Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2856 and Pf6N2E2_2857 are separated by 140 nucleotides Pf6N2E2_2857 and Pf6N2E2_2858 are separated by 32 nucleotides Pf6N2E2_2858 and Pf6N2E2_2859 are separated by 11 nucleotides Pf6N2E2_2859 and Pf6N2E2_2860 are separated by 80 nucleotides
Pf6N2E2_2856 - Ketosteroid isomerase homolog, at 3,348,176 to 3,348,610
_2856
Pf6N2E2_2857 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 3,348,751 to 3,349,236
_2857
Pf6N2E2_2858 - Phosphohydrolase (MutT/nudix family protein), at 3,349,269 to 3,349,877
_2858
Pf6N2E2_2859 - Bacterial transcription activator, effector binding protein, at 3,349,889 to 3,350,377
_2859
Pf6N2E2_2860 - Putative threonine efflux protein, at 3,350,458 to 3,351,120
_2860
Position (kb)
3349
3350 Strain fitness (log2 ratio)
-2
-1
0
1 at 3348.337 kb on - strand, within Pf6N2E2_2856 at 3348.337 kb on - strand, within Pf6N2E2_2856 at 3348.513 kb on + strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.514 kb on - strand, within Pf6N2E2_2856 at 3348.561 kb on + strand, within Pf6N2E2_2856 at 3348.562 kb on - strand, within Pf6N2E2_2856 at 3348.604 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.722 kb on + strand at 3348.723 kb on - strand at 3349.042 kb on + strand, within Pf6N2E2_2857 at 3349.153 kb on + strand, within Pf6N2E2_2857 at 3349.153 kb on + strand, within Pf6N2E2_2857 at 3349.154 kb on - strand, within Pf6N2E2_2857 at 3349.154 kb on - strand, within Pf6N2E2_2857 at 3349.190 kb on - strand at 3349.190 kb on - strand at 3349.252 kb on - strand at 3349.305 kb on + strand at 3349.330 kb on + strand, within Pf6N2E2_2858 at 3349.330 kb on + strand, within Pf6N2E2_2858 at 3349.362 kb on + strand, within Pf6N2E2_2858 at 3349.363 kb on - strand, within Pf6N2E2_2858 at 3349.363 kb on - strand, within Pf6N2E2_2858 at 3349.428 kb on - strand, within Pf6N2E2_2858 at 3349.833 kb on + strand at 3349.833 kb on + strand at 3349.834 kb on - strand at 3349.834 kb on - strand at 3349.873 kb on - strand at 3349.873 kb on - strand at 3349.959 kb on + strand, within Pf6N2E2_2859 at 3349.990 kb on + strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3349.991 kb on - strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.025 kb on + strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.026 kb on - strand, within Pf6N2E2_2859 at 3350.043 kb on - strand, within Pf6N2E2_2859 at 3350.080 kb on - strand, within Pf6N2E2_2859 at 3350.082 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.157 kb on + strand, within Pf6N2E2_2859 at 3350.158 kb on - strand, within Pf6N2E2_2859 at 3350.199 kb on + strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.200 kb on - strand, within Pf6N2E2_2859 at 3350.314 kb on + strand, within Pf6N2E2_2859 at 3350.355 kb on + strand at 3350.420 kb on + strand at 3350.420 kb on + strand at 3350.420 kb on + strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.421 kb on - strand at 3350.494 kb on + strand at 3350.526 kb on - strand, within Pf6N2E2_2860 at 3350.712 kb on + strand, within Pf6N2E2_2860 at 3350.713 kb on - strand, within Pf6N2E2_2860 at 3350.718 kb on + strand, within Pf6N2E2_2860 at 3350.847 kb on - strand, within Pf6N2E2_2860
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,348,337 - Pf6N2E2_2856 0.37 -0.1 3,348,337 - Pf6N2E2_2856 0.37 +0.4 3,348,513 + Pf6N2E2_2856 0.77 +0.4 3,348,514 - Pf6N2E2_2856 0.78 -0.1 3,348,514 - Pf6N2E2_2856 0.78 +0.4 3,348,514 - Pf6N2E2_2856 0.78 -0.6 3,348,514 - Pf6N2E2_2856 0.78 -1.2 3,348,514 - Pf6N2E2_2856 0.78 -1.2 3,348,561 + Pf6N2E2_2856 0.89 +0.4 3,348,562 - Pf6N2E2_2856 0.89 -0.7 3,348,604 + +0.4 3,348,722 + -0.6 3,348,722 + +0.4 3,348,722 + -1.2 3,348,722 + -1.2 3,348,722 + +0.4 3,348,723 - +0.4 3,349,042 + Pf6N2E2_2857 0.60 -0.4 3,349,153 + Pf6N2E2_2857 0.83 -0.6 3,349,153 + Pf6N2E2_2857 0.83 +0.4 3,349,154 - Pf6N2E2_2857 0.83 -0.6 3,349,154 - Pf6N2E2_2857 0.83 -1.6 3,349,190 - +0.6 3,349,190 - +0.4 3,349,252 - -1.2 3,349,305 + -0.6 3,349,330 + Pf6N2E2_2858 0.10 +0.4 3,349,330 + Pf6N2E2_2858 0.10 -1.2 3,349,362 + Pf6N2E2_2858 0.15 -1.6 3,349,363 - Pf6N2E2_2858 0.15 +1.0 3,349,363 - Pf6N2E2_2858 0.15 -1.6 3,349,428 - Pf6N2E2_2858 0.26 -1.8 3,349,833 + +0.4 3,349,833 + +0.4 3,349,834 - -0.4 3,349,834 - +0.4 3,349,873 - +0.4 3,349,873 - +0.4 3,349,959 + Pf6N2E2_2859 0.14 -0.6 3,349,990 + Pf6N2E2_2859 0.21 +0.4 3,349,991 - Pf6N2E2_2859 0.21 -0.9 3,349,991 - Pf6N2E2_2859 0.21 -0.6 3,349,991 - Pf6N2E2_2859 0.21 +0.4 3,350,025 + Pf6N2E2_2859 0.28 +0.4 3,350,025 + Pf6N2E2_2859 0.28 +0.0 3,350,025 + Pf6N2E2_2859 0.28 -0.5 3,350,025 + Pf6N2E2_2859 0.28 -1.3 3,350,026 - Pf6N2E2_2859 0.28 -0.6 3,350,026 - Pf6N2E2_2859 0.28 -1.2 3,350,026 - Pf6N2E2_2859 0.28 +0.4 3,350,026 - Pf6N2E2_2859 0.28 +0.4 3,350,026 - Pf6N2E2_2859 0.28 -0.9 3,350,026 - Pf6N2E2_2859 0.28 -0.6 3,350,043 - Pf6N2E2_2859 0.31 +0.4 3,350,080 - Pf6N2E2_2859 0.39 +0.4 3,350,082 + Pf6N2E2_2859 0.39 -0.2 3,350,157 + Pf6N2E2_2859 0.55 -1.2 3,350,157 + Pf6N2E2_2859 0.55 -0.6 3,350,158 - Pf6N2E2_2859 0.55 -0.6 3,350,199 + Pf6N2E2_2859 0.63 -1.2 3,350,200 - Pf6N2E2_2859 0.64 -0.6 3,350,200 - Pf6N2E2_2859 0.64 -0.6 3,350,200 - Pf6N2E2_2859 0.64 -0.9 3,350,200 - Pf6N2E2_2859 0.64 -0.0 3,350,314 + Pf6N2E2_2859 0.87 -0.8 3,350,355 + +0.4 3,350,420 + +0.4 3,350,420 + +0.4 3,350,420 + +0.4 3,350,421 - -2.2 3,350,421 - +0.4 3,350,421 - +0.4 3,350,421 - +0.4 3,350,421 - -0.6 3,350,421 - -0.6 3,350,494 + +0.4 3,350,526 - Pf6N2E2_2860 0.10 -1.0 3,350,712 + Pf6N2E2_2860 0.38 +0.4 3,350,713 - Pf6N2E2_2860 0.38 +1.1 3,350,718 + Pf6N2E2_2860 0.39 -0.6 3,350,847 - Pf6N2E2_2860 0.59 +0.4
Or see this region's nucleotide sequence