Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2300

Experiment: Ying_AminoAcid20 rep B; time point 3

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Ying_AminoAcid20 rep B; time point 3
2,721,920	+		Pf6N2E2_2298	0.62	-0.7
2,722,029	-		Pf6N2E2_2298	0.70	-0.9
2,722,085	+		Pf6N2E2_2298	0.75	-1.2
2,722,085	+		Pf6N2E2_2298	0.75	-0.2
2,722,085	+		Pf6N2E2_2298	0.75	+0.0
2,722,086	-		Pf6N2E2_2298	0.75	+0.3
2,722,086	-		Pf6N2E2_2298	0.75	+0.3
2,722,086	-		Pf6N2E2_2298	0.75	+0.3
2,722,086	-		Pf6N2E2_2298	0.75	-0.1
2,722,086	-		Pf6N2E2_2298	0.75	-0.7
2,722,134	-		Pf6N2E2_2298	0.78	+0.3
2,722,677	-				+0.3
2,722,677	-				+0.3
2,722,926	+				+0.3
2,722,927	-				-0.7
2,722,927	-				+0.3
2,723,794	+		Pf6N2E2_2300	0.87	-1.9
2,723,794	+		Pf6N2E2_2300	0.87	-0.7
2,723,795	-		Pf6N2E2_2300	0.87	-1.4
2,724,005	+				+0.3
2,724,025	+				+0.3
2,724,025	+				+0.3
2,724,025	+				-2.2
2,724,025	+				+0.3
2,724,026	-				+0.3
2,724,026	-				-0.1
2,724,042	+				-0.7
2,724,042	+				+0.3
2,724,043	-				-0.7
2,724,089	+				+0.3
2,724,089	+				+0.3
2,724,089	+				+0.3
2,724,089	+				-0.6
2,724,090	-				-0.7
2,724,090	-				-0.7
2,724,162	+				+0.3
2,724,162	+				-0.7
2,724,162	+				-1.2
2,724,163	-				+0.3
2,724,163	-				+0.3
2,724,163	-				-0.2
2,724,168	+				-0.7
2,724,168	+				+0.3
2,724,168	+				+0.3
2,724,168	+				+0.3
2,724,168	+				+0.3
2,724,169	-				-0.7
2,724,169	-				-0.7
2,724,345	+				-0.7
2,724,574	-		Pf6N2E2_2301	0.17	-1.7
2,724,663	+		Pf6N2E2_2301	0.20	-0.8
2,724,877	+		Pf6N2E2_2301	0.28	+0.3