Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3233 and Pf6N2E2_3234 are separated by 71 nucleotides Pf6N2E2_3234 and Pf6N2E2_3235 are separated by 21 nucleotides Pf6N2E2_3235 and Pf6N2E2_3236 are separated by 327 nucleotides Pf6N2E2_3236 and Pf6N2E2_3237 are separated by 24 nucleotides
Pf6N2E2_3233 - Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2), at 3,737,579 to 3,739,201
_3233
Pf6N2E2_3234 - mandelate racemase/muconate lactonizing enzyme family protein, at 3,739,273 to 3,740,334
_3234
Pf6N2E2_3235 - hypothetical protein, at 3,740,356 to 3,740,493
_3235
Pf6N2E2_3236 - hypothetical protein, at 3,740,821 to 3,740,934
_3236
Pf6N2E2_3237 - hypothetical protein, at 3,740,959 to 3,741,186
_3237
Position (kb)
3739
3740
3741 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3738.318 kb on + strand, within Pf6N2E2_3233 at 3738.318 kb on + strand, within Pf6N2E2_3233 at 3738.318 kb on + strand, within Pf6N2E2_3233 at 3738.318 kb on + strand, within Pf6N2E2_3233 at 3738.318 kb on + strand, within Pf6N2E2_3233 at 3738.318 kb on + strand, within Pf6N2E2_3233 at 3738.319 kb on - strand, within Pf6N2E2_3233 at 3738.319 kb on - strand, within Pf6N2E2_3233 at 3738.321 kb on + strand, within Pf6N2E2_3233 at 3738.321 kb on + strand, within Pf6N2E2_3233 at 3738.322 kb on - strand, within Pf6N2E2_3233 at 3738.504 kb on + strand, within Pf6N2E2_3233 at 3738.505 kb on - strand, within Pf6N2E2_3233 at 3738.505 kb on - strand, within Pf6N2E2_3233 at 3739.003 kb on - strand, within Pf6N2E2_3233 at 3739.003 kb on - strand, within Pf6N2E2_3233 at 3739.146 kb on + strand at 3739.217 kb on + strand at 3739.217 kb on + strand at 3739.217 kb on + strand at 3739.217 kb on + strand at 3739.218 kb on - strand at 3739.218 kb on - strand at 3739.219 kb on + strand at 3739.219 kb on + strand at 3739.220 kb on - strand at 3739.220 kb on - strand at 3739.220 kb on - strand at 3739.220 kb on - strand at 3739.220 kb on - strand at 3739.223 kb on + strand at 3739.223 kb on + strand at 3739.223 kb on + strand at 3739.223 kb on + strand at 3739.223 kb on + strand at 3739.223 kb on + strand at 3739.225 kb on + strand at 3739.303 kb on - strand at 3739.305 kb on - strand at 3739.305 kb on - strand at 3739.305 kb on - strand at 3739.351 kb on + strand at 3739.352 kb on - strand at 3739.412 kb on + strand, within Pf6N2E2_3234 at 3739.412 kb on + strand, within Pf6N2E2_3234 at 3739.413 kb on - strand, within Pf6N2E2_3234 at 3739.445 kb on + strand, within Pf6N2E2_3234 at 3739.700 kb on + strand, within Pf6N2E2_3234 at 3739.700 kb on + strand, within Pf6N2E2_3234 at 3739.701 kb on - strand, within Pf6N2E2_3234 at 3739.701 kb on - strand, within Pf6N2E2_3234 at 3739.797 kb on - strand, within Pf6N2E2_3234 at 3740.282 kb on + strand at 3740.283 kb on - strand at 3740.628 kb on + strand at 3740.629 kb on - strand at 3740.659 kb on + strand at 3740.666 kb on + strand at 3740.667 kb on - strand at 3740.718 kb on + strand at 3740.788 kb on - strand at 3740.961 kb on - strand at 3741.055 kb on - strand, within Pf6N2E2_3237 at 3741.080 kb on + strand, within Pf6N2E2_3237 at 3741.080 kb on + strand, within Pf6N2E2_3237 at 3741.096 kb on - strand, within Pf6N2E2_3237 at 3741.186 kb on + strand at 3741.186 kb on + strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.187 kb on - strand at 3741.204 kb on + strand at 3741.204 kb on + strand at 3741.204 kb on + strand at 3741.204 kb on + strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.205 kb on - strand at 3741.261 kb on + strand at 3741.261 kb on + strand at 3741.261 kb on + strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.262 kb on - strand at 3741.271 kb on + strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand at 3741.272 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,738,318 + Pf6N2E2_3233 0.46 -0.5 3,738,318 + Pf6N2E2_3233 0.46 -1.6 3,738,318 + Pf6N2E2_3233 0.46 -1.2 3,738,318 + Pf6N2E2_3233 0.46 +0.4 3,738,318 + Pf6N2E2_3233 0.46 +0.4 3,738,318 + Pf6N2E2_3233 0.46 +0.4 3,738,319 - Pf6N2E2_3233 0.46 +0.4 3,738,319 - Pf6N2E2_3233 0.46 -0.6 3,738,321 + Pf6N2E2_3233 0.46 +0.4 3,738,321 + Pf6N2E2_3233 0.46 -0.7 3,738,322 - Pf6N2E2_3233 0.46 +0.1 3,738,504 + Pf6N2E2_3233 0.57 +0.4 3,738,505 - Pf6N2E2_3233 0.57 -1.9 3,738,505 - Pf6N2E2_3233 0.57 -1.0 3,739,003 - Pf6N2E2_3233 0.88 +0.4 3,739,003 - Pf6N2E2_3233 0.88 +0.4 3,739,146 + -1.6 3,739,217 + +0.4 3,739,217 + +0.4 3,739,217 + -0.9 3,739,217 + -0.6 3,739,218 - -1.6 3,739,218 - -0.8 3,739,219 + -1.6 3,739,219 + -3.7 3,739,220 - -1.9 3,739,220 - -0.8 3,739,220 - -0.3 3,739,220 - +0.4 3,739,220 - -0.6 3,739,223 + -1.6 3,739,223 + -0.4 3,739,223 + -0.6 3,739,223 + +0.4 3,739,223 + -0.6 3,739,223 + -0.6 3,739,225 + +0.4 3,739,303 - +0.4 3,739,305 - +0.4 3,739,305 - +0.4 3,739,305 - -0.5 3,739,351 + +0.4 3,739,352 - -0.6 3,739,412 + Pf6N2E2_3234 0.13 -0.6 3,739,412 + Pf6N2E2_3234 0.13 -0.2 3,739,413 - Pf6N2E2_3234 0.13 -2.2 3,739,445 + Pf6N2E2_3234 0.16 -0.6 3,739,700 + Pf6N2E2_3234 0.40 -1.2 3,739,700 + Pf6N2E2_3234 0.40 -1.6 3,739,701 - Pf6N2E2_3234 0.40 +0.4 3,739,701 - Pf6N2E2_3234 0.40 +0.4 3,739,797 - Pf6N2E2_3234 0.49 +0.4 3,740,282 + -0.6 3,740,283 - -0.5 3,740,628 + +0.4 3,740,629 - +0.4 3,740,659 + +0.4 3,740,666 + -0.6 3,740,667 - -0.7 3,740,718 + -0.6 3,740,788 - -1.2 3,740,961 - -1.2 3,741,055 - Pf6N2E2_3237 0.42 +0.4 3,741,080 + Pf6N2E2_3237 0.53 -0.9 3,741,080 + Pf6N2E2_3237 0.53 +0.4 3,741,096 - Pf6N2E2_3237 0.60 +0.4 3,741,186 + -0.9 3,741,186 + -0.3 3,741,187 - -0.7 3,741,187 - +0.4 3,741,187 - -1.2 3,741,187 - +0.4 3,741,187 - +0.4 3,741,187 - -1.2 3,741,204 + -0.0 3,741,204 + -0.6 3,741,204 + +0.4 3,741,204 + -0.6 3,741,205 - -0.6 3,741,205 - -0.5 3,741,205 - -1.9 3,741,205 - -0.9 3,741,205 - +0.4 3,741,205 - -1.6 3,741,205 - -0.6 3,741,205 - +0.4 3,741,261 + -0.6 3,741,261 + +0.4 3,741,261 + +0.4 3,741,262 - +0.4 3,741,262 - +0.4 3,741,262 - +0.4 3,741,262 - -1.2 3,741,262 - -0.6 3,741,262 - +0.4 3,741,262 - -0.6 3,741,271 + -0.3 3,741,272 - +0.4 3,741,272 - +0.4 3,741,272 - -2.2 3,741,272 - +0.4 3,741,272 - -0.4 3,741,272 - -0.6 3,741,272 - -0.6 3,741,272 - -0.6
Or see this region's nucleotide sequence