Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3137 and Pf6N2E2_3138 are separated by 158 nucleotides Pf6N2E2_3138 and Pf6N2E2_3139 are separated by 147 nucleotides
Pf6N2E2_3137 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 3,628,697 to 3,630,406
_3137
Pf6N2E2_3138 - YaeQ protein, at 3,630,565 to 3,631,107
_3138
Pf6N2E2_3139 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,631,255 to 3,632,454
_3139
Position (kb)
3630
3631
3632 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3629.570 kb on - strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.776 kb on + strand, within Pf6N2E2_3137 at 3629.777 kb on - strand, within Pf6N2E2_3137 at 3629.777 kb on - strand, within Pf6N2E2_3137 at 3629.784 kb on + strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.785 kb on - strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.829 kb on + strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3629.830 kb on - strand, within Pf6N2E2_3137 at 3630.001 kb on + strand, within Pf6N2E2_3137 at 3630.002 kb on - strand, within Pf6N2E2_3137 at 3630.008 kb on - strand, within Pf6N2E2_3137 at 3630.081 kb on + strand, within Pf6N2E2_3137 at 3630.081 kb on + strand, within Pf6N2E2_3137 at 3630.082 kb on - strand, within Pf6N2E2_3137 at 3630.082 kb on - strand, within Pf6N2E2_3137 at 3630.105 kb on + strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.106 kb on - strand, within Pf6N2E2_3137 at 3630.330 kb on + strand at 3630.331 kb on - strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.398 kb on + strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.399 kb on - strand at 3630.957 kb on - strand, within Pf6N2E2_3138 at 3630.968 kb on + strand, within Pf6N2E2_3138 at 3630.969 kb on - strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.043 kb on + strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.044 kb on - strand, within Pf6N2E2_3138 at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.085 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.123 kb on + strand at 3631.208 kb on - strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.212 kb on + strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.213 kb on - strand at 3631.222 kb on + strand at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.415 kb on + strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.416 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.418 kb on - strand, within Pf6N2E2_3139 at 3631.712 kb on - strand, within Pf6N2E2_3139 at 3631.864 kb on + strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139 at 3631.865 kb on - strand, within Pf6N2E2_3139
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,629,570 - Pf6N2E2_3137 0.51 +0.4 3,629,776 + Pf6N2E2_3137 0.63 -1.7 3,629,776 + Pf6N2E2_3137 0.63 +0.4 3,629,776 + Pf6N2E2_3137 0.63 -0.2 3,629,777 - Pf6N2E2_3137 0.63 +0.4 3,629,777 - Pf6N2E2_3137 0.63 +1.0 3,629,784 + Pf6N2E2_3137 0.64 +0.4 3,629,785 - Pf6N2E2_3137 0.64 +0.4 3,629,785 - Pf6N2E2_3137 0.64 -1.8 3,629,785 - Pf6N2E2_3137 0.64 -0.6 3,629,785 - Pf6N2E2_3137 0.64 +0.4 3,629,785 - Pf6N2E2_3137 0.64 -0.6 3,629,829 + Pf6N2E2_3137 0.66 -0.6 3,629,829 + Pf6N2E2_3137 0.66 -1.2 3,629,829 + Pf6N2E2_3137 0.66 +0.4 3,629,830 - Pf6N2E2_3137 0.66 -1.6 3,629,830 - Pf6N2E2_3137 0.66 -0.6 3,629,830 - Pf6N2E2_3137 0.66 +0.4 3,629,830 - Pf6N2E2_3137 0.66 +0.4 3,630,001 + Pf6N2E2_3137 0.76 +0.2 3,630,002 - Pf6N2E2_3137 0.76 +0.4 3,630,008 - Pf6N2E2_3137 0.77 -0.4 3,630,081 + Pf6N2E2_3137 0.81 -0.6 3,630,081 + Pf6N2E2_3137 0.81 +0.4 3,630,082 - Pf6N2E2_3137 0.81 +0.4 3,630,082 - Pf6N2E2_3137 0.81 +0.4 3,630,105 + Pf6N2E2_3137 0.82 -1.2 3,630,106 - Pf6N2E2_3137 0.82 -1.2 3,630,106 - Pf6N2E2_3137 0.82 +0.4 3,630,106 - Pf6N2E2_3137 0.82 +0.4 3,630,330 + +0.4 3,630,331 - -1.2 3,630,398 + -0.6 3,630,398 + -2.0 3,630,398 + -0.8 3,630,398 + +0.4 3,630,398 + +0.4 3,630,399 - -0.9 3,630,399 - +0.4 3,630,399 - +0.4 3,630,957 - Pf6N2E2_3138 0.72 +0.4 3,630,968 + Pf6N2E2_3138 0.74 -1.2 3,630,969 - Pf6N2E2_3138 0.74 +0.4 3,631,043 + Pf6N2E2_3138 0.88 -0.6 3,631,043 + Pf6N2E2_3138 0.88 +0.4 3,631,044 - Pf6N2E2_3138 0.88 -0.6 3,631,044 - Pf6N2E2_3138 0.88 -0.9 3,631,044 - Pf6N2E2_3138 0.88 -0.6 3,631,044 - Pf6N2E2_3138 0.88 -3.0 3,631,085 + -0.6 3,631,085 + -1.6 3,631,085 + -0.7 3,631,123 + +0.4 3,631,123 + +0.4 3,631,123 + +0.4 3,631,208 - -1.4 3,631,212 + -0.1 3,631,212 + -0.6 3,631,212 + -0.8 3,631,213 - -2.6 3,631,213 - -0.4 3,631,213 - +0.4 3,631,222 + -1.2 3,631,415 + Pf6N2E2_3139 0.13 -0.8 3,631,415 + Pf6N2E2_3139 0.13 -1.2 3,631,415 + Pf6N2E2_3139 0.13 -0.3 3,631,416 - Pf6N2E2_3139 0.13 +0.0 3,631,416 - Pf6N2E2_3139 0.13 -1.2 3,631,416 - Pf6N2E2_3139 0.13 +0.4 3,631,416 - Pf6N2E2_3139 0.13 +0.4 3,631,416 - Pf6N2E2_3139 0.13 -0.2 3,631,416 - Pf6N2E2_3139 0.13 +0.4 3,631,418 - Pf6N2E2_3139 0.14 +0.4 3,631,418 - Pf6N2E2_3139 0.14 -1.2 3,631,418 - Pf6N2E2_3139 0.14 -0.9 3,631,712 - Pf6N2E2_3139 0.38 +0.4 3,631,864 + Pf6N2E2_3139 0.51 +0.4 3,631,865 - Pf6N2E2_3139 0.51 -1.6 3,631,865 - Pf6N2E2_3139 0.51 +0.4
Or see this region's nucleotide sequence