Experiment: Ying_AminoAcid20 rep B; time point 3
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1801 and Pf6N2E2_1802 are separated by 150 nucleotides Pf6N2E2_1802 and Pf6N2E2_1803 overlap by 8 nucleotides Pf6N2E2_1803 and Pf6N2E2_1804 overlap by 4 nucleotides
Pf6N2E2_1801 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 2,130,740 to 2,131,564
_1801
Pf6N2E2_1802 - polysaccharide deacetylase, at 2,131,715 to 2,132,563
_1802
Pf6N2E2_1803 - Polysaccharide deacetylase, at 2,132,556 to 2,133,443
_1803
Pf6N2E2_1804 - Immune-responsive protein 1, at 2,133,440 to 2,134,834
_1804
Position (kb)
2132
2133
2134 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2131.678 kb on + strand at 2131.678 kb on + strand at 2131.679 kb on - strand at 2131.687 kb on + strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.751 kb on + strand at 2131.751 kb on + strand at 2131.752 kb on - strand at 2131.838 kb on + strand, within Pf6N2E2_1802 at 2131.945 kb on - strand, within Pf6N2E2_1802 at 2132.176 kb on + strand, within Pf6N2E2_1802 at 2132.177 kb on - strand, within Pf6N2E2_1802 at 2132.181 kb on - strand, within Pf6N2E2_1802 at 2132.214 kb on - strand, within Pf6N2E2_1802 at 2132.282 kb on + strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.403 kb on + strand, within Pf6N2E2_1802 at 2132.502 kb on + strand at 2133.114 kb on + strand, within Pf6N2E2_1803 at 2133.115 kb on - strand, within Pf6N2E2_1803 at 2133.172 kb on - strand, within Pf6N2E2_1803 at 2133.234 kb on + strand, within Pf6N2E2_1803 at 2133.235 kb on - strand, within Pf6N2E2_1803 at 2133.440 kb on - strand at 2133.541 kb on + strand at 2134.345 kb on - strand, within Pf6N2E2_1804 at 2134.424 kb on + strand, within Pf6N2E2_1804 at 2134.425 kb on - strand, within Pf6N2E2_1804
Per-strain Table
Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3 remove 2,131,678 + +0.2 2,131,678 + -0.8 2,131,679 - -2.8 2,131,687 + -2.8 2,131,688 - -0.8 2,131,688 - +0.2 2,131,688 - +0.2 2,131,751 + -0.7 2,131,751 + -0.8 2,131,752 - +0.2 2,131,838 + Pf6N2E2_1802 0.14 -1.1 2,131,945 - Pf6N2E2_1802 0.27 +0.4 2,132,176 + Pf6N2E2_1802 0.54 +1.8 2,132,177 - Pf6N2E2_1802 0.54 +0.2 2,132,181 - Pf6N2E2_1802 0.55 -0.3 2,132,214 - Pf6N2E2_1802 0.59 +0.2 2,132,282 + Pf6N2E2_1802 0.67 -0.8 2,132,298 - Pf6N2E2_1802 0.69 +0.2 2,132,298 - Pf6N2E2_1802 0.69 +0.2 2,132,403 + Pf6N2E2_1802 0.81 +0.2 2,132,502 + -0.8 2,133,114 + Pf6N2E2_1803 0.63 -1.3 2,133,115 - Pf6N2E2_1803 0.63 +0.2 2,133,172 - Pf6N2E2_1803 0.69 +0.2 2,133,234 + Pf6N2E2_1803 0.76 +0.2 2,133,235 - Pf6N2E2_1803 0.76 -1.8 2,133,440 - +0.2 2,133,541 + +0.2 2,134,345 - Pf6N2E2_1804 0.65 +0.2 2,134,424 + Pf6N2E2_1804 0.71 +0.2 2,134,425 - Pf6N2E2_1804 0.71 +0.2
Or see this region's nucleotide sequence