Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1875

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1874 and Pf6N2E2_1875 are separated by 50 nucleotidesPf6N2E2_1875 and Pf6N2E2_1876 are separated by 44 nucleotides Pf6N2E2_1874 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,221,551 to 2,222,990 _1874 Pf6N2E2_1875 - Protoporphyrinogen oxidase (EC 1.3.3.4), at 2,223,041 to 2,224,369 _1875 Pf6N2E2_1876 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49), at 2,224,414 to 2,225,805 _1876 Position (kb) 2223 2224 2225Strain fitness (log2 ratio) -2 -1 0 1at 2222.711 kb on - strand, within Pf6N2E2_1874at 2222.892 kb on + strandat 2222.929 kb on - strandat 2223.001 kb on - strandat 2223.137 kb on + strandat 2223.138 kb on - strandat 2223.160 kb on - strandat 2223.304 kb on + strand, within Pf6N2E2_1875at 2223.573 kb on - strand, within Pf6N2E2_1875at 2223.759 kb on + strand, within Pf6N2E2_1875at 2224.249 kb on + strandat 2224.315 kb on - strandat 2224.396 kb on - strandat 2224.454 kb on - strandat 2224.542 kb on + strandat 2224.543 kb on - strandat 2224.543 kb on - strandat 2224.672 kb on + strand, within Pf6N2E2_1876at 2224.673 kb on - strand, within Pf6N2E2_1876at 2225.113 kb on - strand, within Pf6N2E2_1876at 2225.230 kb on - strand, within Pf6N2E2_1876

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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2,222,711 - Pf6N2E2_1874 0.81 -0.7
2,222,892 + +0.3
2,222,929 - -0.7
2,223,001 - +0.3
2,223,137 + -1.3
2,223,138 - +0.3
2,223,160 - -0.7
2,223,304 + Pf6N2E2_1875 0.20 -0.7
2,223,573 - Pf6N2E2_1875 0.40 +0.3
2,223,759 + Pf6N2E2_1875 0.54 -0.7
2,224,249 + -2.1
2,224,315 - +0.3
2,224,396 - -2.1
2,224,454 - -1.0
2,224,542 + -0.4
2,224,543 - +0.3
2,224,543 - -0.9
2,224,672 + Pf6N2E2_1876 0.19 +0.3
2,224,673 - Pf6N2E2_1876 0.19 +0.3
2,225,113 - Pf6N2E2_1876 0.50 +0.3
2,225,230 - Pf6N2E2_1876 0.59 -0.7

Or see this region's nucleotide sequence