Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3348 and Pf6N2E2_6117 are separated by 13 nucleotides Pf6N2E2_6117 and Pf6N2E2_3349 are separated by 125 nucleotides Pf6N2E2_3349 and Pf6N2E2_3350 are separated by 194 nucleotides Pf6N2E2_3350 and Pf6N2E2_3351 are separated by 143 nucleotides
Pf6N2E2_3348 - hypothetical protein, at 3,876,297 to 3,876,845
_3348
Pf6N2E2_6117 - tRNA-Thr-CGT, at 3,876,859 to 3,876,931
_6117
Pf6N2E2_3349 - Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8), at 3,877,057 to 3,879,162
_3349
Pf6N2E2_3350 - SSU ribosomal protein S15p (S13e), at 3,879,357 to 3,879,626
_3350
Pf6N2E2_3351 - tRNA pseudouridine synthase B (EC 4.2.1.70), at 3,879,770 to 3,880,687
_3351
Position (kb)
3877
3878
3879
3880 Strain fitness (log2 ratio)
-2
-1
0
1 at 3876.077 kb on + strand at 3876.078 kb on - strand at 3876.145 kb on + strand at 3876.145 kb on + strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.146 kb on - strand at 3876.147 kb on + strand at 3876.147 kb on + strand at 3876.148 kb on - strand at 3876.148 kb on - strand at 3876.169 kb on - strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.180 kb on + strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.181 kb on - strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.186 kb on + strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.187 kb on - strand at 3876.209 kb on - strand at 3876.417 kb on + strand, within Pf6N2E2_3348 at 3876.417 kb on + strand, within Pf6N2E2_3348 at 3876.429 kb on - strand, within Pf6N2E2_3348 at 3876.445 kb on - strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.462 kb on + strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.463 kb on - strand, within Pf6N2E2_3348 at 3876.844 kb on - strand at 3876.844 kb on - strand at 3876.923 kb on + strand, within Pf6N2E2_6117 at 3876.924 kb on - strand at 3876.944 kb on + strand at 3876.944 kb on + strand at 3876.944 kb on + strand at 3876.945 kb on - strand at 3876.945 kb on - strand at 3876.996 kb on + strand at 3876.996 kb on + strand at 3876.997 kb on - strand at 3876.997 kb on - strand at 3876.997 kb on - strand at 3877.286 kb on + strand, within Pf6N2E2_3349 at 3877.286 kb on + strand, within Pf6N2E2_3349 at 3877.286 kb on + strand, within Pf6N2E2_3349 at 3877.287 kb on - strand, within Pf6N2E2_3349 at 3877.287 kb on - strand, within Pf6N2E2_3349 at 3879.272 kb on - strand at 3879.272 kb on - strand at 3879.848 kb on - strand at 3879.897 kb on - strand, within Pf6N2E2_3351 at 3879.919 kb on - strand, within Pf6N2E2_3351 at 3880.102 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351 at 3880.153 kb on - strand, within Pf6N2E2_3351
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,876,077 + -1.2 3,876,078 - -0.6 3,876,145 + +0.4 3,876,145 + -0.8 3,876,146 - -0.6 3,876,146 - -0.5 3,876,146 - -0.6 3,876,146 - -0.6 3,876,146 - -0.6 3,876,146 - +0.4 3,876,147 + -1.0 3,876,147 + +0.4 3,876,148 - -1.2 3,876,148 - -1.2 3,876,169 - -0.6 3,876,180 + +0.4 3,876,180 + +0.4 3,876,180 + -0.6 3,876,180 + +0.4 3,876,180 + -0.2 3,876,180 + -0.6 3,876,180 + -0.7 3,876,180 + -0.6 3,876,180 + -0.9 3,876,181 - +0.4 3,876,181 - -0.7 3,876,181 - +0.4 3,876,181 - +0.6 3,876,181 - -0.3 3,876,181 - -1.6 3,876,186 + +0.4 3,876,186 + +0.4 3,876,186 + -0.2 3,876,186 + -0.4 3,876,187 - -0.5 3,876,187 - +0.4 3,876,187 - -1.2 3,876,187 - +0.4 3,876,209 - -0.6 3,876,417 + Pf6N2E2_3348 0.22 +0.4 3,876,417 + Pf6N2E2_3348 0.22 +0.4 3,876,429 - Pf6N2E2_3348 0.24 +0.4 3,876,445 - Pf6N2E2_3348 0.27 -1.2 3,876,462 + Pf6N2E2_3348 0.30 -0.6 3,876,462 + Pf6N2E2_3348 0.30 +0.4 3,876,462 + Pf6N2E2_3348 0.30 -0.6 3,876,462 + Pf6N2E2_3348 0.30 +0.4 3,876,462 + Pf6N2E2_3348 0.30 -0.3 3,876,462 + Pf6N2E2_3348 0.30 +0.4 3,876,463 - Pf6N2E2_3348 0.30 +0.4 3,876,463 - Pf6N2E2_3348 0.30 -1.6 3,876,463 - Pf6N2E2_3348 0.30 -0.6 3,876,463 - Pf6N2E2_3348 0.30 -1.2 3,876,463 - Pf6N2E2_3348 0.30 +0.4 3,876,844 - -0.6 3,876,844 - -0.6 3,876,923 + Pf6N2E2_6117 0.88 -0.5 3,876,924 - +0.4 3,876,944 + -1.2 3,876,944 + +0.4 3,876,944 + -1.2 3,876,945 - -0.6 3,876,945 - -0.6 3,876,996 + -1.1 3,876,996 + -0.6 3,876,997 - -1.2 3,876,997 - -0.6 3,876,997 - +0.4 3,877,286 + Pf6N2E2_3349 0.11 -1.2 3,877,286 + Pf6N2E2_3349 0.11 +0.4 3,877,286 + Pf6N2E2_3349 0.11 -1.2 3,877,287 - Pf6N2E2_3349 0.11 +0.4 3,877,287 - Pf6N2E2_3349 0.11 -1.9 3,879,272 - +0.4 3,879,272 - -0.6 3,879,848 - -2.6 3,879,897 - Pf6N2E2_3351 0.14 -0.6 3,879,919 - Pf6N2E2_3351 0.16 -0.6 3,880,102 - Pf6N2E2_3351 0.36 +0.4 3,880,153 - Pf6N2E2_3351 0.42 +0.4 3,880,153 - Pf6N2E2_3351 0.42 +0.4 3,880,153 - Pf6N2E2_3351 0.42 +0.4 3,880,153 - Pf6N2E2_3351 0.42 +0.4
Or see this region's nucleotide sequence