Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2033 and Pf6N2E2_2034 are separated by 5 nucleotides Pf6N2E2_2034 and Pf6N2E2_2035 are separated by 67 nucleotides
Pf6N2E2_2033 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 2,421,833 to 2,423,425
_2033
Pf6N2E2_2034 - Methyltransferase, at 2,423,431 to 2,424,294
_2034
Pf6N2E2_2035 - epoxide hydrolase, at 2,424,362 to 2,425,366
_2035
Position (kb)
2423
2424
2425 Strain fitness (log2 ratio)
-2
-1
0
1 at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.589 kb on + strand, within Pf6N2E2_2033 at 2422.590 kb on - strand, within Pf6N2E2_2033 at 2422.721 kb on + strand, within Pf6N2E2_2033 at 2422.722 kb on - strand, within Pf6N2E2_2033 at 2422.741 kb on + strand, within Pf6N2E2_2033 at 2422.742 kb on - strand, within Pf6N2E2_2033 at 2423.249 kb on + strand, within Pf6N2E2_2033 at 2423.261 kb on + strand, within Pf6N2E2_2033 at 2423.261 kb on + strand, within Pf6N2E2_2033 at 2423.262 kb on - strand, within Pf6N2E2_2033 at 2423.262 kb on - strand, within Pf6N2E2_2033 at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.387 kb on + strand at 2423.388 kb on - strand at 2423.388 kb on - strand at 2423.389 kb on + strand at 2423.390 kb on - strand at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.567 kb on - strand, within Pf6N2E2_2034 at 2423.889 kb on + strand, within Pf6N2E2_2034 at 2424.013 kb on - strand, within Pf6N2E2_2034 at 2424.275 kb on + strand at 2424.364 kb on - strand at 2424.364 kb on - strand at 2424.600 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.603 kb on + strand, within Pf6N2E2_2035 at 2424.604 kb on - strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.644 kb on + strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.645 kb on - strand, within Pf6N2E2_2035 at 2424.771 kb on + strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.772 kb on - strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.888 kb on + strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035 at 2424.889 kb on - strand, within Pf6N2E2_2035
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,422,589 + Pf6N2E2_2033 0.47 -0.7 2,422,589 + Pf6N2E2_2033 0.47 +0.3 2,422,590 - Pf6N2E2_2033 0.48 -0.7 2,422,721 + Pf6N2E2_2033 0.56 -0.7 2,422,722 - Pf6N2E2_2033 0.56 -1.0 2,422,741 + Pf6N2E2_2033 0.57 -0.3 2,422,742 - Pf6N2E2_2033 0.57 -0.5 2,423,249 + Pf6N2E2_2033 0.89 +0.3 2,423,261 + Pf6N2E2_2033 0.90 -0.7 2,423,261 + Pf6N2E2_2033 0.90 +0.3 2,423,262 - Pf6N2E2_2033 0.90 +0.3 2,423,262 - Pf6N2E2_2033 0.90 -0.7 2,423,387 + -0.1 2,423,387 + -0.7 2,423,387 + -0.7 2,423,388 - -0.7 2,423,388 - -0.5 2,423,389 + -1.3 2,423,390 - -0.7 2,423,567 - Pf6N2E2_2034 0.16 +0.3 2,423,567 - Pf6N2E2_2034 0.16 +0.3 2,423,889 + Pf6N2E2_2034 0.53 -2.7 2,424,013 - Pf6N2E2_2034 0.67 -0.7 2,424,275 + -0.4 2,424,364 - -1.3 2,424,364 - -0.7 2,424,600 + Pf6N2E2_2035 0.24 -0.7 2,424,603 + Pf6N2E2_2035 0.24 -0.5 2,424,603 + Pf6N2E2_2035 0.24 -0.7 2,424,603 + Pf6N2E2_2035 0.24 -0.1 2,424,604 - Pf6N2E2_2035 0.24 -0.7 2,424,644 + Pf6N2E2_2035 0.28 -0.3 2,424,644 + Pf6N2E2_2035 0.28 -0.7 2,424,644 + Pf6N2E2_2035 0.28 +0.3 2,424,645 - Pf6N2E2_2035 0.28 +0.3 2,424,645 - Pf6N2E2_2035 0.28 -0.6 2,424,645 - Pf6N2E2_2035 0.28 -1.2 2,424,645 - Pf6N2E2_2035 0.28 -0.5 2,424,771 + Pf6N2E2_2035 0.41 -0.5 2,424,772 - Pf6N2E2_2035 0.41 -1.3 2,424,772 - Pf6N2E2_2035 0.41 +0.3 2,424,772 - Pf6N2E2_2035 0.41 +0.3 2,424,772 - Pf6N2E2_2035 0.41 +0.3 2,424,772 - Pf6N2E2_2035 0.41 +0.3 2,424,772 - Pf6N2E2_2035 0.41 -0.7 2,424,888 + Pf6N2E2_2035 0.52 -0.7 2,424,888 + Pf6N2E2_2035 0.52 -0.7 2,424,888 + Pf6N2E2_2035 0.52 -1.0 2,424,888 + Pf6N2E2_2035 0.52 -0.7 2,424,889 - Pf6N2E2_2035 0.52 +0.3 2,424,889 - Pf6N2E2_2035 0.52 +0.3
Or see this region's nucleotide sequence