Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3785

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3782 and Pf6N2E2_3783 overlap by 8 nucleotidesPf6N2E2_3783 and Pf6N2E2_3784 are separated by 24 nucleotidesPf6N2E2_3784 and Pf6N2E2_3785 are separated by 10 nucleotidesPf6N2E2_3785 and Pf6N2E2_3786 overlap by 4 nucleotidesPf6N2E2_3786 and Pf6N2E2_3787 overlap by 4 nucleotides Pf6N2E2_3782 - Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19), at 4,405,518 to 4,405,916 _3782 Pf6N2E2_3783 - phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data), at 4,405,909 to 4,406,241 _3783 Pf6N2E2_3784 - Twin-arginine translocation protein TatA, at 4,406,266 to 4,406,553 _3784 Pf6N2E2_3785 - Twin-arginine translocation protein TatB, at 4,406,564 to 4,406,986 _3785 Pf6N2E2_3786 - Twin-arginine translocation protein TatC, at 4,406,983 to 4,407,780 _3786 Pf6N2E2_3787 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 4,407,777 to 4,408,484 _3787 Position (kb) 4406 4407Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4405.665 kb on + strand, within Pf6N2E2_3782at 4405.665 kb on + strand, within Pf6N2E2_3782at 4405.665 kb on + strand, within Pf6N2E2_3782at 4405.666 kb on - strand, within Pf6N2E2_3782at 4405.666 kb on - strand, within Pf6N2E2_3782at 4405.666 kb on - strand, within Pf6N2E2_3782at 4405.666 kb on - strand, within Pf6N2E2_3782at 4405.666 kb on - strand, within Pf6N2E2_3782at 4405.828 kb on - strand, within Pf6N2E2_3782at 4405.893 kb on + strandat 4405.893 kb on + strandat 4405.894 kb on - strandat 4405.894 kb on - strandat 4405.904 kb on + strandat 4405.904 kb on + strandat 4405.904 kb on + strandat 4405.905 kb on - strandat 4405.905 kb on - strandat 4405.905 kb on - strandat 4405.905 kb on - strandat 4405.905 kb on - strandat 4405.905 kb on - strandat 4405.905 kb on - strandat 4405.978 kb on + strand, within Pf6N2E2_3783at 4405.979 kb on - strand, within Pf6N2E2_3783at 4405.979 kb on - strand, within Pf6N2E2_3783at 4406.101 kb on + strand, within Pf6N2E2_3783at 4406.101 kb on + strand, within Pf6N2E2_3783at 4406.101 kb on + strand, within Pf6N2E2_3783at 4406.101 kb on + strand, within Pf6N2E2_3783at 4406.101 kb on + strand, within Pf6N2E2_3783at 4406.102 kb on - strand, within Pf6N2E2_3783at 4406.271 kb on + strandat 4406.562 kb on + strandat 4406.562 kb on + strandat 4406.562 kb on + strandat 4406.764 kb on + strand, within Pf6N2E2_3785at 4406.764 kb on + strand, within Pf6N2E2_3785at 4406.764 kb on + strand, within Pf6N2E2_3785at 4406.764 kb on + strand, within Pf6N2E2_3785at 4406.764 kb on + strand, within Pf6N2E2_3785at 4406.764 kb on + strand, within Pf6N2E2_3785at 4406.765 kb on - strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.928 kb on + strand, within Pf6N2E2_3785at 4406.962 kb on + strandat 4406.962 kb on + strandat 4406.962 kb on + strandat 4407.292 kb on + strand, within Pf6N2E2_3786at 4407.370 kb on + strand, within Pf6N2E2_3786at 4407.370 kb on + strand, within Pf6N2E2_3786at 4407.373 kb on + strand, within Pf6N2E2_3786at 4407.454 kb on + strand, within Pf6N2E2_3786at 4407.454 kb on + strand, within Pf6N2E2_3786at 4407.556 kb on + strand, within Pf6N2E2_3786at 4407.577 kb on + strand, within Pf6N2E2_3786at 4407.577 kb on + strand, within Pf6N2E2_3786at 4407.577 kb on + strand, within Pf6N2E2_3786

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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4,405,665 + Pf6N2E2_3782 0.37 -0.5
4,405,665 + Pf6N2E2_3782 0.37 +0.5
4,405,665 + Pf6N2E2_3782 0.37 +0.5
4,405,666 - Pf6N2E2_3782 0.37 -5.0
4,405,666 - Pf6N2E2_3782 0.37 -4.4
4,405,666 - Pf6N2E2_3782 0.37 -3.7
4,405,666 - Pf6N2E2_3782 0.37 -4.0
4,405,666 - Pf6N2E2_3782 0.37 -4.2
4,405,828 - Pf6N2E2_3782 0.78 -4.0
4,405,893 + -0.5
4,405,893 + -1.0
4,405,894 - -3.5
4,405,894 - -5.0
4,405,904 + -1.1
4,405,904 + -0.5
4,405,904 + -0.4
4,405,905 - -4.6
4,405,905 - -3.5
4,405,905 - -4.8
4,405,905 - -2.7
4,405,905 - -0.5
4,405,905 - -3.4
4,405,905 - -3.7
4,405,978 + Pf6N2E2_3783 0.21 -0.3
4,405,979 - Pf6N2E2_3783 0.21 -2.1
4,405,979 - Pf6N2E2_3783 0.21 -4.1
4,406,101 + Pf6N2E2_3783 0.58 -0.5
4,406,101 + Pf6N2E2_3783 0.58 +0.5
4,406,101 + Pf6N2E2_3783 0.58 -0.4
4,406,101 + Pf6N2E2_3783 0.58 +0.3
4,406,101 + Pf6N2E2_3783 0.58 +0.5
4,406,102 - Pf6N2E2_3783 0.58 +0.5
4,406,271 + -3.4
4,406,562 + -1.8
4,406,562 + -2.7
4,406,562 + -2.2
4,406,764 + Pf6N2E2_3785 0.47 -3.3
4,406,764 + Pf6N2E2_3785 0.47 -2.9
4,406,764 + Pf6N2E2_3785 0.47 -5.1
4,406,764 + Pf6N2E2_3785 0.47 -4.5
4,406,764 + Pf6N2E2_3785 0.47 -4.7
4,406,764 + Pf6N2E2_3785 0.47 -5.6
4,406,765 - Pf6N2E2_3785 0.48 -2.5
4,406,928 + Pf6N2E2_3785 0.86 -2.5
4,406,928 + Pf6N2E2_3785 0.86 +0.5
4,406,928 + Pf6N2E2_3785 0.86 +0.5
4,406,928 + Pf6N2E2_3785 0.86 -2.3
4,406,928 + Pf6N2E2_3785 0.86 +0.5
4,406,928 + Pf6N2E2_3785 0.86 -1.5
4,406,928 + Pf6N2E2_3785 0.86 -1.1
4,406,962 + -1.1
4,406,962 + -0.3
4,406,962 + -0.5
4,407,292 + Pf6N2E2_3786 0.39 -5.6
4,407,370 + Pf6N2E2_3786 0.48 -4.8
4,407,370 + Pf6N2E2_3786 0.48 +0.5
4,407,373 + Pf6N2E2_3786 0.49 -4.3
4,407,454 + Pf6N2E2_3786 0.59 -4.3
4,407,454 + Pf6N2E2_3786 0.59 -3.2
4,407,556 + Pf6N2E2_3786 0.72 -4.1
4,407,577 + Pf6N2E2_3786 0.74 -2.5
4,407,577 + Pf6N2E2_3786 0.74 -2.5
4,407,577 + Pf6N2E2_3786 0.74 -3.9

Or see this region's nucleotide sequence