Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2655 and Pf6N2E2_2656 are separated by 9 nucleotides Pf6N2E2_2656 and Pf6N2E2_2657 are separated by 52 nucleotides
Pf6N2E2_2655 - Agmatinase (EC 3.5.3.11), at 3,127,840 to 3,128,790
_2655
Pf6N2E2_2656 - Undecaprenyl pyrophosphate synthase, at 3,128,800 to 3,129,207
_2656
Pf6N2E2_2657 - Cytosine/purine/uracil/thiamine/allantoin permease family protein, at 3,129,260 to 3,130,771
_2657
Position (kb)
3128
3129
3130 Strain fitness (log2 ratio)
-1
0
1 at 3127.817 kb on - strand at 3127.817 kb on - strand at 3127.817 kb on - strand at 3127.817 kb on - strand at 3128.051 kb on - strand, within Pf6N2E2_2655 at 3128.051 kb on - strand, within Pf6N2E2_2655 at 3128.051 kb on - strand, within Pf6N2E2_2655 at 3128.164 kb on + strand, within Pf6N2E2_2655 at 3128.165 kb on - strand, within Pf6N2E2_2655 at 3128.545 kb on + strand, within Pf6N2E2_2655 at 3128.545 kb on + strand, within Pf6N2E2_2655 at 3128.546 kb on - strand, within Pf6N2E2_2655 at 3128.546 kb on - strand, within Pf6N2E2_2655 at 3128.687 kb on + strand, within Pf6N2E2_2655 at 3128.688 kb on - strand, within Pf6N2E2_2655 at 3128.688 kb on - strand, within Pf6N2E2_2655 at 3128.750 kb on - strand at 3128.875 kb on + strand, within Pf6N2E2_2656 at 3128.875 kb on + strand, within Pf6N2E2_2656 at 3128.875 kb on + strand, within Pf6N2E2_2656 at 3128.951 kb on - strand, within Pf6N2E2_2656 at 3128.951 kb on - strand, within Pf6N2E2_2656 at 3129.149 kb on + strand, within Pf6N2E2_2656 at 3129.150 kb on - strand, within Pf6N2E2_2656 at 3129.205 kb on + strand at 3129.243 kb on + strand at 3129.243 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.265 kb on + strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.266 kb on - strand at 3129.272 kb on - strand at 3129.383 kb on - strand at 3129.749 kb on + strand, within Pf6N2E2_2657 at 3129.750 kb on - strand, within Pf6N2E2_2657 at 3129.750 kb on - strand, within Pf6N2E2_2657 at 3129.750 kb on - strand, within Pf6N2E2_2657 at 3129.789 kb on + strand, within Pf6N2E2_2657 at 3129.972 kb on - strand, within Pf6N2E2_2657 at 3129.972 kb on - strand, within Pf6N2E2_2657 at 3130.097 kb on + strand, within Pf6N2E2_2657 at 3130.097 kb on + strand, within Pf6N2E2_2657 at 3130.115 kb on + strand, within Pf6N2E2_2657 at 3130.115 kb on + strand, within Pf6N2E2_2657 at 3130.115 kb on + strand, within Pf6N2E2_2657 at 3130.115 kb on + strand, within Pf6N2E2_2657 at 3130.116 kb on - strand, within Pf6N2E2_2657
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,127,817 - +0.3 3,127,817 - -1.2 3,127,817 - -0.9 3,127,817 - +0.3 3,128,051 - Pf6N2E2_2655 0.22 +0.3 3,128,051 - Pf6N2E2_2655 0.22 +0.3 3,128,051 - Pf6N2E2_2655 0.22 +0.3 3,128,164 + Pf6N2E2_2655 0.34 +0.3 3,128,165 - Pf6N2E2_2655 0.34 +0.3 3,128,545 + Pf6N2E2_2655 0.74 -1.2 3,128,545 + Pf6N2E2_2655 0.74 -0.9 3,128,546 - Pf6N2E2_2655 0.74 -1.2 3,128,546 - Pf6N2E2_2655 0.74 -1.3 3,128,687 + Pf6N2E2_2655 0.89 -0.7 3,128,688 - Pf6N2E2_2655 0.89 -0.5 3,128,688 - Pf6N2E2_2655 0.89 -0.7 3,128,750 - -0.2 3,128,875 + Pf6N2E2_2656 0.18 -0.7 3,128,875 + Pf6N2E2_2656 0.18 -1.7 3,128,875 + Pf6N2E2_2656 0.18 -0.7 3,128,951 - Pf6N2E2_2656 0.37 +0.3 3,128,951 - Pf6N2E2_2656 0.37 -1.7 3,129,149 + Pf6N2E2_2656 0.86 +0.3 3,129,150 - Pf6N2E2_2656 0.86 -0.7 3,129,205 + +0.3 3,129,243 + +0.3 3,129,243 + -0.7 3,129,265 + +0.3 3,129,265 + +0.3 3,129,265 + +0.3 3,129,265 + +0.1 3,129,265 + +0.3 3,129,266 - +0.3 3,129,266 - +0.3 3,129,266 - +0.3 3,129,266 - +0.3 3,129,266 - -1.2 3,129,266 - +1.3 3,129,266 - +0.3 3,129,272 - -1.0 3,129,383 - +1.3 3,129,749 + Pf6N2E2_2657 0.32 +0.3 3,129,750 - Pf6N2E2_2657 0.32 +0.3 3,129,750 - Pf6N2E2_2657 0.32 +0.3 3,129,750 - Pf6N2E2_2657 0.32 +0.3 3,129,789 + Pf6N2E2_2657 0.35 -0.2 3,129,972 - Pf6N2E2_2657 0.47 +0.3 3,129,972 - Pf6N2E2_2657 0.47 +0.3 3,130,097 + Pf6N2E2_2657 0.55 +0.3 3,130,097 + Pf6N2E2_2657 0.55 -0.7 3,130,115 + Pf6N2E2_2657 0.57 +0.3 3,130,115 + Pf6N2E2_2657 0.57 +0.8 3,130,115 + Pf6N2E2_2657 0.57 +0.3 3,130,115 + Pf6N2E2_2657 0.57 -1.2 3,130,116 - Pf6N2E2_2657 0.57 +0.3
Or see this region's nucleotide sequence