Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3262 and Pf6N2E2_3263 are separated by 0 nucleotides Pf6N2E2_3263 and Pf6N2E2_3264 are separated by 7 nucleotides Pf6N2E2_3264 and Pf6N2E2_3265 overlap by 14 nucleotides Pf6N2E2_3265 and Pf6N2E2_3266 overlap by 1 nucleotides
Pf6N2E2_3262 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 3,769,774 to 3,770,748
_3262
Pf6N2E2_3263 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase (EC 3.1.3.45), at 3,770,749 to 3,771,273
_3263
Pf6N2E2_3264 - Uncharacterized protein YrbK clustered with lipopolysaccharide transporters, at 3,771,281 to 3,771,850
_3264
Pf6N2E2_3265 - LptA, protein essential for LPS transport across the periplasm, at 3,771,837 to 3,772,388
_3265
Pf6N2E2_3266 - Lipopolysaccharide ABC transporter, ATP-binding protein LptB, at 3,772,388 to 3,773,113
_3266
Position (kb) 3771
3772 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3770.746 kb on + strand at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.716 kb on + strand, within Pf6N2E2_3264 at 3771.825 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,770,746 + -3.4 3,771,716 + Pf6N2E2_3264 0.76 -3.4 3,771,716 + Pf6N2E2_3264 0.76 -2.7 3,771,716 + Pf6N2E2_3264 0.76 -3.2 3,771,716 + Pf6N2E2_3264 0.76 -2.6 3,771,825 + +0.4
Or see this region's nucleotide sequence