Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2552

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2550 and Pf6N2E2_2551 are separated by 389 nucleotidesPf6N2E2_2551 and Pf6N2E2_2552 are separated by 14 nucleotidesPf6N2E2_2552 and Pf6N2E2_2553 overlap by 8 nucleotidesPf6N2E2_2553 and Pf6N2E2_2554 overlap by 4 nucleotidesPf6N2E2_2554 and Pf6N2E2_2555 overlap by 4 nucleotides Pf6N2E2_2550 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 3,009,761 to 3,011,854 _2550 Pf6N2E2_2551 - Cytochrome c heme lyase subunit CcmH, at 3,012,244 to 3,012,651 _2551 Pf6N2E2_2552 - Cytochrome c heme lyase subunit CcmH, at 3,012,666 to 3,013,868 _2552 Pf6N2E2_2553 - Cytochrome c heme lyase subunit CcmL, at 3,013,861 to 3,014,334 _2553 Pf6N2E2_2554 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 3,014,331 to 3,014,867 _2554 Pf6N2E2_2555 - Cytochrome c heme lyase subunit CcmF, at 3,014,864 to 3,016,852 _2555 Position (kb) 3012 3013 3014Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3011.708 kb on - strandat 3011.709 kb on + strandat 3011.709 kb on + strandat 3011.710 kb on - strandat 3011.710 kb on - strandat 3011.710 kb on - strandat 3011.710 kb on - strandat 3011.776 kb on - strandat 3011.776 kb on - strandat 3011.818 kb on + strandat 3011.819 kb on - strandat 3011.819 kb on - strandat 3011.819 kb on - strandat 3011.819 kb on - strandat 3011.821 kb on + strandat 3011.822 kb on - strandat 3011.822 kb on - strandat 3011.984 kb on + strandat 3011.984 kb on + strandat 3011.984 kb on + strandat 3011.984 kb on + strandat 3011.984 kb on + strandat 3012.031 kb on - strandat 3012.031 kb on - strandat 3012.054 kb on - strandat 3012.122 kb on - strandat 3012.122 kb on - strandat 3012.176 kb on + strandat 3012.176 kb on + strandat 3012.177 kb on - strandat 3012.246 kb on - strandat 3012.246 kb on - strandat 3012.246 kb on - strandat 3012.347 kb on + strand, within Pf6N2E2_2551at 3012.350 kb on + strand, within Pf6N2E2_2551at 3012.350 kb on + strand, within Pf6N2E2_2551at 3012.351 kb on - strand, within Pf6N2E2_2551at 3012.351 kb on - strand, within Pf6N2E2_2551at 3012.351 kb on - strand, within Pf6N2E2_2551at 3012.351 kb on - strand, within Pf6N2E2_2551at 3012.351 kb on - strand, within Pf6N2E2_2551at 3012.395 kb on + strand, within Pf6N2E2_2551at 3012.395 kb on + strand, within Pf6N2E2_2551at 3012.395 kb on + strand, within Pf6N2E2_2551at 3012.395 kb on + strand, within Pf6N2E2_2551at 3012.395 kb on + strand, within Pf6N2E2_2551at 3012.396 kb on - strand, within Pf6N2E2_2551at 3012.396 kb on - strand, within Pf6N2E2_2551at 3012.396 kb on - strand, within Pf6N2E2_2551at 3012.396 kb on - strand, within Pf6N2E2_2551at 3012.459 kb on + strand, within Pf6N2E2_2551at 3012.459 kb on + strand, within Pf6N2E2_2551at 3012.459 kb on + strand, within Pf6N2E2_2551at 3012.633 kb on + strandat 3012.634 kb on - strandat 3012.634 kb on - strandat 3012.634 kb on - strandat 3012.668 kb on - strandat 3013.160 kb on - strand, within Pf6N2E2_2552at 3013.257 kb on - strand, within Pf6N2E2_2552at 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.390 kb on + strandat 3013.390 kb on + strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.391 kb on - strand, within Pf6N2E2_2552at 3013.394 kb on + strand, within Pf6N2E2_2552at 3013.408 kb on + strand, within Pf6N2E2_2552at 3013.408 kb on + strand, within Pf6N2E2_2552at 3013.409 kb on - strand, within Pf6N2E2_2552at 3013.541 kb on - strand, within Pf6N2E2_2552at 3013.541 kb on - strand, within Pf6N2E2_2552at 3013.741 kb on + strand, within Pf6N2E2_2552at 3013.742 kb on - strand, within Pf6N2E2_2552at 3014.363 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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3,011,708 - +1.4
3,011,709 + +0.4
3,011,709 + +0.4
3,011,710 - -0.6
3,011,710 - -0.6
3,011,710 - -0.6
3,011,710 - -0.6
3,011,776 - +0.4
3,011,776 - -0.6
3,011,818 + +0.4
3,011,819 - -0.6
3,011,819 - -1.2
3,011,819 - -0.1
3,011,819 - -1.2
3,011,821 + +0.4
3,011,822 - +0.4
3,011,822 - -0.4
3,011,984 + -0.6
3,011,984 + +0.6
3,011,984 + +0.2
3,011,984 + +0.4
3,011,984 + -0.6
3,012,031 - +0.4
3,012,031 - -0.5
3,012,054 - +0.4
3,012,122 - -0.6
3,012,122 - +0.4
3,012,176 + +0.4
3,012,176 + +0.4
3,012,177 - -0.6
3,012,246 - +0.4
3,012,246 - -1.2
3,012,246 - -0.6
3,012,347 + Pf6N2E2_2551 0.25 -0.7
3,012,350 + Pf6N2E2_2551 0.26 -1.6
3,012,350 + Pf6N2E2_2551 0.26 -1.2
3,012,351 - Pf6N2E2_2551 0.26 +0.4
3,012,351 - Pf6N2E2_2551 0.26 -1.2
3,012,351 - Pf6N2E2_2551 0.26 -0.3
3,012,351 - Pf6N2E2_2551 0.26 +0.4
3,012,351 - Pf6N2E2_2551 0.26 -1.2
3,012,395 + Pf6N2E2_2551 0.37 -0.6
3,012,395 + Pf6N2E2_2551 0.37 -1.2
3,012,395 + Pf6N2E2_2551 0.37 +0.4
3,012,395 + Pf6N2E2_2551 0.37 +0.4
3,012,395 + Pf6N2E2_2551 0.37 +0.4
3,012,396 - Pf6N2E2_2551 0.37 -0.6
3,012,396 - Pf6N2E2_2551 0.37 +0.4
3,012,396 - Pf6N2E2_2551 0.37 +0.4
3,012,396 - Pf6N2E2_2551 0.37 +0.4
3,012,459 + Pf6N2E2_2551 0.53 -1.0
3,012,459 + Pf6N2E2_2551 0.53 -0.6
3,012,459 + Pf6N2E2_2551 0.53 -1.2
3,012,633 + -0.6
3,012,634 - +0.1
3,012,634 - -0.8
3,012,634 - +0.4
3,012,668 - -0.6
3,013,160 - Pf6N2E2_2552 0.41 +0.4
3,013,257 - Pf6N2E2_2552 0.49 -2.8
3,013,390 + Pf6N2E2_2552 0.60 -2.3
3,013,390 + Pf6N2E2_2552 0.60 -2.4
3,013,390 + Pf6N2E2_2552 0.60 -2.1
3,013,390 + -0.2
3,013,390 + Pf6N2E2_2552 0.60 -2.0
3,013,391 - Pf6N2E2_2552 0.60 -1.7
3,013,391 - Pf6N2E2_2552 0.60 -1.5
3,013,391 - Pf6N2E2_2552 0.60 +0.7
3,013,391 - Pf6N2E2_2552 0.60 -1.7
3,013,391 - Pf6N2E2_2552 0.60 -1.8
3,013,394 + Pf6N2E2_2552 0.61 -1.6
3,013,408 + Pf6N2E2_2552 0.62 -1.0
3,013,408 + Pf6N2E2_2552 0.62 -1.3
3,013,409 - Pf6N2E2_2552 0.62 -1.1
3,013,541 - Pf6N2E2_2552 0.73 -5.6
3,013,541 - Pf6N2E2_2552 0.73 -5.5
3,013,741 + Pf6N2E2_2552 0.89 -5.3
3,013,742 - Pf6N2E2_2552 0.89 -4.5
3,014,363 - -5.5

Or see this region's nucleotide sequence