Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_178

Experiment: Ying_AminoAcid20 rep B; time point 3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_177 and Pf6N2E2_178 are separated by 35 nucleotidesPf6N2E2_178 and Pf6N2E2_179 are separated by 3 nucleotidesPf6N2E2_179 and Pf6N2E2_180 overlap by 20 nucleotidesPf6N2E2_180 and Pf6N2E2_6104 are separated by 90 nucleotides Pf6N2E2_177 - Phenylalanyl-tRNA synthetase alpha chain (EC 6.1.1.20), at 226,871 to 227,887 _177 Pf6N2E2_178 - Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20), at 227,923 to 230,301 _178 Pf6N2E2_179 - Integration host factor alpha subunit, at 230,305 to 230,607 _179 Pf6N2E2_180 - Transcriptional regulator, MerR family, at 230,588 to 230,944 _180 Pf6N2E2_6104 - tRNA-Pro-GGG, at 231,035 to 231,108 _6104 Position (kb) 227 228 229 230 231Strain fitness (log2 ratio) -1 0 1at 229.328 kb on - strand, within Pf6N2E2_178at 230.304 kb on + strandat 230.304 kb on + strandat 230.304 kb on + strandat 230.305 kb on - strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.587 kb on + strandat 230.588 kb on - strandat 230.588 kb on - strandat 230.605 kb on + strandat 230.756 kb on + strand, within Pf6N2E2_180at 230.756 kb on + strand, within Pf6N2E2_180at 230.756 kb on + strand, within Pf6N2E2_180at 230.804 kb on + strand, within Pf6N2E2_180at 230.869 kb on + strand, within Pf6N2E2_180at 230.870 kb on - strand, within Pf6N2E2_180at 231.128 kb on - strandat 231.218 kb on - strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.283 kb on + strandat 231.284 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Ying_AminoAcid20 rep B; time point 3
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229,328 - Pf6N2E2_178 0.59 -1.3
230,304 + -0.7
230,304 + -0.7
230,304 + +0.3
230,305 - +0.3
230,587 + -0.7
230,587 + -1.3
230,587 + +0.3
230,587 + -0.7
230,587 + +0.3
230,588 - +0.3
230,588 - +0.3
230,605 + -1.3
230,756 + Pf6N2E2_180 0.47 +0.3
230,756 + Pf6N2E2_180 0.47 -1.5
230,756 + Pf6N2E2_180 0.47 +0.3
230,804 + Pf6N2E2_180 0.61 -0.7
230,869 + Pf6N2E2_180 0.79 +0.3
230,870 - Pf6N2E2_180 0.79 -0.7
231,128 - -0.7
231,218 - -0.4
231,283 + -1.1
231,283 + -0.7
231,283 + -0.7
231,283 + +0.3
231,283 + -0.7
231,283 + -0.7
231,284 - -0.8

Or see this region's nucleotide sequence