Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1965 and Pf6N2E2_1966 are separated by 841 nucleotides Pf6N2E2_1966 and Pf6N2E2_1967 are separated by 452 nucleotides
Pf6N2E2_1965 - Fructokinase (EC 2.7.1.4) (from data) , at 2,330,391 to 2,331,311
_1965
Pf6N2E2_1966 - SAM-dependent methyltransferases, at 2,332,153 to 2,332,833
_1966
Pf6N2E2_1967 - Cobalt-zinc-cadmium resistance protein, at 2,333,286 to 2,333,915
_1967
Position (kb)
2332
2333 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2331.173 kb on - strand, within Pf6N2E2_1965 at 2331.564 kb on + strand at 2331.570 kb on + strand at 2331.572 kb on + strand at 2331.642 kb on + strand at 2331.643 kb on - strand at 2331.643 kb on - strand at 2331.707 kb on + strand at 2331.726 kb on - strand at 2331.726 kb on - strand at 2331.726 kb on - strand at 2331.786 kb on + strand at 2331.787 kb on - strand at 2331.835 kb on + strand at 2331.836 kb on - strand at 2331.899 kb on - strand at 2331.911 kb on + strand at 2331.911 kb on + strand at 2331.914 kb on - strand at 2331.929 kb on + strand at 2331.929 kb on + strand at 2331.930 kb on - strand at 2332.102 kb on + strand at 2332.103 kb on - strand at 2332.103 kb on - strand at 2332.213 kb on + strand at 2332.217 kb on + strand at 2332.217 kb on + strand at 2332.218 kb on - strand at 2332.593 kb on + strand, within Pf6N2E2_1966 at 2332.611 kb on + strand, within Pf6N2E2_1966 at 2332.611 kb on + strand, within Pf6N2E2_1966 at 2332.612 kb on - strand, within Pf6N2E2_1966 at 2332.716 kb on - strand, within Pf6N2E2_1966 at 2332.785 kb on + strand at 2332.795 kb on + strand at 2332.817 kb on + strand at 2332.854 kb on + strand at 2332.854 kb on + strand at 2332.927 kb on + strand at 2332.928 kb on - strand at 2332.951 kb on + strand at 2332.951 kb on + strand at 2333.124 kb on - strand at 2333.124 kb on - strand at 2333.159 kb on - strand at 2333.193 kb on - strand at 2333.213 kb on - strand at 2333.213 kb on - strand at 2333.244 kb on + strand at 2333.245 kb on - strand at 2333.254 kb on + strand at 2333.254 kb on + strand at 2333.254 kb on + strand at 2333.254 kb on + strand at 2333.255 kb on - strand at 2333.255 kb on - strand at 2333.255 kb on - strand at 2333.255 kb on - strand at 2333.470 kb on + strand, within Pf6N2E2_1967 at 2333.470 kb on + strand, within Pf6N2E2_1967 at 2333.470 kb on + strand, within Pf6N2E2_1967 at 2333.471 kb on - strand, within Pf6N2E2_1967 at 2333.471 kb on - strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.479 kb on + strand, within Pf6N2E2_1967 at 2333.480 kb on - strand, within Pf6N2E2_1967 at 2333.499 kb on + strand, within Pf6N2E2_1967 at 2333.500 kb on - strand, within Pf6N2E2_1967 at 2333.562 kb on + strand, within Pf6N2E2_1967 at 2333.563 kb on - strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.647 kb on + strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.648 kb on - strand, within Pf6N2E2_1967 at 2333.801 kb on - strand, within Pf6N2E2_1967 at 2333.809 kb on + strand, within Pf6N2E2_1967
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,331,173 - Pf6N2E2_1965 0.85 -0.7 2,331,564 + +0.3 2,331,570 + -0.7 2,331,572 + +0.3 2,331,642 + +0.3 2,331,643 - +0.3 2,331,643 - +0.5 2,331,707 + -0.7 2,331,726 - +0.3 2,331,726 - -2.5 2,331,726 - -0.7 2,331,786 + -0.7 2,331,787 - +0.3 2,331,835 + -0.7 2,331,836 - +0.3 2,331,899 - +0.3 2,331,911 + -0.7 2,331,911 + +0.3 2,331,914 - +0.3 2,331,929 + -0.9 2,331,929 + +0.3 2,331,930 - -0.7 2,332,102 + -0.7 2,332,103 - +0.3 2,332,103 - +0.3 2,332,213 + -0.7 2,332,217 + +0.3 2,332,217 + +1.9 2,332,218 - -0.4 2,332,593 + Pf6N2E2_1966 0.65 -0.7 2,332,611 + Pf6N2E2_1966 0.67 -0.7 2,332,611 + Pf6N2E2_1966 0.67 +0.3 2,332,612 - Pf6N2E2_1966 0.67 +0.3 2,332,716 - Pf6N2E2_1966 0.83 -0.7 2,332,785 + -0.7 2,332,795 + +0.3 2,332,817 + +0.3 2,332,854 + -1.3 2,332,854 + -0.7 2,332,927 + -0.4 2,332,928 - -0.7 2,332,951 + -0.7 2,332,951 + -0.7 2,333,124 - -1.2 2,333,124 - +0.3 2,333,159 - +0.3 2,333,193 - +0.3 2,333,213 - +0.3 2,333,213 - +0.3 2,333,244 + +0.3 2,333,245 - +0.3 2,333,254 + +0.3 2,333,254 + -1.5 2,333,254 + +0.3 2,333,254 + +0.3 2,333,255 - -1.1 2,333,255 - +0.3 2,333,255 - +0.3 2,333,255 - +0.3 2,333,470 + Pf6N2E2_1967 0.29 +0.3 2,333,470 + Pf6N2E2_1967 0.29 +0.3 2,333,470 + Pf6N2E2_1967 0.29 -0.7 2,333,471 - Pf6N2E2_1967 0.29 -0.7 2,333,471 - Pf6N2E2_1967 0.29 -0.7 2,333,479 + Pf6N2E2_1967 0.31 +0.3 2,333,479 + Pf6N2E2_1967 0.31 +0.3 2,333,479 + Pf6N2E2_1967 0.31 -1.3 2,333,479 + Pf6N2E2_1967 0.31 +0.3 2,333,479 + Pf6N2E2_1967 0.31 +0.3 2,333,480 - Pf6N2E2_1967 0.31 +0.3 2,333,499 + Pf6N2E2_1967 0.34 -0.7 2,333,500 - Pf6N2E2_1967 0.34 +0.3 2,333,562 + Pf6N2E2_1967 0.44 +0.3 2,333,563 - Pf6N2E2_1967 0.44 +0.3 2,333,647 + Pf6N2E2_1967 0.57 -1.0 2,333,647 + Pf6N2E2_1967 0.57 +0.3 2,333,647 + Pf6N2E2_1967 0.57 +0.3 2,333,647 + Pf6N2E2_1967 0.57 +0.3 2,333,647 + Pf6N2E2_1967 0.57 +0.3 2,333,647 + Pf6N2E2_1967 0.57 +0.3 2,333,648 - Pf6N2E2_1967 0.57 -0.7 2,333,648 - Pf6N2E2_1967 0.57 +0.3 2,333,648 - Pf6N2E2_1967 0.57 +0.9 2,333,648 - Pf6N2E2_1967 0.57 +0.3 2,333,648 - Pf6N2E2_1967 0.57 -0.7 2,333,648 - Pf6N2E2_1967 0.57 +0.3 2,333,801 - Pf6N2E2_1967 0.82 +0.3 2,333,809 + Pf6N2E2_1967 0.83 -0.7
Or see this region's nucleotide sequence