Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2320

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2318 and Pf6N2E2_2319 are separated by 24 nucleotidesPf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotidesPf6N2E2_2320 and Pf6N2E2_2321 are separated by 120 nucleotides Pf6N2E2_2318 - Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,740,743 to 2,743,193 _2318 Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436 _2319 Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112 _2320 Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615 _2321 Position (kb) 2743 2744 2745Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2743.020 kb on + strandat 2743.020 kb on + strandat 2743.020 kb on + strandat 2743.021 kb on - strandat 2743.021 kb on - strandat 2743.021 kb on - strandat 2743.021 kb on - strandat 2743.062 kb on - strandat 2743.334 kb on + strand, within Pf6N2E2_2319at 2743.582 kb on + strand, within Pf6N2E2_2320at 2743.582 kb on + strand, within Pf6N2E2_2320at 2743.606 kb on + strand, within Pf6N2E2_2320at 2743.607 kb on - strand, within Pf6N2E2_2320at 2743.607 kb on - strand, within Pf6N2E2_2320at 2743.717 kb on + strand, within Pf6N2E2_2320at 2743.718 kb on - strand, within Pf6N2E2_2320at 2744.194 kb on + strandat 2744.195 kb on - strandat 2744.296 kb on + strandat 2744.402 kb on - strand, within Pf6N2E2_2321at 2744.412 kb on - strand, within Pf6N2E2_2321at 2744.412 kb on - strand, within Pf6N2E2_2321at 2744.434 kb on + strand, within Pf6N2E2_2321at 2744.435 kb on - strand, within Pf6N2E2_2321at 2744.438 kb on - strand, within Pf6N2E2_2321at 2744.438 kb on - strand, within Pf6N2E2_2321at 2744.438 kb on - strand, within Pf6N2E2_2321at 2744.488 kb on + strand, within Pf6N2E2_2321at 2744.669 kb on - strand, within Pf6N2E2_2321at 2744.866 kb on - strand, within Pf6N2E2_2321at 2744.968 kb on + strand, within Pf6N2E2_2321at 2744.969 kb on - strand, within Pf6N2E2_2321at 2744.969 kb on - strand, within Pf6N2E2_2321at 2745.094 kb on + strand, within Pf6N2E2_2321at 2745.095 kb on - strand, within Pf6N2E2_2321at 2745.095 kb on - strand, within Pf6N2E2_2321

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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2,743,020 + -3.5
2,743,020 + -3.5
2,743,020 + -3.3
2,743,021 - -3.9
2,743,021 - +0.4
2,743,021 - -2.8
2,743,021 - -3.1
2,743,062 - -3.3
2,743,334 + Pf6N2E2_2319 0.53 -2.6
2,743,582 + Pf6N2E2_2320 0.22 -1.9
2,743,582 + Pf6N2E2_2320 0.22 -1.6
2,743,606 + Pf6N2E2_2320 0.26 -1.6
2,743,607 - Pf6N2E2_2320 0.26 -2.8
2,743,607 - Pf6N2E2_2320 0.26 -3.1
2,743,717 + Pf6N2E2_2320 0.42 -3.2
2,743,718 - Pf6N2E2_2320 0.42 -2.9
2,744,194 + -0.6
2,744,195 - +0.4
2,744,296 + -0.6
2,744,402 - Pf6N2E2_2321 0.12 +1.0
2,744,412 - Pf6N2E2_2321 0.13 +0.4
2,744,412 - Pf6N2E2_2321 0.13 +1.0
2,744,434 + Pf6N2E2_2321 0.15 +0.4
2,744,435 - Pf6N2E2_2321 0.15 +0.4
2,744,438 - Pf6N2E2_2321 0.15 -0.6
2,744,438 - Pf6N2E2_2321 0.15 -0.4
2,744,438 - Pf6N2E2_2321 0.15 +0.4
2,744,488 + Pf6N2E2_2321 0.18 +0.4
2,744,669 - Pf6N2E2_2321 0.32 +0.9
2,744,866 - Pf6N2E2_2321 0.46 +0.4
2,744,968 + Pf6N2E2_2321 0.53 +0.4
2,744,969 - Pf6N2E2_2321 0.53 -0.6
2,744,969 - Pf6N2E2_2321 0.53 +0.6
2,745,094 + Pf6N2E2_2321 0.62 -0.6
2,745,095 - Pf6N2E2_2321 0.62 +0.0
2,745,095 - Pf6N2E2_2321 0.62 +0.6

Or see this region's nucleotide sequence