Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3782 and Pf6N2E2_3783 overlap by 8 nucleotides Pf6N2E2_3783 and Pf6N2E2_3784 are separated by 24 nucleotides Pf6N2E2_3784 and Pf6N2E2_3785 are separated by 10 nucleotides Pf6N2E2_3785 and Pf6N2E2_3786 overlap by 4 nucleotides Pf6N2E2_3786 and Pf6N2E2_3787 overlap by 4 nucleotides
Pf6N2E2_3782 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 4,405,518 to 4,405,916
_3782
Pf6N2E2_3783 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) , at 4,405,909 to 4,406,241
_3783
Pf6N2E2_3784 - Twin-arginine translocation protein TatA, at 4,406,266 to 4,406,553
_3784
Pf6N2E2_3785 - Twin-arginine translocation protein TatB, at 4,406,564 to 4,406,986
_3785
Pf6N2E2_3786 - Twin-arginine translocation protein TatC, at 4,406,983 to 4,407,780
_3786
Pf6N2E2_3787 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 4,407,777 to 4,408,484
_3787
Position (kb)
4406
4407 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4405.665 kb on + strand, within Pf6N2E2_3782 at 4405.665 kb on + strand, within Pf6N2E2_3782 at 4405.665 kb on + strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.666 kb on - strand, within Pf6N2E2_3782 at 4405.828 kb on - strand, within Pf6N2E2_3782 at 4405.893 kb on + strand at 4405.893 kb on + strand at 4405.894 kb on - strand at 4405.894 kb on - strand at 4405.904 kb on + strand at 4405.904 kb on + strand at 4405.904 kb on + strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.905 kb on - strand at 4405.978 kb on + strand, within Pf6N2E2_3783 at 4405.979 kb on - strand, within Pf6N2E2_3783 at 4405.979 kb on - strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.101 kb on + strand, within Pf6N2E2_3783 at 4406.102 kb on - strand, within Pf6N2E2_3783 at 4406.271 kb on + strand at 4406.562 kb on + strand at 4406.562 kb on + strand at 4406.562 kb on + strand at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.764 kb on + strand, within Pf6N2E2_3785 at 4406.765 kb on - strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.928 kb on + strand, within Pf6N2E2_3785 at 4406.962 kb on + strand at 4406.962 kb on + strand at 4406.962 kb on + strand at 4407.292 kb on + strand, within Pf6N2E2_3786 at 4407.370 kb on + strand, within Pf6N2E2_3786 at 4407.370 kb on + strand, within Pf6N2E2_3786 at 4407.373 kb on + strand, within Pf6N2E2_3786 at 4407.454 kb on + strand, within Pf6N2E2_3786 at 4407.454 kb on + strand, within Pf6N2E2_3786 at 4407.556 kb on + strand, within Pf6N2E2_3786 at 4407.577 kb on + strand, within Pf6N2E2_3786 at 4407.577 kb on + strand, within Pf6N2E2_3786 at 4407.577 kb on + strand, within Pf6N2E2_3786
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 4,405,665 + Pf6N2E2_3782 0.37 -0.5 4,405,665 + Pf6N2E2_3782 0.37 +0.5 4,405,665 + Pf6N2E2_3782 0.37 +0.5 4,405,666 - Pf6N2E2_3782 0.37 -5.0 4,405,666 - Pf6N2E2_3782 0.37 -4.4 4,405,666 - Pf6N2E2_3782 0.37 -3.7 4,405,666 - Pf6N2E2_3782 0.37 -4.0 4,405,666 - Pf6N2E2_3782 0.37 -4.2 4,405,828 - Pf6N2E2_3782 0.78 -4.0 4,405,893 + -0.5 4,405,893 + -1.0 4,405,894 - -3.5 4,405,894 - -5.0 4,405,904 + -1.1 4,405,904 + -0.5 4,405,904 + -0.4 4,405,905 - -4.6 4,405,905 - -3.5 4,405,905 - -4.8 4,405,905 - -2.7 4,405,905 - -0.5 4,405,905 - -3.4 4,405,905 - -3.7 4,405,978 + Pf6N2E2_3783 0.21 -0.3 4,405,979 - Pf6N2E2_3783 0.21 -2.1 4,405,979 - Pf6N2E2_3783 0.21 -4.1 4,406,101 + Pf6N2E2_3783 0.58 -0.5 4,406,101 + Pf6N2E2_3783 0.58 +0.5 4,406,101 + Pf6N2E2_3783 0.58 -0.4 4,406,101 + Pf6N2E2_3783 0.58 +0.3 4,406,101 + Pf6N2E2_3783 0.58 +0.5 4,406,102 - Pf6N2E2_3783 0.58 +0.5 4,406,271 + -3.4 4,406,562 + -1.8 4,406,562 + -2.7 4,406,562 + -2.2 4,406,764 + Pf6N2E2_3785 0.47 -3.3 4,406,764 + Pf6N2E2_3785 0.47 -2.9 4,406,764 + Pf6N2E2_3785 0.47 -5.1 4,406,764 + Pf6N2E2_3785 0.47 -4.5 4,406,764 + Pf6N2E2_3785 0.47 -4.7 4,406,764 + Pf6N2E2_3785 0.47 -5.6 4,406,765 - Pf6N2E2_3785 0.48 -2.5 4,406,928 + Pf6N2E2_3785 0.86 -2.5 4,406,928 + Pf6N2E2_3785 0.86 +0.5 4,406,928 + Pf6N2E2_3785 0.86 +0.5 4,406,928 + Pf6N2E2_3785 0.86 -2.3 4,406,928 + Pf6N2E2_3785 0.86 +0.5 4,406,928 + Pf6N2E2_3785 0.86 -1.5 4,406,928 + Pf6N2E2_3785 0.86 -1.1 4,406,962 + -1.1 4,406,962 + -0.3 4,406,962 + -0.5 4,407,292 + Pf6N2E2_3786 0.39 -5.6 4,407,370 + Pf6N2E2_3786 0.48 -4.8 4,407,370 + Pf6N2E2_3786 0.48 +0.5 4,407,373 + Pf6N2E2_3786 0.49 -4.3 4,407,454 + Pf6N2E2_3786 0.59 -4.3 4,407,454 + Pf6N2E2_3786 0.59 -3.2 4,407,556 + Pf6N2E2_3786 0.72 -4.1 4,407,577 + Pf6N2E2_3786 0.74 -2.5 4,407,577 + Pf6N2E2_3786 0.74 -2.5 4,407,577 + Pf6N2E2_3786 0.74 -3.9
Or see this region's nucleotide sequence