Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3620 and Pf6N2E2_3621 overlap by 19 nucleotides Pf6N2E2_3621 and Pf6N2E2_3622 are separated by 264 nucleotides Pf6N2E2_3622 and Pf6N2E2_3623 are separated by 109 nucleotides
Pf6N2E2_3620 - Epoxyqueuosine (oQ) reductase QueG, at 4,215,840 to 4,216,919
_3620
Pf6N2E2_3621 - putative transporter, required for glycine utilization (from data) , at 4,216,901 to 4,217,512
_3621
Pf6N2E2_3622 - 3'-to-5' oligoribonuclease (orn), at 4,217,777 to 4,218,319
_3622
Pf6N2E2_3623 - Ribosome small subunit-stimulated GTPase EngC, at 4,218,429 to 4,219,460
_3623
Position (kb)
4216
4217
4218 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.002 kb on + strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.003 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.087 kb on - strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.140 kb on + strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.141 kb on - strand, within Pf6N2E2_3620 at 4216.152 kb on + strand, within Pf6N2E2_3620 at 4216.170 kb on + strand, within Pf6N2E2_3620 at 4216.317 kb on - strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.353 kb on + strand, within Pf6N2E2_3620 at 4216.354 kb on - strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.457 kb on + strand, within Pf6N2E2_3620 at 4216.458 kb on - strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.479 kb on + strand, within Pf6N2E2_3620 at 4216.480 kb on - strand, within Pf6N2E2_3620 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4216.988 kb on - strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.037 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.070 kb on + strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.071 kb on - strand, within Pf6N2E2_3621 at 4217.099 kb on - strand, within Pf6N2E2_3621 at 4217.099 kb on - strand, within Pf6N2E2_3621 at 4217.321 kb on + strand, within Pf6N2E2_3621 at 4217.321 kb on + strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.322 kb on - strand, within Pf6N2E2_3621 at 4217.326 kb on - strand, within Pf6N2E2_3621 at 4217.326 kb on - strand, within Pf6N2E2_3621 at 4217.496 kb on + strand at 4217.496 kb on + strand at 4217.496 kb on + strand at 4217.497 kb on - strand at 4217.598 kb on + strand at 4217.598 kb on + strand at 4217.598 kb on + strand at 4217.599 kb on - strand at 4217.599 kb on - strand at 4218.338 kb on - strand at 4218.338 kb on - strand at 4218.350 kb on + strand at 4218.351 kb on - strand at 4218.351 kb on - strand at 4218.355 kb on + strand at 4218.356 kb on - strand at 4218.356 kb on - strand at 4218.394 kb on - strand at 4218.398 kb on + strand at 4218.398 kb on + strand at 4218.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 4,216,002 + Pf6N2E2_3620 0.15 -0.5 4,216,002 + Pf6N2E2_3620 0.15 +0.5 4,216,002 + Pf6N2E2_3620 0.15 +0.5 4,216,003 - Pf6N2E2_3620 0.15 +0.5 4,216,003 - Pf6N2E2_3620 0.15 +0.5 4,216,003 - Pf6N2E2_3620 0.15 -1.5 4,216,003 - Pf6N2E2_3620 0.15 -0.5 4,216,087 - Pf6N2E2_3620 0.23 +0.5 4,216,087 - Pf6N2E2_3620 0.23 +1.5 4,216,087 - Pf6N2E2_3620 0.23 -0.5 4,216,140 + Pf6N2E2_3620 0.28 +0.5 4,216,140 + Pf6N2E2_3620 0.28 +0.5 4,216,140 + Pf6N2E2_3620 0.28 -0.5 4,216,141 - Pf6N2E2_3620 0.28 -2.1 4,216,141 - Pf6N2E2_3620 0.28 +0.5 4,216,141 - Pf6N2E2_3620 0.28 -0.5 4,216,152 + Pf6N2E2_3620 0.29 -1.1 4,216,170 + Pf6N2E2_3620 0.31 -0.5 4,216,317 - Pf6N2E2_3620 0.44 -1.2 4,216,353 + Pf6N2E2_3620 0.47 -1.1 4,216,353 + Pf6N2E2_3620 0.47 +0.5 4,216,353 + Pf6N2E2_3620 0.47 +0.5 4,216,354 - Pf6N2E2_3620 0.48 +0.5 4,216,457 + Pf6N2E2_3620 0.57 -0.5 4,216,457 + Pf6N2E2_3620 0.57 -0.5 4,216,457 + Pf6N2E2_3620 0.57 +0.5 4,216,458 - Pf6N2E2_3620 0.57 +0.5 4,216,479 + Pf6N2E2_3620 0.59 -1.1 4,216,479 + Pf6N2E2_3620 0.59 -0.5 4,216,480 - Pf6N2E2_3620 0.59 -1.1 4,216,988 - Pf6N2E2_3621 0.14 -0.8 4,216,988 - Pf6N2E2_3621 0.14 -1.1 4,216,988 - Pf6N2E2_3621 0.14 +0.5 4,217,037 + Pf6N2E2_3621 0.22 -0.5 4,217,037 + Pf6N2E2_3621 0.22 +0.5 4,217,037 + Pf6N2E2_3621 0.22 +0.5 4,217,037 + Pf6N2E2_3621 0.22 +0.5 4,217,037 + Pf6N2E2_3621 0.22 +0.5 4,217,037 + Pf6N2E2_3621 0.22 -2.7 4,217,037 + Pf6N2E2_3621 0.22 -1.5 4,217,070 + Pf6N2E2_3621 0.28 +0.5 4,217,070 + Pf6N2E2_3621 0.28 +0.5 4,217,070 + Pf6N2E2_3621 0.28 -0.5 4,217,071 - Pf6N2E2_3621 0.28 -0.8 4,217,071 - Pf6N2E2_3621 0.28 +0.5 4,217,071 - Pf6N2E2_3621 0.28 +0.5 4,217,099 - Pf6N2E2_3621 0.32 -0.5 4,217,099 - Pf6N2E2_3621 0.32 -3.8 4,217,321 + Pf6N2E2_3621 0.69 +0.5 4,217,321 + Pf6N2E2_3621 0.69 +0.5 4,217,322 - Pf6N2E2_3621 0.69 -3.0 4,217,322 - Pf6N2E2_3621 0.69 -4.8 4,217,322 - Pf6N2E2_3621 0.69 -0.5 4,217,322 - Pf6N2E2_3621 0.69 +0.5 4,217,326 - Pf6N2E2_3621 0.69 -4.0 4,217,326 - Pf6N2E2_3621 0.69 -3.6 4,217,496 + +0.5 4,217,496 + -2.9 4,217,496 + -3.3 4,217,497 - -0.5 4,217,598 + -0.3 4,217,598 + -2.5 4,217,598 + -0.5 4,217,599 - -0.5 4,217,599 - -1.1 4,218,338 - +0.5 4,218,338 - -0.5 4,218,350 + +0.5 4,218,351 - +0.1 4,218,351 - -0.5 4,218,355 + +0.5 4,218,356 - +0.5 4,218,356 - -0.8 4,218,394 - +0.5 4,218,398 + -2.3 4,218,398 + -0.0 4,218,417 + +0.5
Or see this region's nucleotide sequence