Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2365 and Pf6N2E2_2366 overlap by 8 nucleotides Pf6N2E2_2366 and Pf6N2E2_2367 are separated by 541 nucleotides Pf6N2E2_2367 and Pf6N2E2_2368 overlap by 4 nucleotides Pf6N2E2_2368 and Pf6N2E2_2369 overlap by 17 nucleotides
Pf6N2E2_2365 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 2,799,421 to 2,800,080
_2365
Pf6N2E2_2366 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 2,800,073 to 2,800,453
_2366
Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870
_2367
Pf6N2E2_2368 - Surface presentation of antigens protein SpaM, at 2,801,867 to 2,802,322
_2368
Pf6N2E2_2369 - Flagellum-specific ATP synthase FliI, at 2,802,306 to 2,803,556
_2369
Position (kb)
2800
2801
2802 Strain fitness (log2 ratio)
-2
-1
0
1 at 2800.004 kb on + strand, within Pf6N2E2_2365 at 2800.005 kb on - strand, within Pf6N2E2_2365 at 2800.370 kb on + strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.384 kb on + strand, within Pf6N2E2_2366 at 2800.385 kb on - strand, within Pf6N2E2_2366 at 2800.878 kb on - strand at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2801.664 kb on - strand, within Pf6N2E2_2367 at 2802.616 kb on + strand, within Pf6N2E2_2369 at 2802.617 kb on - strand, within Pf6N2E2_2369 at 2802.694 kb on + strand, within Pf6N2E2_2369 at 2802.695 kb on - strand, within Pf6N2E2_2369
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,800,004 + Pf6N2E2_2365 0.88 +0.4 2,800,005 - Pf6N2E2_2365 0.88 -0.8 2,800,370 + Pf6N2E2_2366 0.78 -1.6 2,800,371 - Pf6N2E2_2366 0.78 -0.4 2,800,371 - Pf6N2E2_2366 0.78 -0.6 2,800,371 - Pf6N2E2_2366 0.78 +0.4 2,800,371 - Pf6N2E2_2366 0.78 -0.8 2,800,384 + Pf6N2E2_2366 0.82 +0.4 2,800,385 - Pf6N2E2_2366 0.82 -0.6 2,800,878 - -0.6 2,801,664 - Pf6N2E2_2367 0.76 -2.6 2,801,664 - Pf6N2E2_2367 0.76 -0.6 2,802,616 + Pf6N2E2_2369 0.25 -0.6 2,802,617 - Pf6N2E2_2369 0.25 -0.6 2,802,694 + Pf6N2E2_2369 0.31 -0.3 2,802,695 - Pf6N2E2_2369 0.31 -0.4
Or see this region's nucleotide sequence