Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3644 and Pf6N2E2_3645 are separated by 435 nucleotides Pf6N2E2_3645 and Pf6N2E2_3646 are separated by 193 nucleotides
Pf6N2E2_3644 - Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17), at 4,243,510 to 4,245,399
_3644
Pf6N2E2_3645 - Type I secretion outer membrane protein, TolC precursor, at 4,245,835 to 4,247,274
_3645
Pf6N2E2_3646 - 3-deoxy-D-manno-octulosonic-acid transferase (EC 2.-.-.-), at 4,247,468 to 4,248,718
_3646
Position (kb)
4245
4246
4247
4248 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 4244.930 kb on + strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.931 kb on - strand, within Pf6N2E2_3644 at 4244.966 kb on + strand, within Pf6N2E2_3644 at 4244.966 kb on + strand, within Pf6N2E2_3644 at 4244.967 kb on - strand, within Pf6N2E2_3644 at 4245.200 kb on + strand, within Pf6N2E2_3644 at 4245.201 kb on - strand, within Pf6N2E2_3644 at 4245.656 kb on + strand at 4245.656 kb on + strand at 4245.656 kb on + strand at 4245.657 kb on - strand at 4245.657 kb on - strand at 4245.657 kb on - strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.746 kb on + strand at 4245.747 kb on - strand at 4245.747 kb on - strand at 4245.747 kb on - strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.748 kb on + strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.749 kb on - strand at 4245.778 kb on + strand at 4245.779 kb on - strand at 4245.779 kb on - strand at 4245.822 kb on + strand at 4245.822 kb on + strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.823 kb on - strand at 4245.940 kb on + strand at 4245.940 kb on + strand at 4245.940 kb on + strand at 4246.126 kb on + strand, within Pf6N2E2_3645 at 4246.408 kb on + strand, within Pf6N2E2_3645 at 4246.409 kb on - strand, within Pf6N2E2_3645 at 4246.409 kb on - strand, within Pf6N2E2_3645 at 4246.495 kb on + strand, within Pf6N2E2_3645 at 4246.495 kb on + strand, within Pf6N2E2_3645 at 4246.496 kb on - strand, within Pf6N2E2_3645 at 4246.573 kb on - strand, within Pf6N2E2_3645 at 4246.599 kb on - strand, within Pf6N2E2_3645 at 4246.612 kb on + strand, within Pf6N2E2_3645 at 4246.613 kb on - strand, within Pf6N2E2_3645 at 4246.613 kb on - strand, within Pf6N2E2_3645 at 4246.635 kb on + strand, within Pf6N2E2_3645 at 4246.636 kb on - strand, within Pf6N2E2_3645 at 4246.691 kb on - strand, within Pf6N2E2_3645 at 4246.691 kb on - strand, within Pf6N2E2_3645 at 4246.794 kb on + strand, within Pf6N2E2_3645 at 4247.065 kb on + strand, within Pf6N2E2_3645 at 4247.065 kb on + strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.066 kb on - strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.083 kb on + strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.084 kb on - strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.104 kb on + strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.105 kb on - strand, within Pf6N2E2_3645 at 4247.179 kb on + strand at 4247.180 kb on - strand at 4247.194 kb on + strand at 4247.194 kb on + strand at 4247.195 kb on - strand at 4247.195 kb on - strand at 4247.195 kb on - strand at 4247.272 kb on + strand at 4247.273 kb on - strand at 4247.277 kb on + strand at 4247.277 kb on + strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.278 kb on - strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.285 kb on + strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.286 kb on - strand at 4247.300 kb on - strand at 4247.348 kb on + strand at 4247.348 kb on + strand at 4247.348 kb on + strand at 4247.411 kb on + strand at 4247.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 4,244,930 + Pf6N2E2_3644 0.75 +0.5 4,244,931 - Pf6N2E2_3644 0.75 -0.4 4,244,931 - Pf6N2E2_3644 0.75 -1.0 4,244,931 - Pf6N2E2_3644 0.75 +0.5 4,244,931 - Pf6N2E2_3644 0.75 -0.2 4,244,966 + Pf6N2E2_3644 0.77 -0.5 4,244,966 + Pf6N2E2_3644 0.77 +0.5 4,244,967 - Pf6N2E2_3644 0.77 -0.5 4,245,200 + Pf6N2E2_3644 0.89 +0.5 4,245,201 - Pf6N2E2_3644 0.89 -1.1 4,245,656 + +0.5 4,245,656 + -0.5 4,245,656 + -0.5 4,245,657 - -0.6 4,245,657 - -1.8 4,245,657 - -0.5 4,245,746 + +0.5 4,245,746 + -2.4 4,245,746 + +0.5 4,245,746 + -0.5 4,245,746 + -0.5 4,245,746 + -0.5 4,245,746 + -0.5 4,245,747 - -1.1 4,245,747 - -0.5 4,245,747 - -1.1 4,245,748 + -0.6 4,245,748 + -0.8 4,245,748 + +0.1 4,245,748 + -0.5 4,245,748 + -0.1 4,245,749 - -0.5 4,245,749 - +0.5 4,245,749 - -1.1 4,245,749 - -1.1 4,245,749 - +0.5 4,245,778 + -0.5 4,245,779 - +0.5 4,245,779 - -1.8 4,245,822 + +0.5 4,245,822 + +0.5 4,245,823 - +0.5 4,245,823 - -1.1 4,245,823 - +0.5 4,245,823 - -4.5 4,245,823 - +0.5 4,245,823 - -1.1 4,245,823 - -6.0 4,245,823 - -0.5 4,245,940 + -1.1 4,245,940 + +0.5 4,245,940 + -1.1 4,246,126 + Pf6N2E2_3645 0.20 +0.5 4,246,408 + Pf6N2E2_3645 0.40 -1.1 4,246,409 - Pf6N2E2_3645 0.40 -0.5 4,246,409 - Pf6N2E2_3645 0.40 -1.8 4,246,495 + Pf6N2E2_3645 0.46 -1.5 4,246,495 + Pf6N2E2_3645 0.46 +0.5 4,246,496 - Pf6N2E2_3645 0.46 -1.1 4,246,573 - Pf6N2E2_3645 0.51 -0.5 4,246,599 - Pf6N2E2_3645 0.53 -1.1 4,246,612 + Pf6N2E2_3645 0.54 +0.5 4,246,613 - Pf6N2E2_3645 0.54 +0.5 4,246,613 - Pf6N2E2_3645 0.54 -5.8 4,246,635 + Pf6N2E2_3645 0.56 -4.2 4,246,636 - Pf6N2E2_3645 0.56 +0.5 4,246,691 - Pf6N2E2_3645 0.59 -1.8 4,246,691 - Pf6N2E2_3645 0.59 -3.4 4,246,794 + Pf6N2E2_3645 0.67 +0.5 4,247,065 + Pf6N2E2_3645 0.85 -1.5 4,247,065 + Pf6N2E2_3645 0.85 -0.5 4,247,066 - Pf6N2E2_3645 0.85 -0.5 4,247,066 - Pf6N2E2_3645 0.85 -0.1 4,247,066 - Pf6N2E2_3645 0.85 -4.2 4,247,066 - Pf6N2E2_3645 0.85 +0.5 4,247,066 - Pf6N2E2_3645 0.85 -1.1 4,247,083 + Pf6N2E2_3645 0.87 -2.5 4,247,083 + Pf6N2E2_3645 0.87 -4.6 4,247,083 + Pf6N2E2_3645 0.87 -0.5 4,247,083 + Pf6N2E2_3645 0.87 -1.1 4,247,084 - Pf6N2E2_3645 0.87 -5.7 4,247,084 - Pf6N2E2_3645 0.87 +0.5 4,247,084 - Pf6N2E2_3645 0.87 +0.5 4,247,104 + Pf6N2E2_3645 0.88 +0.5 4,247,104 + Pf6N2E2_3645 0.88 -0.5 4,247,104 + Pf6N2E2_3645 0.88 -1.1 4,247,104 + Pf6N2E2_3645 0.88 -1.1 4,247,104 + Pf6N2E2_3645 0.88 -1.1 4,247,105 - Pf6N2E2_3645 0.88 +0.5 4,247,105 - Pf6N2E2_3645 0.88 -0.5 4,247,105 - Pf6N2E2_3645 0.88 -1.1 4,247,105 - Pf6N2E2_3645 0.88 -1.5 4,247,105 - Pf6N2E2_3645 0.88 -4.7 4,247,105 - Pf6N2E2_3645 0.88 -5.7 4,247,105 - Pf6N2E2_3645 0.88 -2.2 4,247,179 + -1.1 4,247,180 - +0.5 4,247,194 + +0.5 4,247,194 + -5.2 4,247,195 - -0.5 4,247,195 - +0.5 4,247,195 - +0.5 4,247,272 + -0.5 4,247,273 - -0.8 4,247,277 + +1.5 4,247,277 + +0.5 4,247,278 - -0.8 4,247,278 - +0.5 4,247,278 - -0.5 4,247,278 - -0.5 4,247,285 + +0.2 4,247,285 + -0.5 4,247,285 + -0.5 4,247,285 + -0.7 4,247,285 + -1.9 4,247,285 + +0.5 4,247,285 + -0.5 4,247,285 + -1.1 4,247,285 + +0.5 4,247,286 - -0.5 4,247,286 - +0.5 4,247,286 - -0.5 4,247,286 - -1.1 4,247,286 - -0.8 4,247,286 - +0.5 4,247,286 - -2.1 4,247,300 - +0.5 4,247,348 + -1.1 4,247,348 + -0.5 4,247,348 + -0.5 4,247,411 + +0.5 4,247,412 - +0.5
Or see this region's nucleotide sequence