Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2841 and Pf6N2E2_2842 are separated by 169 nucleotides Pf6N2E2_2842 and Pf6N2E2_2843 are separated by 144 nucleotides Pf6N2E2_2843 and Pf6N2E2_2844 are separated by 94 nucleotides Pf6N2E2_2844 and Pf6N2E2_2845 are separated by 228 nucleotides
Pf6N2E2_2841 - Low-affinity inorganic phosphate transporter, at 3,330,894 to 3,332,330
_2841
Pf6N2E2_2842 - hypothetical protein, at 3,332,500 to 3,332,616
_2842
Pf6N2E2_2843 - FIG00959308: hypothetical protein, at 3,332,761 to 3,332,922
_2843
Pf6N2E2_2844 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 3,333,017 to 3,333,358
_2844
Pf6N2E2_2845 - RNA polymerase associated protein RapA (EC 3.6.1.-), at 3,333,587 to 3,336,433
_2845
Position (kb)
3333
3334 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3332.380 kb on + strand at 3332.386 kb on + strand at 3332.387 kb on - strand at 3332.501 kb on + strand at 3332.540 kb on - strand, within Pf6N2E2_2842 at 3332.560 kb on + strand, within Pf6N2E2_2842 at 3332.731 kb on - strand at 3332.907 kb on + strand at 3332.997 kb on + strand at 3332.998 kb on - strand at 3333.282 kb on - strand, within Pf6N2E2_2844 at 3333.282 kb on - strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.323 kb on - strand, within Pf6N2E2_2844 at 3333.326 kb on + strand at 3333.327 kb on - strand at 3333.358 kb on + strand at 3333.358 kb on + strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.359 kb on - strand at 3333.485 kb on + strand at 3333.485 kb on + strand at 3333.486 kb on - strand at 3333.486 kb on - strand at 3333.486 kb on - strand at 3333.506 kb on + strand at 3333.515 kb on + strand at 3333.515 kb on + strand at 3333.515 kb on + strand at 3333.515 kb on + strand at 3333.516 kb on - strand at 3333.516 kb on - strand at 3333.519 kb on + strand at 3333.519 kb on + strand at 3333.519 kb on + strand at 3333.519 kb on + strand at 3333.520 kb on - strand at 3333.599 kb on + strand at 3333.649 kb on - strand at 3333.719 kb on + strand at 3333.720 kb on - strand at 3333.750 kb on + strand at 3333.751 kb on - strand at 3334.080 kb on + strand, within Pf6N2E2_2845 at 3334.080 kb on + strand, within Pf6N2E2_2845 at 3334.081 kb on - strand, within Pf6N2E2_2845
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,332,380 + +0.4 3,332,386 + -1.2 3,332,387 - -2.1 3,332,501 + +0.4 3,332,540 - Pf6N2E2_2842 0.34 -0.6 3,332,560 + Pf6N2E2_2842 0.51 -0.2 3,332,731 - -0.3 3,332,907 + -0.4 3,332,997 + +0.4 3,332,998 - -1.2 3,333,282 - Pf6N2E2_2844 0.77 -1.6 3,333,282 - Pf6N2E2_2844 0.77 -0.6 3,333,322 + Pf6N2E2_2844 0.89 -1.2 3,333,322 + Pf6N2E2_2844 0.89 -1.6 3,333,322 + Pf6N2E2_2844 0.89 -0.6 3,333,322 + Pf6N2E2_2844 0.89 -0.6 3,333,322 + Pf6N2E2_2844 0.89 -0.6 3,333,322 + Pf6N2E2_2844 0.89 +0.4 3,333,322 + Pf6N2E2_2844 0.89 +0.4 3,333,322 + Pf6N2E2_2844 0.89 -1.2 3,333,323 - Pf6N2E2_2844 0.89 +0.4 3,333,326 + +0.4 3,333,327 - -1.2 3,333,358 + +0.4 3,333,358 + +0.4 3,333,359 - +0.4 3,333,359 - -0.8 3,333,359 - +0.4 3,333,359 - +0.4 3,333,359 - -1.9 3,333,359 - +0.4 3,333,359 - +0.4 3,333,359 - -0.6 3,333,359 - +1.8 3,333,485 + +1.0 3,333,485 + +0.3 3,333,486 - +0.4 3,333,486 - +0.4 3,333,486 - -0.0 3,333,506 + +0.4 3,333,515 + +0.4 3,333,515 + -0.6 3,333,515 + -1.9 3,333,515 + -0.6 3,333,516 - -0.3 3,333,516 - +0.3 3,333,519 + -1.2 3,333,519 + +0.4 3,333,519 + +0.4 3,333,519 + -0.0 3,333,520 - -0.6 3,333,599 + +0.4 3,333,649 - +0.4 3,333,719 + -0.1 3,333,720 - +0.4 3,333,750 + +0.4 3,333,751 - +0.4 3,334,080 + Pf6N2E2_2845 0.17 -1.6 3,334,080 + Pf6N2E2_2845 0.17 -0.6 3,334,081 - Pf6N2E2_2845 0.17 -1.6
Or see this region's nucleotide sequence