Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2318 and Pf6N2E2_2319 are separated by 24 nucleotides Pf6N2E2_2319 and Pf6N2E2_2320 overlap by 8 nucleotides Pf6N2E2_2320 and Pf6N2E2_2321 are separated by 120 nucleotides
Pf6N2E2_2318 - Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4), at 2,740,743 to 2,743,193
_2318
Pf6N2E2_2319 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 2,743,218 to 2,743,436
_2319
Pf6N2E2_2320 - Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein, at 2,743,429 to 2,744,112
_2320
Pf6N2E2_2321 - Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22), at 2,744,233 to 2,745,615
_2321
Position (kb)
2743
2744
2745 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.020 kb on + strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.021 kb on - strand at 2743.062 kb on - strand at 2743.334 kb on + strand, within Pf6N2E2_2319 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.582 kb on + strand, within Pf6N2E2_2320 at 2743.606 kb on + strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.607 kb on - strand, within Pf6N2E2_2320 at 2743.717 kb on + strand, within Pf6N2E2_2320 at 2743.718 kb on - strand, within Pf6N2E2_2320 at 2744.194 kb on + strand at 2744.195 kb on - strand at 2744.296 kb on + strand at 2744.402 kb on - strand, within Pf6N2E2_2321 at 2744.412 kb on - strand, within Pf6N2E2_2321 at 2744.412 kb on - strand, within Pf6N2E2_2321 at 2744.434 kb on + strand, within Pf6N2E2_2321 at 2744.435 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.438 kb on - strand, within Pf6N2E2_2321 at 2744.488 kb on + strand, within Pf6N2E2_2321 at 2744.669 kb on - strand, within Pf6N2E2_2321 at 2744.866 kb on - strand, within Pf6N2E2_2321 at 2744.968 kb on + strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2744.969 kb on - strand, within Pf6N2E2_2321 at 2745.094 kb on + strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321 at 2745.095 kb on - strand, within Pf6N2E2_2321
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,743,020 + -3.5 2,743,020 + -3.5 2,743,020 + -3.3 2,743,021 - -3.9 2,743,021 - +0.4 2,743,021 - -2.8 2,743,021 - -3.1 2,743,062 - -3.3 2,743,334 + Pf6N2E2_2319 0.53 -2.6 2,743,582 + Pf6N2E2_2320 0.22 -1.9 2,743,582 + Pf6N2E2_2320 0.22 -1.6 2,743,606 + Pf6N2E2_2320 0.26 -1.6 2,743,607 - Pf6N2E2_2320 0.26 -2.8 2,743,607 - Pf6N2E2_2320 0.26 -3.1 2,743,717 + Pf6N2E2_2320 0.42 -3.2 2,743,718 - Pf6N2E2_2320 0.42 -2.9 2,744,194 + -0.6 2,744,195 - +0.4 2,744,296 + -0.6 2,744,402 - Pf6N2E2_2321 0.12 +1.0 2,744,412 - Pf6N2E2_2321 0.13 +0.4 2,744,412 - Pf6N2E2_2321 0.13 +1.0 2,744,434 + Pf6N2E2_2321 0.15 +0.4 2,744,435 - Pf6N2E2_2321 0.15 +0.4 2,744,438 - Pf6N2E2_2321 0.15 -0.6 2,744,438 - Pf6N2E2_2321 0.15 -0.4 2,744,438 - Pf6N2E2_2321 0.15 +0.4 2,744,488 + Pf6N2E2_2321 0.18 +0.4 2,744,669 - Pf6N2E2_2321 0.32 +0.9 2,744,866 - Pf6N2E2_2321 0.46 +0.4 2,744,968 + Pf6N2E2_2321 0.53 +0.4 2,744,969 - Pf6N2E2_2321 0.53 -0.6 2,744,969 - Pf6N2E2_2321 0.53 +0.6 2,745,094 + Pf6N2E2_2321 0.62 -0.6 2,745,095 - Pf6N2E2_2321 0.62 +0.0 2,745,095 - Pf6N2E2_2321 0.62 +0.6
Or see this region's nucleotide sequence