Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_303

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_302 and Pf6N2E2_303 are separated by 127 nucleotidesPf6N2E2_303 and Pf6N2E2_304 are separated by 88 nucleotides Pf6N2E2_302 - Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107), at 372,395 to 373,789 _302 Pf6N2E2_303 - Glutathione S-transferase, omega (EC 2.5.1.18), at 373,917 to 374,915 _303 Pf6N2E2_304 - Anthranilate phosphoribosyltransferase like (EC 2.4.2.18), at 375,004 to 376,005 _304 Position (kb) 373 374 375Strain fitness (log2 ratio) -1 0 1at 373.049 kb on + strand, within Pf6N2E2_302at 373.193 kb on + strand, within Pf6N2E2_302at 373.193 kb on + strand, within Pf6N2E2_302at 373.440 kb on - strand, within Pf6N2E2_302at 373.538 kb on + strand, within Pf6N2E2_302at 373.538 kb on + strand, within Pf6N2E2_302at 373.538 kb on + strand, within Pf6N2E2_302at 373.538 kb on + strand, within Pf6N2E2_302at 373.538 kb on + strand, within Pf6N2E2_302at 373.539 kb on - strand, within Pf6N2E2_302at 373.539 kb on - strand, within Pf6N2E2_302at 373.539 kb on - strand, within Pf6N2E2_302at 373.539 kb on - strand, within Pf6N2E2_302at 373.755 kb on + strandat 373.755 kb on + strandat 373.787 kb on + strandat 373.788 kb on - strandat 374.035 kb on + strand, within Pf6N2E2_303at 374.035 kb on + strand, within Pf6N2E2_303at 374.035 kb on + strand, within Pf6N2E2_303at 374.036 kb on - strand, within Pf6N2E2_303at 374.036 kb on - strand, within Pf6N2E2_303at 374.036 kb on - strand, within Pf6N2E2_303at 374.036 kb on - strand, within Pf6N2E2_303at 374.039 kb on - strand, within Pf6N2E2_303at 374.039 kb on - strand, within Pf6N2E2_303at 374.039 kb on - strand, within Pf6N2E2_303at 374.052 kb on - strand, within Pf6N2E2_303at 374.092 kb on + strand, within Pf6N2E2_303at 374.167 kb on + strand, within Pf6N2E2_303at 374.167 kb on + strand, within Pf6N2E2_303at 374.168 kb on - strand, within Pf6N2E2_303at 374.225 kb on - strand, within Pf6N2E2_303at 374.225 kb on - strand, within Pf6N2E2_303at 374.239 kb on + strand, within Pf6N2E2_303at 374.308 kb on + strand, within Pf6N2E2_303at 374.309 kb on - strand, within Pf6N2E2_303at 374.366 kb on - strand, within Pf6N2E2_303at 374.545 kb on + strand, within Pf6N2E2_303at 374.546 kb on - strand, within Pf6N2E2_303at 374.566 kb on + strand, within Pf6N2E2_303at 374.656 kb on + strand, within Pf6N2E2_303at 374.670 kb on + strand, within Pf6N2E2_303at 374.670 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.749 kb on + strand, within Pf6N2E2_303at 374.750 kb on - strand, within Pf6N2E2_303at 374.750 kb on - strand, within Pf6N2E2_303at 374.750 kb on - strand, within Pf6N2E2_303at 374.750 kb on - strand, within Pf6N2E2_303at 374.750 kb on - strand, within Pf6N2E2_303at 374.750 kb on - strand, within Pf6N2E2_303at 374.875 kb on + strandat 374.875 kb on + strandat 374.875 kb on + strandat 374.876 kb on - strandat 374.876 kb on - strandat 374.904 kb on + strandat 374.953 kb on + strandat 374.973 kb on - strandat 375.005 kb on + strandat 375.032 kb on + strandat 375.123 kb on - strand, within Pf6N2E2_304at 375.563 kb on - strand, within Pf6N2E2_304at 375.599 kb on + strand, within Pf6N2E2_304at 375.684 kb on - strand, within Pf6N2E2_304at 375.713 kb on + strand, within Pf6N2E2_304at 375.713 kb on + strand, within Pf6N2E2_304at 375.820 kb on + strand, within Pf6N2E2_304

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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373,049 + Pf6N2E2_302 0.47 +0.3
373,193 + Pf6N2E2_302 0.57 -1.7
373,193 + Pf6N2E2_302 0.57 +0.3
373,440 - Pf6N2E2_302 0.75 +0.3
373,538 + Pf6N2E2_302 0.82 +0.3
373,538 + Pf6N2E2_302 0.82 -1.3
373,538 + Pf6N2E2_302 0.82 +0.3
373,538 + Pf6N2E2_302 0.82 +0.3
373,538 + Pf6N2E2_302 0.82 -0.1
373,539 - Pf6N2E2_302 0.82 -0.7
373,539 - Pf6N2E2_302 0.82 +0.3
373,539 - Pf6N2E2_302 0.82 -0.7
373,539 - Pf6N2E2_302 0.82 -1.5
373,755 + -1.7
373,755 + -0.7
373,787 + +0.3
373,788 - +0.3
374,035 + Pf6N2E2_303 0.12 +0.3
374,035 + Pf6N2E2_303 0.12 +0.3
374,035 + Pf6N2E2_303 0.12 +0.4
374,036 - Pf6N2E2_303 0.12 +0.3
374,036 - Pf6N2E2_303 0.12 -0.7
374,036 - Pf6N2E2_303 0.12 +0.3
374,036 - Pf6N2E2_303 0.12 -0.6
374,039 - Pf6N2E2_303 0.12 -1.7
374,039 - Pf6N2E2_303 0.12 -0.3
374,039 - Pf6N2E2_303 0.12 +0.3
374,052 - Pf6N2E2_303 0.14 +0.3
374,092 + Pf6N2E2_303 0.18 +0.3
374,167 + Pf6N2E2_303 0.25 -0.9
374,167 + Pf6N2E2_303 0.25 -0.7
374,168 - Pf6N2E2_303 0.25 +0.3
374,225 - Pf6N2E2_303 0.31 -1.3
374,225 - Pf6N2E2_303 0.31 +0.3
374,239 + Pf6N2E2_303 0.32 +0.3
374,308 + Pf6N2E2_303 0.39 -0.7
374,309 - Pf6N2E2_303 0.39 -1.3
374,366 - Pf6N2E2_303 0.45 +0.3
374,545 + Pf6N2E2_303 0.63 -0.7
374,546 - Pf6N2E2_303 0.63 -1.7
374,566 + Pf6N2E2_303 0.65 +0.3
374,656 + Pf6N2E2_303 0.74 -1.3
374,670 + Pf6N2E2_303 0.75 -1.3
374,670 + Pf6N2E2_303 0.75 -0.7
374,749 + Pf6N2E2_303 0.83 +0.1
374,749 + Pf6N2E2_303 0.83 +0.3
374,749 + Pf6N2E2_303 0.83 +0.3
374,749 + Pf6N2E2_303 0.83 -0.7
374,749 + Pf6N2E2_303 0.83 -0.3
374,749 + Pf6N2E2_303 0.83 +0.3
374,749 + Pf6N2E2_303 0.83 -0.9
374,749 + Pf6N2E2_303 0.83 +0.3
374,749 + Pf6N2E2_303 0.83 +0.3
374,749 + Pf6N2E2_303 0.83 +0.3
374,750 - Pf6N2E2_303 0.83 -0.4
374,750 - Pf6N2E2_303 0.83 -1.6
374,750 - Pf6N2E2_303 0.83 +0.3
374,750 - Pf6N2E2_303 0.83 -1.6
374,750 - Pf6N2E2_303 0.83 +0.3
374,750 - Pf6N2E2_303 0.83 +0.3
374,875 + +0.3
374,875 + +0.3
374,875 + -0.8
374,876 - -0.7
374,876 - +0.3
374,904 + -0.7
374,953 + +0.3
374,973 - -0.1
375,005 + -0.6
375,032 + +0.3
375,123 - Pf6N2E2_304 0.12 +0.3
375,563 - Pf6N2E2_304 0.56 +0.3
375,599 + Pf6N2E2_304 0.59 +0.3
375,684 - Pf6N2E2_304 0.68 +0.3
375,713 + Pf6N2E2_304 0.71 -0.9
375,713 + Pf6N2E2_304 0.71 +0.3
375,820 + Pf6N2E2_304 0.81 -0.7

Or see this region's nucleotide sequence