Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2146 and Pf6N2E2_2147 are separated by 115 nucleotides Pf6N2E2_2147 and Pf6N2E2_2148 overlap by 4 nucleotides Pf6N2E2_2148 and Pf6N2E2_2149 are separated by 297 nucleotides
Pf6N2E2_2146 - Porphobilinogen synthase (EC 4.2.1.24), at 2,551,988 to 2,552,962
_2146
Pf6N2E2_2147 - Phenazine biosynthesis protein PhzF like, at 2,553,078 to 2,553,935
_2147
Pf6N2E2_2148 - Glutathione S-transferase (EC 2.5.1.18), at 2,553,932 to 2,554,630
_2148
Pf6N2E2_2149 - contains type I hydrophobic transmembrane region and ATP/GTP binding motif, at 2,554,928 to 2,555,623
_2149
Position (kb)
2553
2554
2555 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.980 kb on + strand at 2552.981 kb on - strand at 2553.008 kb on + strand at 2553.008 kb on + strand at 2553.009 kb on - strand at 2553.034 kb on + strand at 2553.035 kb on - strand at 2553.057 kb on - strand at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.199 kb on + strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.200 kb on - strand, within Pf6N2E2_2147 at 2553.728 kb on + strand, within Pf6N2E2_2147 at 2553.729 kb on - strand, within Pf6N2E2_2147 at 2553.904 kb on + strand at 2553.904 kb on + strand at 2553.904 kb on + strand at 2553.905 kb on - strand at 2553.905 kb on - strand at 2553.905 kb on - strand at 2554.042 kb on - strand, within Pf6N2E2_2148 at 2554.096 kb on - strand, within Pf6N2E2_2148 at 2554.173 kb on + strand, within Pf6N2E2_2148 at 2554.173 kb on + strand, within Pf6N2E2_2148 at 2554.173 kb on + strand, within Pf6N2E2_2148 at 2554.174 kb on - strand, within Pf6N2E2_2148 at 2554.174 kb on - strand, within Pf6N2E2_2148 at 2554.200 kb on + strand, within Pf6N2E2_2148 at 2554.344 kb on + strand, within Pf6N2E2_2148 at 2554.560 kb on + strand, within Pf6N2E2_2148 at 2554.560 kb on + strand, within Pf6N2E2_2148 at 2554.561 kb on - strand at 2554.678 kb on + strand at 2554.678 kb on + strand at 2554.678 kb on + strand at 2554.679 kb on - strand at 2554.707 kb on - strand at 2554.759 kb on + strand at 2554.759 kb on + strand at 2554.760 kb on - strand at 2554.779 kb on + strand at 2554.779 kb on + strand at 2554.780 kb on - strand at 2554.780 kb on - strand at 2554.939 kb on + strand at 2554.939 kb on + strand at 2554.939 kb on + strand at 2554.940 kb on - strand at 2554.940 kb on - strand at 2554.940 kb on - strand at 2554.943 kb on + strand at 2554.944 kb on - strand at 2554.944 kb on - strand at 2554.944 kb on - strand at 2555.210 kb on + strand, within Pf6N2E2_2149 at 2555.211 kb on - strand, within Pf6N2E2_2149 at 2555.211 kb on - strand, within Pf6N2E2_2149 at 2555.211 kb on - strand, within Pf6N2E2_2149 at 2555.267 kb on + strand, within Pf6N2E2_2149 at 2555.267 kb on + strand, within Pf6N2E2_2149 at 2555.268 kb on - strand, within Pf6N2E2_2149 at 2555.269 kb on + strand, within Pf6N2E2_2149 at 2555.269 kb on + strand, within Pf6N2E2_2149 at 2555.270 kb on - strand, within Pf6N2E2_2149 at 2555.270 kb on - strand, within Pf6N2E2_2149 at 2555.270 kb on - strand, within Pf6N2E2_2149 at 2555.270 kb on - strand, within Pf6N2E2_2149 at 2555.270 kb on - strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.273 kb on + strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.274 kb on - strand, within Pf6N2E2_2149 at 2555.276 kb on - strand, within Pf6N2E2_2149 at 2555.276 kb on - strand, within Pf6N2E2_2149 at 2555.276 kb on - strand, within Pf6N2E2_2149 at 2555.339 kb on + strand, within Pf6N2E2_2149 at 2555.340 kb on - strand, within Pf6N2E2_2149 at 2555.340 kb on - strand, within Pf6N2E2_2149 at 2555.390 kb on + strand, within Pf6N2E2_2149 at 2555.391 kb on - strand, within Pf6N2E2_2149 at 2555.391 kb on - strand, within Pf6N2E2_2149 at 2555.408 kb on + strand, within Pf6N2E2_2149 at 2555.408 kb on + strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.409 kb on - strand, within Pf6N2E2_2149 at 2555.430 kb on - strand, within Pf6N2E2_2149 at 2555.525 kb on + strand, within Pf6N2E2_2149 at 2555.525 kb on + strand, within Pf6N2E2_2149 at 2555.526 kb on - strand, within Pf6N2E2_2149 at 2555.526 kb on - strand, within Pf6N2E2_2149 at 2555.526 kb on - strand, within Pf6N2E2_2149 at 2555.579 kb on + strand at 2555.579 kb on + strand at 2555.580 kb on - strand at 2555.580 kb on - strand at 2555.580 kb on - strand at 2555.580 kb on - strand at 2555.580 kb on - strand at 2555.580 kb on - strand at 2555.580 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,552,980 + +0.3 2,552,980 + +0.3 2,552,980 + -1.1 2,552,980 + +0.3 2,552,980 + +0.3 2,552,981 - -1.3 2,553,008 + +0.3 2,553,008 + +0.3 2,553,009 - -0.7 2,553,034 + -0.3 2,553,035 - -0.6 2,553,057 - -0.5 2,553,199 + Pf6N2E2_2147 0.14 -0.7 2,553,199 + Pf6N2E2_2147 0.14 -0.7 2,553,199 + Pf6N2E2_2147 0.14 -0.3 2,553,199 + Pf6N2E2_2147 0.14 -0.7 2,553,199 + Pf6N2E2_2147 0.14 -2.5 2,553,199 + Pf6N2E2_2147 0.14 +0.3 2,553,199 + Pf6N2E2_2147 0.14 -0.7 2,553,199 + Pf6N2E2_2147 0.14 -0.7 2,553,199 + Pf6N2E2_2147 0.14 +0.3 2,553,199 + Pf6N2E2_2147 0.14 +0.3 2,553,199 + Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -0.5 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -0.7 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -0.7 2,553,200 - Pf6N2E2_2147 0.14 -2.0 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -2.9 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -0.7 2,553,200 - Pf6N2E2_2147 0.14 -1.7 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 -0.7 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 +0.3 2,553,200 - Pf6N2E2_2147 0.14 +0.9 2,553,728 + Pf6N2E2_2147 0.76 +0.3 2,553,729 - Pf6N2E2_2147 0.76 -1.5 2,553,904 + -0.7 2,553,904 + +0.3 2,553,904 + -0.7 2,553,905 - -0.4 2,553,905 - +0.3 2,553,905 - +0.3 2,554,042 - Pf6N2E2_2148 0.16 -0.3 2,554,096 - Pf6N2E2_2148 0.23 -0.7 2,554,173 + Pf6N2E2_2148 0.34 +0.3 2,554,173 + Pf6N2E2_2148 0.34 +0.3 2,554,173 + Pf6N2E2_2148 0.34 -0.7 2,554,174 - Pf6N2E2_2148 0.35 +0.3 2,554,174 - Pf6N2E2_2148 0.35 -0.7 2,554,200 + Pf6N2E2_2148 0.38 -1.4 2,554,344 + Pf6N2E2_2148 0.59 +0.3 2,554,560 + Pf6N2E2_2148 0.90 -0.7 2,554,560 + Pf6N2E2_2148 0.90 +0.3 2,554,561 - +0.3 2,554,678 + -0.6 2,554,678 + -3.0 2,554,678 + +0.3 2,554,679 - +0.3 2,554,707 - +0.3 2,554,759 + +0.3 2,554,759 + +0.3 2,554,760 - -0.7 2,554,779 + +0.3 2,554,779 + +0.3 2,554,780 - +0.3 2,554,780 - +0.3 2,554,939 + -1.7 2,554,939 + +0.3 2,554,939 + +0.3 2,554,940 - -0.7 2,554,940 - -0.4 2,554,940 - -0.7 2,554,943 + -0.7 2,554,944 - +0.3 2,554,944 - -0.7 2,554,944 - -0.0 2,555,210 + Pf6N2E2_2149 0.41 -0.7 2,555,211 - Pf6N2E2_2149 0.41 -2.0 2,555,211 - Pf6N2E2_2149 0.41 -0.7 2,555,211 - Pf6N2E2_2149 0.41 -0.7 2,555,267 + Pf6N2E2_2149 0.49 -1.3 2,555,267 + Pf6N2E2_2149 0.49 -0.7 2,555,268 - Pf6N2E2_2149 0.49 +0.3 2,555,269 + Pf6N2E2_2149 0.49 -0.6 2,555,269 + Pf6N2E2_2149 0.49 +0.3 2,555,270 - Pf6N2E2_2149 0.49 +0.3 2,555,270 - Pf6N2E2_2149 0.49 -1.3 2,555,270 - Pf6N2E2_2149 0.49 -0.5 2,555,270 - Pf6N2E2_2149 0.49 +0.3 2,555,270 - Pf6N2E2_2149 0.49 -0.7 2,555,273 + Pf6N2E2_2149 0.50 +0.3 2,555,273 + Pf6N2E2_2149 0.50 -0.7 2,555,273 + Pf6N2E2_2149 0.50 -1.6 2,555,273 + Pf6N2E2_2149 0.50 +0.3 2,555,273 + Pf6N2E2_2149 0.50 +0.3 2,555,273 + Pf6N2E2_2149 0.50 -0.7 2,555,273 + Pf6N2E2_2149 0.50 +0.3 2,555,273 + Pf6N2E2_2149 0.50 +0.3 2,555,273 + Pf6N2E2_2149 0.50 -1.3 2,555,274 - Pf6N2E2_2149 0.50 -0.7 2,555,274 - Pf6N2E2_2149 0.50 -0.9 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 -0.8 2,555,274 - Pf6N2E2_2149 0.50 -0.7 2,555,274 - Pf6N2E2_2149 0.50 -1.3 2,555,274 - Pf6N2E2_2149 0.50 -1.3 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 -3.0 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 -2.9 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 +0.3 2,555,274 - Pf6N2E2_2149 0.50 -0.4 2,555,274 - Pf6N2E2_2149 0.50 -1.7 2,555,274 - Pf6N2E2_2149 0.50 -0.5 2,555,274 - Pf6N2E2_2149 0.50 -0.4 2,555,276 - Pf6N2E2_2149 0.50 +0.3 2,555,276 - Pf6N2E2_2149 0.50 +0.3 2,555,276 - Pf6N2E2_2149 0.50 -1.3 2,555,339 + Pf6N2E2_2149 0.59 -0.7 2,555,340 - Pf6N2E2_2149 0.59 -1.0 2,555,340 - Pf6N2E2_2149 0.59 -0.7 2,555,390 + Pf6N2E2_2149 0.66 +0.0 2,555,391 - Pf6N2E2_2149 0.67 -0.4 2,555,391 - Pf6N2E2_2149 0.67 +0.3 2,555,408 + Pf6N2E2_2149 0.69 +0.3 2,555,408 + Pf6N2E2_2149 0.69 +0.3 2,555,409 - Pf6N2E2_2149 0.69 -1.3 2,555,409 - Pf6N2E2_2149 0.69 +0.3 2,555,409 - Pf6N2E2_2149 0.69 +0.3 2,555,409 - Pf6N2E2_2149 0.69 +0.3 2,555,409 - Pf6N2E2_2149 0.69 -0.4 2,555,409 - Pf6N2E2_2149 0.69 -2.0 2,555,409 - Pf6N2E2_2149 0.69 +0.3 2,555,409 - Pf6N2E2_2149 0.69 -0.7 2,555,409 - Pf6N2E2_2149 0.69 +0.3 2,555,430 - Pf6N2E2_2149 0.72 +0.3 2,555,525 + Pf6N2E2_2149 0.86 +0.3 2,555,525 + Pf6N2E2_2149 0.86 +0.3 2,555,526 - Pf6N2E2_2149 0.86 +0.3 2,555,526 - Pf6N2E2_2149 0.86 +0.3 2,555,526 - Pf6N2E2_2149 0.86 +0.3 2,555,579 + -0.7 2,555,579 + +0.3 2,555,580 - -0.7 2,555,580 - -1.7 2,555,580 - -0.7 2,555,580 - -1.3 2,555,580 - +1.3 2,555,580 - -0.7 2,555,580 - -1.3
Or see this region's nucleotide sequence