Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2550 and Pf6N2E2_2551 are separated by 389 nucleotides Pf6N2E2_2551 and Pf6N2E2_2552 are separated by 14 nucleotides Pf6N2E2_2552 and Pf6N2E2_2553 overlap by 8 nucleotides Pf6N2E2_2553 and Pf6N2E2_2554 overlap by 4 nucleotides Pf6N2E2_2554 and Pf6N2E2_2555 overlap by 4 nucleotides
Pf6N2E2_2550 - Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily, at 3,009,761 to 3,011,854
_2550
Pf6N2E2_2551 - Cytochrome c heme lyase subunit CcmH, at 3,012,244 to 3,012,651
_2551
Pf6N2E2_2552 - Cytochrome c heme lyase subunit CcmH, at 3,012,666 to 3,013,868
_2552
Pf6N2E2_2553 - Cytochrome c heme lyase subunit CcmL, at 3,013,861 to 3,014,334
_2553
Pf6N2E2_2554 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 3,014,331 to 3,014,867
_2554
Pf6N2E2_2555 - Cytochrome c heme lyase subunit CcmF, at 3,014,864 to 3,016,852
_2555
Position (kb)
3012
3013
3014 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3011.708 kb on - strand at 3011.709 kb on + strand at 3011.709 kb on + strand at 3011.710 kb on - strand at 3011.710 kb on - strand at 3011.710 kb on - strand at 3011.710 kb on - strand at 3011.776 kb on - strand at 3011.776 kb on - strand at 3011.818 kb on + strand at 3011.819 kb on - strand at 3011.819 kb on - strand at 3011.819 kb on - strand at 3011.819 kb on - strand at 3011.821 kb on + strand at 3011.822 kb on - strand at 3011.822 kb on - strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3011.984 kb on + strand at 3012.031 kb on - strand at 3012.031 kb on - strand at 3012.054 kb on - strand at 3012.122 kb on - strand at 3012.122 kb on - strand at 3012.176 kb on + strand at 3012.176 kb on + strand at 3012.177 kb on - strand at 3012.246 kb on - strand at 3012.246 kb on - strand at 3012.246 kb on - strand at 3012.347 kb on + strand, within Pf6N2E2_2551 at 3012.350 kb on + strand, within Pf6N2E2_2551 at 3012.350 kb on + strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.351 kb on - strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.395 kb on + strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.396 kb on - strand, within Pf6N2E2_2551 at 3012.459 kb on + strand, within Pf6N2E2_2551 at 3012.459 kb on + strand, within Pf6N2E2_2551 at 3012.459 kb on + strand, within Pf6N2E2_2551 at 3012.633 kb on + strand at 3012.634 kb on - strand at 3012.634 kb on - strand at 3012.634 kb on - strand at 3012.668 kb on - strand at 3013.160 kb on - strand, within Pf6N2E2_2552 at 3013.257 kb on - strand, within Pf6N2E2_2552 at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.390 kb on + strand at 3013.390 kb on + strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.391 kb on - strand, within Pf6N2E2_2552 at 3013.394 kb on + strand, within Pf6N2E2_2552 at 3013.408 kb on + strand, within Pf6N2E2_2552 at 3013.408 kb on + strand, within Pf6N2E2_2552 at 3013.409 kb on - strand, within Pf6N2E2_2552 at 3013.541 kb on - strand, within Pf6N2E2_2552 at 3013.541 kb on - strand, within Pf6N2E2_2552 at 3013.741 kb on + strand, within Pf6N2E2_2552 at 3013.742 kb on - strand, within Pf6N2E2_2552 at 3014.363 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 3,011,708 - +1.4 3,011,709 + +0.4 3,011,709 + +0.4 3,011,710 - -0.6 3,011,710 - -0.6 3,011,710 - -0.6 3,011,710 - -0.6 3,011,776 - +0.4 3,011,776 - -0.6 3,011,818 + +0.4 3,011,819 - -0.6 3,011,819 - -1.2 3,011,819 - -0.1 3,011,819 - -1.2 3,011,821 + +0.4 3,011,822 - +0.4 3,011,822 - -0.4 3,011,984 + -0.6 3,011,984 + +0.6 3,011,984 + +0.2 3,011,984 + +0.4 3,011,984 + -0.6 3,012,031 - +0.4 3,012,031 - -0.5 3,012,054 - +0.4 3,012,122 - -0.6 3,012,122 - +0.4 3,012,176 + +0.4 3,012,176 + +0.4 3,012,177 - -0.6 3,012,246 - +0.4 3,012,246 - -1.2 3,012,246 - -0.6 3,012,347 + Pf6N2E2_2551 0.25 -0.7 3,012,350 + Pf6N2E2_2551 0.26 -1.6 3,012,350 + Pf6N2E2_2551 0.26 -1.2 3,012,351 - Pf6N2E2_2551 0.26 +0.4 3,012,351 - Pf6N2E2_2551 0.26 -1.2 3,012,351 - Pf6N2E2_2551 0.26 -0.3 3,012,351 - Pf6N2E2_2551 0.26 +0.4 3,012,351 - Pf6N2E2_2551 0.26 -1.2 3,012,395 + Pf6N2E2_2551 0.37 -0.6 3,012,395 + Pf6N2E2_2551 0.37 -1.2 3,012,395 + Pf6N2E2_2551 0.37 +0.4 3,012,395 + Pf6N2E2_2551 0.37 +0.4 3,012,395 + Pf6N2E2_2551 0.37 +0.4 3,012,396 - Pf6N2E2_2551 0.37 -0.6 3,012,396 - Pf6N2E2_2551 0.37 +0.4 3,012,396 - Pf6N2E2_2551 0.37 +0.4 3,012,396 - Pf6N2E2_2551 0.37 +0.4 3,012,459 + Pf6N2E2_2551 0.53 -1.0 3,012,459 + Pf6N2E2_2551 0.53 -0.6 3,012,459 + Pf6N2E2_2551 0.53 -1.2 3,012,633 + -0.6 3,012,634 - +0.1 3,012,634 - -0.8 3,012,634 - +0.4 3,012,668 - -0.6 3,013,160 - Pf6N2E2_2552 0.41 +0.4 3,013,257 - Pf6N2E2_2552 0.49 -2.8 3,013,390 + Pf6N2E2_2552 0.60 -2.3 3,013,390 + Pf6N2E2_2552 0.60 -2.4 3,013,390 + Pf6N2E2_2552 0.60 -2.1 3,013,390 + -0.2 3,013,390 + Pf6N2E2_2552 0.60 -2.0 3,013,391 - Pf6N2E2_2552 0.60 -1.7 3,013,391 - Pf6N2E2_2552 0.60 -1.5 3,013,391 - Pf6N2E2_2552 0.60 +0.7 3,013,391 - Pf6N2E2_2552 0.60 -1.7 3,013,391 - Pf6N2E2_2552 0.60 -1.8 3,013,394 + Pf6N2E2_2552 0.61 -1.6 3,013,408 + Pf6N2E2_2552 0.62 -1.0 3,013,408 + Pf6N2E2_2552 0.62 -1.3 3,013,409 - Pf6N2E2_2552 0.62 -1.1 3,013,541 - Pf6N2E2_2552 0.73 -5.6 3,013,541 - Pf6N2E2_2552 0.73 -5.5 3,013,741 + Pf6N2E2_2552 0.89 -5.3 3,013,742 - Pf6N2E2_2552 0.89 -4.5 3,014,363 - -5.5
Or see this region's nucleotide sequence