Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2251 and Pf6N2E2_2252 are separated by 7 nucleotides Pf6N2E2_2252 and Pf6N2E2_2253 are separated by 262 nucleotides Pf6N2E2_2253 and Pf6N2E2_2254 overlap by 1 nucleotides
Pf6N2E2_2251 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 2,665,694 to 2,666,578
_2251
Pf6N2E2_2252 - NreA-like protein, at 2,666,586 to 2,666,873
_2252
Pf6N2E2_2253 - hypothetical protein, at 2,667,136 to 2,667,306
_2253
Pf6N2E2_2254 - Cell filamentation protein fic, at 2,667,306 to 2,667,905
_2254
Position (kb)
2667
2668 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2666.249 kb on - strand, within Pf6N2E2_2251 at 2666.307 kb on + strand, within Pf6N2E2_2251 at 2666.308 kb on - strand, within Pf6N2E2_2251 at 2666.346 kb on + strand, within Pf6N2E2_2251 at 2666.347 kb on - strand, within Pf6N2E2_2251 at 2666.347 kb on - strand, within Pf6N2E2_2251 at 2666.347 kb on - strand, within Pf6N2E2_2251 at 2666.409 kb on - strand, within Pf6N2E2_2251 at 2666.409 kb on - strand, within Pf6N2E2_2251 at 2666.526 kb on + strand at 2666.527 kb on - strand at 2666.527 kb on - strand at 2666.527 kb on - strand at 2666.588 kb on - strand at 2666.588 kb on - strand at 2666.593 kb on + strand at 2666.593 kb on + strand at 2666.593 kb on + strand at 2666.593 kb on + strand at 2666.594 kb on - strand at 2666.594 kb on - strand at 2666.594 kb on - strand at 2666.594 kb on - strand at 2666.676 kb on + strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.677 kb on - strand, within Pf6N2E2_2252 at 2666.783 kb on + strand, within Pf6N2E2_2252 at 2666.784 kb on - strand, within Pf6N2E2_2252 at 2666.901 kb on + strand at 2666.902 kb on - strand at 2666.902 kb on - strand at 2666.978 kb on + strand at 2666.978 kb on + strand at 2666.978 kb on + strand at 2666.979 kb on - strand at 2666.979 kb on - strand at 2667.021 kb on + strand at 2667.076 kb on + strand at 2667.076 kb on + strand at 2667.076 kb on + strand at 2667.077 kb on - strand at 2667.099 kb on + strand at 2667.099 kb on + strand at 2667.099 kb on + strand at 2667.101 kb on + strand at 2667.102 kb on - strand at 2667.144 kb on + strand at 2667.169 kb on + strand, within Pf6N2E2_2253 at 2667.169 kb on + strand, within Pf6N2E2_2253 at 2667.186 kb on + strand, within Pf6N2E2_2253 at 2667.196 kb on + strand, within Pf6N2E2_2253 at 2667.197 kb on - strand, within Pf6N2E2_2253 at 2667.197 kb on - strand, within Pf6N2E2_2253 at 2667.318 kb on + strand at 2667.318 kb on + strand at 2667.318 kb on + strand at 2667.318 kb on + strand at 2667.319 kb on - strand at 2667.319 kb on - strand at 2667.319 kb on - strand at 2667.340 kb on - strand at 2667.340 kb on - strand at 2667.401 kb on - strand, within Pf6N2E2_2254 at 2667.466 kb on - strand, within Pf6N2E2_2254 at 2667.466 kb on - strand, within Pf6N2E2_2254 at 2667.478 kb on - strand, within Pf6N2E2_2254 at 2667.585 kb on + strand, within Pf6N2E2_2254 at 2667.585 kb on + strand, within Pf6N2E2_2254 at 2667.585 kb on + strand, within Pf6N2E2_2254 at 2667.585 kb on + strand, within Pf6N2E2_2254 at 2667.586 kb on - strand at 2667.706 kb on + strand, within Pf6N2E2_2254 at 2667.707 kb on - strand, within Pf6N2E2_2254 at 2667.707 kb on - strand, within Pf6N2E2_2254 at 2667.837 kb on + strand, within Pf6N2E2_2254 at 2667.837 kb on + strand, within Pf6N2E2_2254 at 2667.937 kb on + strand at 2668.101 kb on + strand at 2668.176 kb on + strand at 2668.276 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,666,249 - Pf6N2E2_2251 0.63 +0.3 2,666,307 + Pf6N2E2_2251 0.69 +0.3 2,666,308 - Pf6N2E2_2251 0.69 -0.7 2,666,346 + Pf6N2E2_2251 0.74 -0.7 2,666,347 - Pf6N2E2_2251 0.74 +0.3 2,666,347 - Pf6N2E2_2251 0.74 -0.7 2,666,347 - Pf6N2E2_2251 0.74 +0.3 2,666,409 - Pf6N2E2_2251 0.81 -0.7 2,666,409 - Pf6N2E2_2251 0.81 +0.3 2,666,526 + -0.7 2,666,527 - +0.3 2,666,527 - +0.3 2,666,527 - -0.7 2,666,588 - +0.3 2,666,588 - +0.3 2,666,593 + -0.6 2,666,593 + -1.7 2,666,593 + +0.3 2,666,593 + -0.4 2,666,594 - +0.3 2,666,594 - -0.3 2,666,594 - +0.3 2,666,594 - -0.5 2,666,676 + Pf6N2E2_2252 0.31 -0.9 2,666,677 - Pf6N2E2_2252 0.32 +0.3 2,666,677 - Pf6N2E2_2252 0.32 +0.3 2,666,677 - Pf6N2E2_2252 0.32 -0.7 2,666,677 - Pf6N2E2_2252 0.32 +0.3 2,666,677 - Pf6N2E2_2252 0.32 +0.3 2,666,677 - Pf6N2E2_2252 0.32 -0.5 2,666,677 - Pf6N2E2_2252 0.32 +0.3 2,666,783 + Pf6N2E2_2252 0.68 -0.9 2,666,784 - Pf6N2E2_2252 0.69 +0.3 2,666,901 + +0.3 2,666,902 - +0.3 2,666,902 - -0.7 2,666,978 + +0.3 2,666,978 + -0.5 2,666,978 + +0.3 2,666,979 - -0.7 2,666,979 - +0.3 2,667,021 + -0.8 2,667,076 + -0.7 2,667,076 + -0.7 2,667,076 + +0.3 2,667,077 - +0.3 2,667,099 + -1.3 2,667,099 + +0.3 2,667,099 + +0.3 2,667,101 + -0.7 2,667,102 - +0.3 2,667,144 + +0.3 2,667,169 + Pf6N2E2_2253 0.19 +0.3 2,667,169 + Pf6N2E2_2253 0.19 -0.7 2,667,186 + Pf6N2E2_2253 0.29 -2.0 2,667,196 + Pf6N2E2_2253 0.35 +0.3 2,667,197 - Pf6N2E2_2253 0.36 -0.7 2,667,197 - Pf6N2E2_2253 0.36 -0.7 2,667,318 + +0.3 2,667,318 + -2.8 2,667,318 + +0.3 2,667,318 + -0.3 2,667,319 - +0.3 2,667,319 - +0.3 2,667,319 - +0.3 2,667,340 - -1.7 2,667,340 - +0.3 2,667,401 - Pf6N2E2_2254 0.16 +0.3 2,667,466 - Pf6N2E2_2254 0.27 +0.3 2,667,466 - Pf6N2E2_2254 0.27 +0.3 2,667,478 - Pf6N2E2_2254 0.29 +0.3 2,667,585 + Pf6N2E2_2254 0.47 +0.3 2,667,585 + Pf6N2E2_2254 0.47 +0.3 2,667,585 + Pf6N2E2_2254 0.47 -0.5 2,667,585 + Pf6N2E2_2254 0.47 -0.7 2,667,586 - +0.3 2,667,706 + Pf6N2E2_2254 0.67 +0.3 2,667,707 - Pf6N2E2_2254 0.67 -0.7 2,667,707 - Pf6N2E2_2254 0.67 +0.3 2,667,837 + Pf6N2E2_2254 0.89 +0.3 2,667,837 + Pf6N2E2_2254 0.89 +0.3 2,667,937 + +0.3 2,668,101 + -0.7 2,668,176 + -0.7 2,668,276 + +0.3
Or see this region's nucleotide sequence