Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_229

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_227 and Pf6N2E2_228 are separated by 21 nucleotidesPf6N2E2_228 and Pf6N2E2_229 are separated by 2 nucleotidesPf6N2E2_229 and Pf6N2E2_230 are separated by 345 nucleotides Pf6N2E2_227 - 3-(3-hydroxy-phenyl)propionate hydroxylase (EC 1.14.13.-), at 288,729 to 290,375 _227 Pf6N2E2_228 - Transcriptional regulator, IclR family, at 290,397 to 291,218 _228 Pf6N2E2_229 - Putative fumarylacetoacetate (FAA) hydrolase, at 291,221 to 292,072 _229 Pf6N2E2_230 - lignostilbene-alpha,beta-dioxygenase, at 292,418 to 293,866 _230 Position (kb) 291 292 293Strain fitness (log2 ratio) -2 -1 0 1at 290.379 kb on + strandat 290.379 kb on + strandat 290.582 kb on - strand, within Pf6N2E2_228at 290.618 kb on - strand, within Pf6N2E2_228at 290.618 kb on - strand, within Pf6N2E2_228at 290.618 kb on - strand, within Pf6N2E2_228at 290.618 kb on - strand, within Pf6N2E2_228at 290.618 kb on - strand, within Pf6N2E2_228at 290.618 kb on - strand, within Pf6N2E2_228at 290.801 kb on - strand, within Pf6N2E2_228at 290.970 kb on - strand, within Pf6N2E2_228at 290.993 kb on - strand, within Pf6N2E2_228at 290.993 kb on - strand, within Pf6N2E2_228at 291.208 kb on - strandat 291.208 kb on - strandat 291.208 kb on - strandat 291.208 kb on - strandat 291.208 kb on - strandat 291.208 kb on - strandat 291.208 kb on - strandat 291.223 kb on - strandat 291.607 kb on - strand, within Pf6N2E2_229at 291.607 kb on - strand, within Pf6N2E2_229at 291.607 kb on - strand, within Pf6N2E2_229at 291.784 kb on - strand, within Pf6N2E2_229at 291.826 kb on - strand, within Pf6N2E2_229at 292.032 kb on + strandat 292.032 kb on + strandat 292.032 kb on + strandat 292.032 kb on + strandat 292.033 kb on - strandat 292.036 kb on + strandat 292.037 kb on - strandat 292.037 kb on - strandat 292.039 kb on + strandat 292.055 kb on - strandat 292.055 kb on - strandat 292.110 kb on - strandat 292.196 kb on - strandat 292.196 kb on - strandat 292.337 kb on + strandat 292.338 kb on - strandat 292.388 kb on + strandat 292.388 kb on + strandat 292.388 kb on + strandat 292.389 kb on - strandat 292.389 kb on - strandat 292.570 kb on - strand, within Pf6N2E2_230at 292.570 kb on - strand, within Pf6N2E2_230at 292.570 kb on - strand, within Pf6N2E2_230at 292.709 kb on + strand, within Pf6N2E2_230at 292.710 kb on - strand, within Pf6N2E2_230at 292.731 kb on + strand, within Pf6N2E2_230at 292.732 kb on - strand, within Pf6N2E2_230at 292.732 kb on - strand, within Pf6N2E2_230at 292.789 kb on - strand, within Pf6N2E2_230at 292.789 kb on - strand, within Pf6N2E2_230at 292.821 kb on + strand, within Pf6N2E2_230at 292.822 kb on - strand, within Pf6N2E2_230at 292.822 kb on - strand, within Pf6N2E2_230at 292.916 kb on + strand, within Pf6N2E2_230at 292.916 kb on + strand, within Pf6N2E2_230at 292.917 kb on - strand, within Pf6N2E2_230at 292.920 kb on + strand, within Pf6N2E2_230at 292.921 kb on - strand, within Pf6N2E2_230at 292.944 kb on + strand, within Pf6N2E2_230at 292.944 kb on + strand, within Pf6N2E2_230at 292.945 kb on - strand, within Pf6N2E2_230at 292.945 kb on - strand, within Pf6N2E2_230at 292.945 kb on - strand, within Pf6N2E2_230at 292.945 kb on - strand, within Pf6N2E2_230at 292.980 kb on + strand, within Pf6N2E2_230at 292.980 kb on + strand, within Pf6N2E2_230at 292.980 kb on + strand, within Pf6N2E2_230at 292.980 kb on + strand, within Pf6N2E2_230at 292.980 kb on + strand, within Pf6N2E2_230at 292.981 kb on - strand, within Pf6N2E2_230at 292.981 kb on - strand, within Pf6N2E2_230at 293.014 kb on + strand, within Pf6N2E2_230at 293.015 kb on - strand, within Pf6N2E2_230

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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290,379 + +0.3
290,379 + -0.7
290,582 - Pf6N2E2_228 0.23 -0.5
290,618 - Pf6N2E2_228 0.27 +0.3
290,618 - Pf6N2E2_228 0.27 -1.3
290,618 - Pf6N2E2_228 0.27 -1.0
290,618 - Pf6N2E2_228 0.27 +0.3
290,618 - Pf6N2E2_228 0.27 +0.3
290,618 - Pf6N2E2_228 0.27 +0.3
290,801 - Pf6N2E2_228 0.49 +0.3
290,970 - Pf6N2E2_228 0.70 +0.3
290,993 - Pf6N2E2_228 0.73 -0.7
290,993 - Pf6N2E2_228 0.73 +0.3
291,208 - -1.3
291,208 - -0.7
291,208 - +0.3
291,208 - -0.7
291,208 - +0.3
291,208 - -0.7
291,208 - -0.3
291,223 - +0.3
291,607 - Pf6N2E2_229 0.45 -0.7
291,607 - Pf6N2E2_229 0.45 +0.3
291,607 - Pf6N2E2_229 0.45 +0.3
291,784 - Pf6N2E2_229 0.66 -2.0
291,826 - Pf6N2E2_229 0.71 -1.6
292,032 + +0.3
292,032 + -0.7
292,032 + -0.7
292,032 + +0.3
292,033 - -0.9
292,036 + -0.7
292,037 - -0.7
292,037 - +0.3
292,039 + +0.3
292,055 - -1.3
292,055 - -0.7
292,110 - +1.3
292,196 - +0.3
292,196 - -0.8
292,337 + +0.3
292,338 - +0.3
292,388 + -1.3
292,388 + -2.0
292,388 + -0.7
292,389 - +0.3
292,389 - -1.7
292,570 - Pf6N2E2_230 0.10 -0.8
292,570 - Pf6N2E2_230 0.10 -1.3
292,570 - Pf6N2E2_230 0.10 -0.5
292,709 + Pf6N2E2_230 0.20 +0.3
292,710 - Pf6N2E2_230 0.20 +0.3
292,731 + Pf6N2E2_230 0.22 +0.3
292,732 - Pf6N2E2_230 0.22 -0.0
292,732 - Pf6N2E2_230 0.22 -0.7
292,789 - Pf6N2E2_230 0.26 +0.3
292,789 - Pf6N2E2_230 0.26 -0.7
292,821 + Pf6N2E2_230 0.28 +0.3
292,822 - Pf6N2E2_230 0.28 +0.3
292,822 - Pf6N2E2_230 0.28 +0.3
292,916 + Pf6N2E2_230 0.34 +0.3
292,916 + Pf6N2E2_230 0.34 +0.3
292,917 - Pf6N2E2_230 0.34 -0.6
292,920 + Pf6N2E2_230 0.35 -0.8
292,921 - Pf6N2E2_230 0.35 +0.3
292,944 + Pf6N2E2_230 0.36 +0.3
292,944 + Pf6N2E2_230 0.36 -0.7
292,945 - Pf6N2E2_230 0.36 +0.3
292,945 - Pf6N2E2_230 0.36 +0.3
292,945 - Pf6N2E2_230 0.36 +0.3
292,945 - Pf6N2E2_230 0.36 -0.7
292,980 + Pf6N2E2_230 0.39 +0.3
292,980 + Pf6N2E2_230 0.39 +0.3
292,980 + Pf6N2E2_230 0.39 -0.7
292,980 + Pf6N2E2_230 0.39 +0.3
292,980 + Pf6N2E2_230 0.39 -0.5
292,981 - Pf6N2E2_230 0.39 +0.3
292,981 - Pf6N2E2_230 0.39 +0.3
293,014 + Pf6N2E2_230 0.41 -0.7
293,015 - Pf6N2E2_230 0.41 +0.2

Or see this region's nucleotide sequence