Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3170 and Pf6N2E2_3171 are separated by 194 nucleotides Pf6N2E2_3171 and Pf6N2E2_3172 are separated by 63 nucleotides Pf6N2E2_3172 and Pf6N2E2_3173 overlap by 13 nucleotides
Pf6N2E2_3170 - 2-isopropylmalate synthase (EC 2.3.3.13), at 3,665,153 to 3,666,832
_3170
Pf6N2E2_3171 - hypothetical protein, at 3,667,027 to 3,667,440
_3171
Pf6N2E2_3172 - Aliphatic amidase AmiE (EC 3.5.1.4), at 3,667,504 to 3,668,298
_3172
Pf6N2E2_3173 - Aspartate aminotransferase (EC 2.6.1.1), at 3,668,286 to 3,669,434
_3173
Position (kb)
3667
3668 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3666.029 kb on + strand, within Pf6N2E2_3170 at 3666.131 kb on + strand, within Pf6N2E2_3170 at 3666.132 kb on - strand, within Pf6N2E2_3170 at 3666.132 kb on - strand, within Pf6N2E2_3170 at 3666.132 kb on - strand, within Pf6N2E2_3170 at 3666.233 kb on + strand, within Pf6N2E2_3170 at 3666.233 kb on + strand, within Pf6N2E2_3170 at 3666.233 kb on + strand, within Pf6N2E2_3170 at 3666.234 kb on - strand, within Pf6N2E2_3170 at 3666.234 kb on - strand, within Pf6N2E2_3170 at 3666.234 kb on - strand, within Pf6N2E2_3170 at 3666.269 kb on + strand, within Pf6N2E2_3170 at 3666.269 kb on + strand, within Pf6N2E2_3170 at 3666.269 kb on + strand, within Pf6N2E2_3170 at 3666.270 kb on - strand, within Pf6N2E2_3170 at 3666.270 kb on - strand, within Pf6N2E2_3170 at 3666.339 kb on - strand, within Pf6N2E2_3170 at 3666.387 kb on + strand, within Pf6N2E2_3170 at 3666.387 kb on + strand, within Pf6N2E2_3170 at 3666.387 kb on + strand at 3666.387 kb on + strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.388 kb on - strand, within Pf6N2E2_3170 at 3666.458 kb on + strand, within Pf6N2E2_3170 at 3666.459 kb on - strand, within Pf6N2E2_3170 at 3666.529 kb on - strand, within Pf6N2E2_3170 at 3666.647 kb on + strand, within Pf6N2E2_3170 at 3666.647 kb on + strand, within Pf6N2E2_3170 at 3666.647 kb on + strand, within Pf6N2E2_3170 at 3666.648 kb on - strand, within Pf6N2E2_3170 at 3666.648 kb on - strand, within Pf6N2E2_3170 at 3666.695 kb on + strand at 3666.835 kb on + strand at 3666.835 kb on + strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.836 kb on - strand at 3666.912 kb on + strand at 3666.912 kb on + strand at 3666.912 kb on + strand at 3666.913 kb on - strand at 3667.389 kb on + strand, within Pf6N2E2_3171 at 3667.414 kb on + strand at 3667.414 kb on + strand at 3667.431 kb on + strand at 3667.432 kb on - strand at 3667.440 kb on + strand at 3667.559 kb on + strand at 3667.667 kb on + strand, within Pf6N2E2_3172 at 3667.667 kb on + strand, within Pf6N2E2_3172 at 3667.668 kb on - strand, within Pf6N2E2_3172 at 3667.715 kb on + strand, within Pf6N2E2_3172 at 3667.716 kb on - strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.793 kb on + strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.794 kb on - strand, within Pf6N2E2_3172 at 3667.795 kb on + strand, within Pf6N2E2_3172 at 3667.795 kb on + strand, within Pf6N2E2_3172 at 3667.796 kb on - strand, within Pf6N2E2_3172 at 3667.796 kb on - strand, within Pf6N2E2_3172 at 3667.851 kb on - strand, within Pf6N2E2_3172 at 3667.916 kb on + strand, within Pf6N2E2_3172 at 3667.917 kb on - strand, within Pf6N2E2_3172 at 3667.961 kb on + strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3667.962 kb on - strand, within Pf6N2E2_3172 at 3668.298 kb on + strand at 3668.298 kb on + strand at 3668.299 kb on - strand at 3668.299 kb on - strand at 3668.299 kb on - strand at 3668.383 kb on + strand at 3668.384 kb on - strand at 3668.384 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,666,029 + Pf6N2E2_3170 0.52 +0.4 3,666,131 + Pf6N2E2_3170 0.58 -0.6 3,666,132 - Pf6N2E2_3170 0.58 -0.8 3,666,132 - Pf6N2E2_3170 0.58 -0.5 3,666,132 - Pf6N2E2_3170 0.58 -1.2 3,666,233 + Pf6N2E2_3170 0.64 -0.6 3,666,233 + Pf6N2E2_3170 0.64 +0.4 3,666,233 + Pf6N2E2_3170 0.64 -0.6 3,666,234 - Pf6N2E2_3170 0.64 -1.2 3,666,234 - Pf6N2E2_3170 0.64 +0.4 3,666,234 - Pf6N2E2_3170 0.64 -1.2 3,666,269 + Pf6N2E2_3170 0.66 +0.4 3,666,269 + Pf6N2E2_3170 0.66 +0.4 3,666,269 + Pf6N2E2_3170 0.66 -1.4 3,666,270 - Pf6N2E2_3170 0.66 -0.6 3,666,270 - Pf6N2E2_3170 0.66 -0.6 3,666,339 - Pf6N2E2_3170 0.71 -0.6 3,666,387 + Pf6N2E2_3170 0.73 -1.0 3,666,387 + Pf6N2E2_3170 0.73 +0.4 3,666,387 + +0.4 3,666,387 + Pf6N2E2_3170 0.73 -0.5 3,666,388 - Pf6N2E2_3170 0.74 +0.4 3,666,388 - Pf6N2E2_3170 0.74 -0.4 3,666,388 - Pf6N2E2_3170 0.74 -0.6 3,666,388 - Pf6N2E2_3170 0.74 -1.0 3,666,458 + Pf6N2E2_3170 0.78 +0.4 3,666,459 - Pf6N2E2_3170 0.78 -1.2 3,666,529 - Pf6N2E2_3170 0.82 +0.4 3,666,647 + Pf6N2E2_3170 0.89 -1.6 3,666,647 + Pf6N2E2_3170 0.89 -1.2 3,666,647 + Pf6N2E2_3170 0.89 -0.7 3,666,648 - Pf6N2E2_3170 0.89 -2.9 3,666,648 - Pf6N2E2_3170 0.89 -0.6 3,666,695 + +0.4 3,666,835 + -0.5 3,666,835 + -0.5 3,666,836 - -0.6 3,666,836 - -1.6 3,666,836 - -0.2 3,666,836 - +0.4 3,666,836 - -1.2 3,666,912 + +0.4 3,666,912 + -0.6 3,666,912 + +0.4 3,666,913 - -0.9 3,667,389 + Pf6N2E2_3171 0.87 -2.2 3,667,414 + +0.4 3,667,414 + -2.6 3,667,431 + +0.4 3,667,432 - -0.6 3,667,440 + +0.4 3,667,559 + -2.6 3,667,667 + Pf6N2E2_3172 0.21 -0.1 3,667,667 + Pf6N2E2_3172 0.21 -0.8 3,667,668 - Pf6N2E2_3172 0.21 +0.4 3,667,715 + Pf6N2E2_3172 0.27 -0.6 3,667,716 - Pf6N2E2_3172 0.27 +0.4 3,667,793 + Pf6N2E2_3172 0.36 -0.7 3,667,793 + Pf6N2E2_3172 0.36 +0.4 3,667,793 + Pf6N2E2_3172 0.36 -2.2 3,667,793 + Pf6N2E2_3172 0.36 +0.4 3,667,793 + Pf6N2E2_3172 0.36 +0.4 3,667,794 - Pf6N2E2_3172 0.36 +0.4 3,667,794 - Pf6N2E2_3172 0.36 -0.6 3,667,794 - Pf6N2E2_3172 0.36 -0.0 3,667,794 - Pf6N2E2_3172 0.36 -1.6 3,667,794 - Pf6N2E2_3172 0.36 -1.5 3,667,794 - Pf6N2E2_3172 0.36 +0.4 3,667,795 + Pf6N2E2_3172 0.37 +0.4 3,667,795 + Pf6N2E2_3172 0.37 -0.6 3,667,796 - Pf6N2E2_3172 0.37 -0.6 3,667,796 - Pf6N2E2_3172 0.37 +0.4 3,667,851 - Pf6N2E2_3172 0.44 -1.2 3,667,916 + Pf6N2E2_3172 0.52 -0.6 3,667,917 - Pf6N2E2_3172 0.52 -0.6 3,667,961 + Pf6N2E2_3172 0.57 +0.4 3,667,962 - Pf6N2E2_3172 0.58 -0.6 3,667,962 - Pf6N2E2_3172 0.58 -1.2 3,667,962 - Pf6N2E2_3172 0.58 +0.4 3,668,298 + -0.0 3,668,298 + -1.6 3,668,299 - -1.2 3,668,299 - +0.4 3,668,299 - -0.6 3,668,383 + +0.4 3,668,384 - +1.4 3,668,384 - +0.4
Or see this region's nucleotide sequence