -0.6 463,602 + Pf6N2E2_395 0.47 -0.7 463,603 - Pf6N2E2_395 0.48 +0.3 463,697 + Pf6N2E2_395 0.69 -0.7 463,698 - Pf6N2E2_395 0.70 +1.3 463,731 - Pf6N2E2_395 0.77 +0.3 463,795 - +0.3 463,795 - +0.3 463,882 - +0.3 463,924 - +0.3 463,957 - +0.3 464,009 - +0.3 464,009 - +0.3 464,475 + -0.7 464,476 - -1.1 464,508 - -1.1 464,508 - +0.3 464,508 - -0.7 464,508 - +0.3 464,511 + -0.7 464,511 + -0.9 464,511 + +0.3 464,511 + +0.3 464,512 - -2.0 464,512 - +0.3 464,532 + +0.3 464,532 + +0.3 464,533 - -0.7 464,533 - +0.3 464,594 - Pf6N2E2_396 0.20 -0.7 464,594 - Pf6N2E2_396 0.20 +0.3 464,594 - Pf6N2E2_396 0.20 -0.9 464,690 + Pf6N2E2_396 0.43 +0.3 464,719 - Pf6N2E2_396 0.50 -0.7 464,719 - Pf6N2E2_396 0.50 -0.7 464,812 + Pf6N2E2_396 0.73 -0.7 464,812 + Pf6N2E2_396 0.73 +0.3 464,812 + Pf6N2E2_396 0.73 +0.3
Or see this region's nucleotide sequence